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Stability of Myosin Subfragment-2 Modulates the Force Produced by Acto-Myosin Interaction of Striated Muscle

Description: Myosin subfragment-2 (S2) is a coiled coil linker between myosin subfragment-1 and light meromyosin (LMM). This dissertation examines whether the myosin S2 coiled coil could regulate the amount of myosin S1 heads available to bind actin thin filaments by modulating the stability of its coiled coil. A stable myosin S2 coiled coil would have less active myosin S1 heads compared to a more flexible myosin S2 coiled coil, thus causing increased force production through acto-myosin interaction. The … more
Date: December 2017
Creator: Singh, Rohit Rajendraprasad
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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N-Acylethanolamines and Plant Phospholipase D

Description: Recently, three distinct isoforms of phospholipase D (PLD) were identified in Arabidopsis thaliana. PLD α represents the well-known form found in plants, while PLD β and γ have been only recently discovered (Pappan et al., 1997b; Qin et al., 1997). These isoforms differ in substrate selectivity and cofactors required for activity. Here, I report that PLD β and γ isoforms were active toward N-acylphosphatidylethanolamine (NAPE), but PLD α was not. The ability of PLD β and γ to hydrolyze NAPE mar… more
Date: December 1998
Creator: Brown, Shea Austin
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Subcloning and Nucleotide Sequence of the xylO/PUWCMA Region from the Pseudomonas putida TOL Plasmid pDK1

Description: The TOL plasmids of Pseudomonas putida encode enzymes required for the oxidation of toluene and other related aromatic compounds. These genes are organized into two operons, the xylUWCMABN operon (upper), and the xylXYZLTEGFJQKIH operon (lower). Here we report the nucleotide sequence of a 7107 bp segment of the TOL pDK1 plasmid encoding the region just upstream of the "upper" operon through the genes encoding xylUWCMA. Sequence analysis, comparison of base-usage patterns, codon-usage patterns, … more
Date: December 1997
Creator: Guigneaux, Michelle M. (Michelle Marie)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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DNA Typing of HLA-B by PCR with Primer Mixes Utilizing Sequence-Specific Primers

Description: The aim of this study was to design a resolution typing system for the HLA-B gene. This technique involves a one-step PCR reaction utilizing genomic DNA and sequence-specific primers to determine the specificity of each allele and to produce a larger primer data base ideal for serological analysis. The application of this technique to serological analysis can improve serology detection which is currently hindered by antibody cross-reactivity and the unavailability of useful typing reagents.
Date: August 1997
Creator: Chiu, Angela Chen-Yen
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Exon/Intron Discrimination Using the Finite Induction Pattern Matching Technique

Description: DNA sequence analysis involves precise discrimination of two of the sequence's most important components: exons and introns. Exons encode the proteins that are responsible for almost all the functions in a living organism. Introns interrupt the sequence coding for a protein and must be removed from primary RNA transcripts before translation to protein can occur. A pattern recognition technique called Finite Induction (FI) is utilized to study the language of exons and introns. FI is especially… more
Date: December 1997
Creator: Taylor, Pamela A., 1941-
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Characterization of Aspartate Transcarbamoylase and Dihydroorotase in Moraxella Catarrhalis

Description: Bacterial aspartate transcarbamoylases (ATCase's) are divided into three classes that correspond to taxonomic relationships within the bacteria. The opportunistic pathogen Moraxeila catarrhalis has undergone several reclassifications based on traditional microbiological criteria. The previously uncharacterized ATCase from M. catarrhalis was purified to homogeneity and its chemical properties characterized. The ATCase from M. catarrhalis is a class C ATCase with an apparent molecular mass of 480… more
Date: May 1998
Creator: Fowler, Michael A. (Michael Allen), 1961-
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Regulatory Divergence of Aspartate Transcarbamoylase from the Pseudomonads

Description: Aspartate transcarbamoylase (ATCase) was purified from 16 selected bacterial species including existing Pseudomonas species and former species reassigned to new genera. An enormous diversity was seen among the 16 enzymes with each class of ATCase being represented. The smallest class, class C, with a catalytically active homotrimer, at 100 kDa, was found in Bacillus and other Gram positive bacteria. In this report, the ATCases from the Gram negatives, Shewanella putrefaciens and Stenotrophomona… more
Date: December 1996
Creator: Linscott, Andrea J. (Andrea Jane)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Modulation of the Coelomic Fluid Protein Profile in the Earthworm, Lumbricus Terrestris, After Exposure to Copper as Copper Sulfate

Description: Proteomic techniques were used to analyze the protein profile of earthworm, Lumbricus terrestris, coelomic fluid collected by either whole body dissection method or the coelomic cavity puncture method. Data demonstrated that collection of coelomic fluid using the coelomic cavity puncture method protocol resulted in a 32% reduction, 377 +/- 4.5 vs 253+/- 19.9 (p=0.0007), in the number of individual proteins. It was determined that the coelomic cavity puncture method yielded a "cleaner" preparati… more
Date: May 2010
Creator: Herring, Reese
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Stretching the Flexible Myosin II Subfragment Using the Novel Gravitational Force Spectroscope, and the Uncoiling of S2

Description: Familial Hypertrophic cardiomyopathy (HCM) causes ventricle walls to thicken and often leads to sudden death especially in adults. Mutations in the subfragment 2 (S2) of β-cardiac myosin are implicated in the genetic disorder. This S2 region is a coiled-coil rod region resulting from the dimeric form of myosin II. It has been proposed that an elastic quality allows normal S2 to absorb force during the powerstroke according to the sliding filament model. To test the flexibility of single mo… more
Date: May 2010
Creator: Dunn, James W.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Cloning of Carbonic Anhydrase from Cotton (Gossypium hirsutum L.)

Description: Carbonic anhydrase is a ubiquitous zinc-metalloenzyme that catalyzes the interconversion of carbon dioxide and carbonate and has been found to play a wide range of roles in animals, plants and bacteria. Cotton genomic and cDNA libraries were screened for the plastidial isoform of carbonic anhydrase. The nucleotide sequences of two 1.2 Kb partial cDNA clones were determined. These clones exhibit high homology to carbonic anhydrases from other dicot plants and possess all the expected peptide mot… more
Date: December 1998
Creator: Local, Andrea
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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A Novel Mechanism for Site-Directed Mutagenesis of Large Catabolic Plasmids Using Natural Transformation

Description: Natural transformation is the process by which cells take up DNA from the surrounding medium under physiological conditions, altering the genotype in a heritable fashion. This occurs without chemical or physical treatment of the cells. Certain Acinetobacter strains exhibit a strong tendency to incorporate homologous DNA into their chromosomes by natural transformation. Transformation in Acinetobacter exhibits several unique properties that indicate this system's superiority as a model for tra… more
Date: August 2001
Creator: Williamson, Phillip C.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Isolation and analysis of cotton genomic clones encompassing a fatty acid desaturase (FAD2) gene

Description: Polyunsaturated fatty acids are major structural components of plant chloroplast and endoplasmic reticulum membranes. Two fatty acid desaturases (designated FAD2 and FAD3) desaturate 75% of the fatty acids in the endoplasmic reticulum. The w -6 fatty acid desaturase (FAD2) may be responsible for cold acclimation response, since polyunsaturated phospholipids are important in helping maintain plant viability at lowered temperatures. To study regulation of FAD2 gene expression in cotton, a FAD2 g… more
Date: May 2001
Creator: Kongcharoensuntorn, Wisatre
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Novel Role of Trypsin in Zebrafish

Description: It has been shown previously in our laboratory that zebrafish produce trypsin from their gills when they are under stress, and this trypsin is involved in thrombocyte activation via PAR2 during gill bleeding. In this study, I investigated another role of the trypsin that is secreted from zebrafish. This investigation has demonstrated a novel role of trypsin in zebrafish. Not only did this investigation demonstrate the role of trypsin in zebrafish behavior, but also it showed that PAR2 might be … more
Date: May 2013
Creator: Alsrhani, Abdullah Falleh
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries

Characterization of Moraxella bovis Aspartate Transcarbamoylase

Description: Aspartate transcarbamoylase (ATCase) catalyzes the first committed step in the pyrimidine biosynthetic pathway. Bacterial ATCases have been divided into three classes, class A, B, and C, based on their molecular weight, holoenzyme architecture, and enzyme kinetics. Moraxella bovis is a fastidious organism, the etiologic agent of infectious bovine keratoconjunctivitis (IBK). The M. bovis ATCase was purified and characterized for the first time. It is a class A enzyme with a molecular mass of 4… more
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Date: December 2001
Creator: Hooshdaran, Sahar
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Purification of Aspartate Transcarbamoylase from Moraxella (Branhamella) catarrhalis

Description: The enzyme, aspartate transcarbamoylase (ATCase) from Moraxella (Branhamella) catarrhalis, has been purified. The holoenzyme has a molecular mass of approximately 510kDa, harbors predominantly positive charges and is hydrophobic in nature. The holoenzyme possesses two subunits, a smaller one of 40 kDa and a larger one of 45 kDa. A third polypeptide has been found to contribute to the overall enzymatic activity, having an approximate mass of 55 kDa. The ATCase purification included the generati… more
Date: August 2001
Creator: Stawska, Agnieszka A.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Plastidial carbonic anhydrase in cotton (Gossypium hirsutum L.): characterization, expression, and role in lipid biosynthesis

Description: Recently, plastidial carbonic anhydrase (CA, EC 4.2.1.1) cDNA clones encoding functional CA enzymes were isolated from a nonphotosynthetic cotton tissue. The role of CA in photosynthetic tissues have been well characterized, however there is almost no information for the role of CA in nonphotosynthetic tissues. A survey of relative CA transcript abundance and enzyme activity in different cotton organs revealed that there was substantial CA expression in cotyledons of seedlings and embryos, both… more
Date: August 2001
Creator: Hoang, Chau V.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Palmitoyl-acyl Carrier Protein Thioesterase in Cotton (Gossypium hirsutum L.): Biochemical and Molecular Characterization of a Major Mechanism for the Regulation of Palmitic Acid Content

Description: The relatively high level of palmitic acid (22 mol%) in cottonseeds may be due in part to the activity of a palmitoyl-acyl carrier protein (ACP) thioesterase (PATE). In embryo extracts, PATE activity was highest at the maximum rate of reserve accumulation (oil and protein). The cotton FatB mRNA transcript abundance also peaked during this developmental stage, paralleling the profiles of PATE enzyme activity and seed oil accumulation. A cotton FatB cDNA clone was isolated by screening a cDNA lib… more
Date: August 2001
Creator: Huynh, Tu T
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Molecular cloning and analysis of the genes for cotton palmitoyl-acyl carrier protein thioesterase (PATE) and Δ-12 fatty acid desaturase (FAD2-3) and construction of sense and anti-sense PATE plasmid vectors for altering oilseed composition of transgenic cotton plants.

Description: A cotton PATE cDNA clone has a 1.7-kb insert with an coding region for 410 amino acids, lacking codons for the three N-terminal amino acids. The predicted amino acid sequence of the PATE preprotein has a characteristic stromal-targeting domain and a 63% identity to the Arabidopsis FatB1 thioesterase sequence. A cotton genomic clone containing a 17.4-kb DNA segment was found to encompass a palmitoyl-ACP thioesterase (FatB1) gene. The gene spans 3.6 kb with six exons and five introns. The six… more
Date: May 2002
Creator: Nampaisansuk, Mongkol
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Dna Profiling of Captive Roseate Spoonbill (Ajaia Ajaja) Populations As a Mechanism of Determining Lineage in Colonial Nesting Birds.

Description: Roseate spoonbills are colonial nesting birds with breeding grounds extending from the United States Gulf coast to the pampas of Argentina. The U.S. population suffered a severe bottleneck from 1890 to 1920. The population's recovery was slow and partially credited to migrations from Mexican rookeries, but a gene pool reduction would be expected. Five polymorphic Spoonbill autosomal short tandem repeat (STR) loci [three (GAT)n, one (AAAG)n and one (GT)n] and one Z/W-linked microsatellite exhibi… more
Date: May 2002
Creator: Sawyer, Gregory M.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Functional Neural Toxicity and Endocrine Responses in Mice Following Naphthalene Exposure

Description: Polycyclic aromatic hydrocarbons (PAHs) are a well studied and diverse class of environmental toxicants. PAHs act via the aryl hydrocarbon receptor (AhR), and studies have suggested that PAHs may elicit neurological and estrogenic effects. Doses of PAHs between 50 to 150 ppm may elicit neurotoxicity in rodent models. The present study investigated the effects of naphthalene on in vivo steroidogenesis in Swiss Webster male mice, and in vitro neural function of Balb-C/ICR mice frontal cortex ne… more
Date: August 2010
Creator: Colbert, Crystal
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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DNA Degradation as an Indicator of Post-Mortem Interval

Description: The question of post-mortem interval (PMI) or time since death is often the most sought after piece of information associated with a medical death investigation. Based on the observation that DNA degradation disproportionately affects the analysis of larger genetic loci, it was proposed that DNA degradation, as a result of autolysis or putrefaction, could prove suitable as a potential rate-of-change indicator of PMI. Nine randomly amplified polymorphic DNA (RAPD) analysis primers and three se… more
Date: August 2010
Creator: Watson, William H.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Characterization of the Aspartate Transcarbamoylase that is Found in the pyrBC’ Complex of Bordetella Pertussis

Description: An aspartate transcarbamoylase (ATCase) gene from Bordetella pertussis was amplified by PCR and ligated into pT-ADV for expression in Escherichia coli. This particular ATCase (pyrB) was an inactive gene found adjacent to an inactive dihydroorotase (DHOase) gene (pyrC'). This experiment was undertaken to determine whether this pyrB gene was capable of expression alone or if it was capable of expression only when cotransformed with a functional pyrC'. When transformed into E. coli TB2 pyrB-… more
Date: December 2001
Creator: Dill, Michael T
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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A Study of the Pyrimidine Biosynthesis Pathway and its Regulation in Two Distinct Organisms: Methanococcus jannaschii and Pseudomonas aeruginosa

Description: Methanococcus jannaschii is a thermophilic methane producing archaebacterium. In this organism genes encoding the aspartate transcarbamoylase (ATCase) catalytic (PyrB) and regulatory (PyrI) polypeptides were found. Unlike Escherichia coli where the above genes are expressed from a biscistronic operon the two genes in M. jannaschii are separated by 200-kb stretch of genome. Previous researchers have not been able to show regulation of the M. jannaschii enzyme by the nucleotide effectors ATP,… more
Date: December 2001
Creator: Patel, Seema R.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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