DNA fragment sizing and sorting by laser-induced fluorescence

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Description

A method is provided for obtaining DNA fingerprints using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a selected sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the ... continued below

Physical Description

19 p.

Creation Information

Jett, J.H.; Hammond, M.L.; Keller, R.A.; Marrone, B.L. & Martin, J.C. December 31, 1992.

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Description

A method is provided for obtaining DNA fingerprints using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a selected sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is directly proportional to the fragment length. Additional dyes can be bound to the DNA piece and DNA fragments to provide information additional to length information. Oligonucleotide specific dyes and/or hybridization probes can be bound to the DNA fragments to provide information on oligonucleotide distribution or probe hybridization to DNA fragments of different sizes.

Physical Description

19 p.

Notes

OSTI as DE94007342

Source

  • Other Information: PBD: 1992

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  • Other: DE94007342
  • Report No.: PATENTS-US--A7841114
  • Grant Number: W-7405-ENG-36
  • Office of Scientific & Technical Information Report Number: 140616
  • Archival Resource Key: ark:/67531/metadc621441

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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Creation Date

  • December 31, 1992

Added to The UNT Digital Library

  • June 16, 2015, 7:43 a.m.

Description Last Updated

  • Feb. 25, 2016, 4:06 p.m.

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Citations, Rights, Re-Use

Jett, J.H.; Hammond, M.L.; Keller, R.A.; Marrone, B.L. & Martin, J.C. DNA fragment sizing and sorting by laser-induced fluorescence, patent, December 31, 1992; New Mexico. (digital.library.unt.edu/ark:/67531/metadc621441/: accessed December 16, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.