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in subjects: "Cell Flow Systems"

open access

Characterization of normal and neoplastic mammalian cells by multiparameter flow-system analysis methods

A flow-systems technique for rapid measurement of nuclear and cytoplasmic size relationships in normal and neoplastic mammalian cell populations, including human exfoliated gynecological specimens, has been developed. Based on fluorescence staining of either the nucleus alone or in combination with the cytoplasm using two-color fluorescence methods, this technique permits the simultaneous determination of nuclear and cytoplasmic diameters from fluorescence and light-scatter measurements. Cells … more

Date: January 1, 1976

Creator: Steinkamp, J. A.; Hansen, K. M. & Crissman, H. A.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Review of rapid cell analysis and automation of disease diagnosis using flow microfluorometry

Rapid single-cell flow-analysis techniques such as flow microfluorometry (FMF) have been shown to have clinical applications in microbiology, virology, immunology, and cell biology. The potential capabilities of such a handling system have always been numerous and are now rapidly becoming proven capabilities as the number of institutions involved in rapid cell analysis and sorting increases. The purpose of this review will be to discuss briefly the operational features of the instrumentation an… more

Date: January 1, 1976

Creator: Cram, L. S.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Laser backscattering measurements on biological cells: preliminary results

A cell flow-system instrument is described in which the light scattered by a cell as it passes through a focused helium-neon laser beam is detected simultaneously at 26 angles between 0/sup 0/ and 4.4/sup 0/ and at 176/sup 0/ with respect to the laser beam axis. The usefulness of backscatter in distinguishing among variously sized plastic microspheres is demonstrated. Preliminary results with mouse bone marrow cells are also discussed.

Date: January 1, 1976

Creator: Salzman, G. C.; Price, B. J.; Hiebert, R. D.; Mullaney, P. F. & Visser, J. W. M.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Flow-system analysis of exfoliated pulmonary cells: results of initial characterization studies in hamsters

This paper summarizes results of preliminary experiments to develop cytological and biochemical indicators for estimating damage to respiratory cells in test animals exposed by inhalation to toxic agents associated with nonnuclear energy production, the specific goal being the application of advanced multiparameter flow-systems technologies to the detection of early atypical cellular changes in lung epithelium. Normal Syrian hamster lung cell samples composed of histiocytes, leukocytes, macroph… more

Date: January 1, 1976

Creator: Steinkamp, J. A.; Hansen, K. M.; Wilson, J. S. & Salzman, G. C.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Discrimination of proliferating from nonproliferating cells and single-cell enzyme measurements with flow systems

With the progression of flow systems into clinical medicine, the number of applications directed toward characterization of cancer at the cellular level is growing rapidly. This paper presents the highlights of two such applications. The assessment of cell proliferation is crucial and can now be approached using the advantages of flow systems to measure indirectly the incorporation of a base analog into those cells that are synthesizing DNA. A new technique for evaluating the immune mechanism a… more

Date: January 1, 1977

Creator: Cram, L. S.; Swartzendruber, D. E. & Wilder, M. E.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

(Controls of the plant endomembrane-secretory pathway)

These studies are focused on elucidating the molecular structure of plant cell membranes with special reference to cell surface glycoproteins. The studies reported herein include use of monoclonal antibodies to characterize cell surface epitopes, construction of cDNA libraries of cell surface proteins, isolation of plant cell mutants by flow cytometry, detection of beta-glucouronidase marker enzyme systems in plants, expression go VSVG (the major envelope glycoprotein of Vesicular Stomatis Viru… more

Date: January 1, 1991

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

[Vertically intergrated analysis of human DNA]. Progress report, [April 1, 1993--February 28, 1994]

Progress is reported on several fronts aimed at DNA sequencing using cosmids-contigs. Specifically results gained in exploiting RARE (recA-assisted-restriction enzyme) cleavage, in clone isolation by flow cytometry, in cosmid-DNA preparations, in automated interpretation of digitized gel images and in contig building via multiple complete digest (MCD) mapping are briefly described.

Date: March 1, 1994

Creator: Olson, M. V.

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

Laser-based blood cell measurements for the biological reconstruction of ionizing radiation doses using a novel technique for cytometry/biochemical reaction analysis

A novel biodosimetry method based on the red blood cells analysis by means of laser flow cytometry and a specially designed monoclonal antibodies is used for reconstructing ionizing radiation doses for Chernobyl Accident victims. In addition to analysis of the patient`s blood samples, we are evaluating both spectroscopy procedures and laser-based flow techniques. It was shown that resonance energy transfer processes can take place among the two different dyes links to the spherical cell surface… more

Date: January 1, 1994

Creator: Pleshanov, P. G.; Langlois, R. G.; Azarov, L. A.; Budayev, D. A.; Ossipenkov, A. L. & Shamayev, D. M.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

DM-CSF stimulated proliferation of rat alveolar macrophages and the effects of differing particulate burden levels in alveolar macrophages on the proliferative response

Particles are gradually redistributed among the lung`s population of alveolar macrophages (AM) at times well after they were originally deposited in the lung. One mechanism that may underlie this ``particle redistribution phenomenon`` is the in situ replication of particle-containing AM and the allocation of particles from dividing AM to daughter cells. Little is known about how the abilities of AM to proliferate may be affected by the containment of particles; conceivably, AM proliferation may… more

Date: May 1, 1993

Creator: Pendergrass, D.; Valdez, Y. E. & Lehnert, B. E.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Flow cytogenetics: Fundamentals and new developments

The purpose of this report is to provide (1) an introduction to flow cytogenetics and (2) resource information for additional and more detailed information for those interested in either using or further developing the technology. Several recent reviews are available and provide in-depth information on all aspects of flow cytogenetics, this information will by and large, not be repeated here. Examples of common types of flow cytogenetic data are included along with an explanation of their inter… more

Date: June 1, 1992

Creator: Cram, L. S.; Fawcett, J. & Deaven, L. L.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

[Controls of the plant endomembrane-secretory pathway]. Final report

Date: December 31, 1991

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

Flow cytometric fluorescence lifetime analysis of DNA binding fluorochromes

Most flow cytometry (FCM) applications monitor fluorescence intensity to quantitate the various cellular parameters; however, the fluorescence emission also contains information relative to the fluorescence lifetime. Recent developments in FCM (Pinsky et al., 1993; Steinkamp & Crissman, 1993; Steinkamp et al., 1993), provide for the measurement of fluorescence lifetime which is also commonly referred to as fluorescence decay, or the time interval in which a fluorochrome remains in the excited s… more

Date: January 1, 2002

Creator: Crissman, Harry A.; Cui, H. H. (H. Helen) & Steinkamp, J. A.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Magnetic Tools for Lab-on-a-chip Technologies

This study establishes a set of magnetics-based tools that have been integrated with microfluidic systems. The overall impact of the work begins to enable the rapid and efficient manipulation and detection of magnetic entities such as particles, picoliter-sized droplets, or bacterial cells. Details of design, fabrication, and theoretical and experimental assessments are presented. The manipulation strategy has been demonstrated in the format of a particle diverter, whereby micron-sized particle… more

Date: August 5, 2006

Creator: Pekas, Nikola Slobodan

Item Type: Thesis or Dissertation

Partner: UNT Libraries Government Documents Department

open access

The Autonomous Pathogen Detection System

We developed, tested, and now operate a civilian biological defense capability that continuously monitors the air for biological threat agents. The Autonomous Pathogen Detection System (APDS) collects, prepares, reads, analyzes, and reports results of multiplexed immunoassays and multiplexed PCR assays using Luminex{copyright} xMAP technology and flow cytometer. The mission we conduct is particularly demanding: continuous monitoring, multiple threat agents, high sensitivity, challenging environ… more

Date: January 13, 2009

Creator: Dzenitis, J M & Makarewicz, A J

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Instrumentation for biomedical and environmental applications based on microtechnology lessons learned

Over the last ten years, LLNL has been developing Microtechnology for instrumentation with applications in the biosciences and environment. In order to build and field high-performance instruments, they have often had to alter their original premises and assumptions, significantly. This meant that they were forced to abandon materials and dimensions that were appealing to them when they began the R and D. Examples include work on silicon-based electrophoresis systems, etched-fluidics for sample… more

Date: January 1, 1999

Creator: Mariella, Jr., R P

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

DNA fragment sizing and sorting by laser-induced fluorescence

A method is provided for obtaining DNA fingerprints using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a selected sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to … more

Date: December 31, 1992

Creator: Jett, J. H.; Hammond, M. L.; Keller, R. A.; Marrone, B. L. & Martin, J. C.

Item Type: Patent

Partner: UNT Libraries Government Documents Department

open access

Biopsy examination during the course of radiotherapy. Progress report. [DNA content of tumor cells in biopsy samples determined by microfluorometric or multiangle light-scatter spectrometry]

Studies begun during the first year of this project on biopsies from spontaneous tumors in dogs and from human patients undergoing radiotherapy treatment were continued. The results indicate that significant fractions of the spontaneous tumors in dogs and cervical carcinomas in patients undergoing radiotherapy have a DNA content higher than normal diploid cells. The higher DNA content in tumor cells permits distinguishing them from normal cells in the biopsy material with the aid of flow microf… more

Date: December 1, 1977

Creator: Raju, M. R.

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

Flow cytogenetics: Fundamentals and new developments

Date: January 1, 1992

Creator: Cram, L.S.; Fawcett, J. & Deaven, L.L.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Critical tonicity determination of sperm using fluorescent staining and flow cytometry

The use of cryopreserved, rather than fresh, mammalian semen for artificial insemination confers several important medical and/or economic advantages. However, current methods for cryopreservation of both human and bovine spermatozoa result in approximately only a 50% survival rate with thawing, obviously reducing the fertilizing capacity of the semen. A primary consideration during the cooling process is to avoid intracellular ice crystal formation with its lethal consequences to the cell. Cur… more

Date: January 1, 1990

Creator: Noiles, E.E.; Ruffing, N.A.; Kleinhans, F.W.; Mark, L.A.; Watson, P.F.; Critser, J.K. (Methodist Hospital, Indianapolis, IN (USA)) et al.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Flow cytometry: an overview

Flow cytometry has progressed considerably since its inception a decade ago, and major advances not only in measurement techniques, data acquisition, and data analysis but also in areas of biomedical applications appear promising. Flow systems are essentially a marriage between physical techniques and appropriate biological or cytochemical techniques. The dual nature of the method must not be overlooked, particularly by the new user. With the appropriate mating of interests between instrumentat… more

Date: January 1, 1978

Creator: Mullaney, P.F. & Jett, J.H.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Human semen assays for workplace monitoring. [Monitoring of hazardous materials by determining effects on semen of personnel]

Decades of human semen studies have yielded compelling evidence that sperm can be used to access reproductive potential and diagnose pathology. With these studies as background, the small number of detailed semen studies of men exposed to physical and chemical agents point with optimism to the application of human semen assays as efficient, effective means to monitor for reproductive hazards in the workplace. Sperm are the most accessible of human gonadal tissue and provide a means of monitorin… more

Date: November 7, 1978

Creator: Wyrobek, A.J. & Gledhill, B.L.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Ultrasensitive flow cytometric analyses

New techniques and approaches to cellular analysis being developed at the Los Alamos National Flow Cytometry Resource can be divided into those that improve sensitivity and those that move the technology into new areas by refining existing approaches. An example of the first category is a flow cytometric system capable of measuring the phase shift of fluorescence emitted by fluorophors bound to cells is being assembled. This phase sensitive cytometer is be capable of quantifying fluorescence li… more

Date: January 1, 1993

Creator: Jett, J.H.; Cram, L.S.; Keller, R.A.; Martin, J.C.; Saunders, G.C.; Sklar, L.A. et al.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Pulsed laser fluorometry for environmental monitoring

A compact pulsed laser fluorometer has been incorporated into a continuous flow system developed to detect acetylcholinesterase (AChE) inhibitors and/or primary amine compounds in air and water. A pulsed nitrogen laser pumped dye laser excites fluorescent reactants which flow continuously through a quartz flow cell. Data are collected, analyzed, and displayed using a Macintosh II personal computer. For detection of cholinesterase inhibitors the fluorogenic substrate N methylindoxyl acetate is u… more

Date: January 1, 1990

Creator: Saunders, G. C.; Martin, J. C.; Jett, J. H.; Wilder, M. E.; Martinez, A.; Bentley, B. F. et al.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Ultrastructural and flow cytometric analyses of lipid accumulation in microalgae

Lipid accumulation in three species of microalgae was investigated with flow cytometry (FCM) and transmission electron microscopy (TEM). Previous studies using batch cultures of a algae have led to the assumption that lipid accumulation in microalgae is a gradual process requiring at least several days for completion. However, FCM reveals, through changes in the chlorophyll:lipid ratio, that the time span required for individual cells to change metabolic state is short. Simultaneous FCM measure… more

Date: December 1, 1986

Creator: Solomon, J. A.; Hand, R. E., Jr. & Mann, R. C.

Item Type: Report

Partner: UNT Libraries Government Documents Department