DNA Typing of HLA-B by PCR with Primer Mixes Utilizing Sequence-Specific Primers

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Description

The aim of this study was to design a resolution typing system for the HLA-B gene. This technique involves a one-step PCR reaction utilizing genomic DNA and sequence-specific primers to determine the specificity of each allele and to produce a larger primer data base ideal for serological analysis. The application of this technique to serological analysis can improve serology detection which is currently hindered by antibody cross-reactivity and the unavailability of useful typing reagents.

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viii, 74 leaves: ill.

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Chiu, Angela Chen-Yen August 1997.

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This thesis is part of the collection entitled: UNT Theses and Dissertations and was provided by UNT Libraries to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 846 times , with 15 in the last month . More information about this thesis can be viewed below.

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  • Chiu, Angela Chen-Yen

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Description

The aim of this study was to design a resolution typing system for the HLA-B gene. This technique involves a one-step PCR reaction utilizing genomic DNA and sequence-specific primers to determine the specificity of each allele and to produce a larger primer data base ideal for serological analysis. The application of this technique to serological analysis can improve serology detection which is currently hindered by antibody cross-reactivity and the unavailability of useful typing reagents.

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viii, 74 leaves: ill.

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UNT Theses and Dissertations

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  • August 1997

Added to The UNT Digital Library

  • March 26, 2014, 9:30 a.m.

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  • July 22, 2014, 1:47 p.m.

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Chiu, Angela Chen-Yen. DNA Typing of HLA-B by PCR with Primer Mixes Utilizing Sequence-Specific Primers, thesis, August 1997; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc278947/: accessed August 15, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .