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in creator/contributor: "Lawrence Berkeley National Laboratory. Life Sciences Division."  
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Quantitive DNA Fiber Mapping

Description: Several hybridization-based methods used to delineate single copy or repeated DNA sequences in larger genomic intervals take advantage of the increased resolution and sensitivity of free chromatin, i.e., chromatin released from interphase cell nuclei. Quantitative DNA fiber mapping (QDFM) differs from the majority of these methods in that it applies FISH to purified, clonal DNA molecules which have been bound with at least one end to a solid substrate. The DNA molecules are then stretched by th… more
Date: January 28, 2008
Creator: Lu, Chun-Mei; Wang, Mei; Greulich-Bode, Karin M.; Weier, Jingly F. & Weier, Heinz-Ulli G.
Item Type: Refine your search to only Book
Partner: UNT Libraries Government Documents Department
open access

AmiGO: online access to ontology and annotation data

Description: AmiGO is a web application that allows users to query, browse, and visualize ontologies and related gene product annotation (association) data. AmiGO can be used online at the Gene Ontology (GO) website to access the data provided by the GO Consortium; it can also be downloaded and installed to browse local ontologies and annotations. AmiGO is free open source software developed and maintained by the GO Consortium.
Date: January 15, 2009
Creator: Carbon, Seth; Ireland, Amelia; Mungall, Christopher J.; Shu, ShengQiang; Marshall, Brad & Lewis, Suzanna
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Multidimensional profiling of cell surface proteins and nuclear markers

Description: Cell membrane proteins play an important role in tissue architecture and cell-cell communication. We hypothesize that segmentation and multidimensional characterization of the distribution of cell membrane proteins, on a cell-by-cell basis, enable improved classification of treatment groups and identify important characteristics that can otherwise be hidden. We have developed a series of computational steps to (i) delineate cell membrane protein signals and associate them with a specific nucleu… more
Date: January 30, 2009
Creator: Han, Ju; Chang, Hang; Andarawewa, Kumari; Yaswen, Paul; Helen Barcellos-Hoff, Mary & Parvin, Bahram
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

The Metabolism of Curium in the RAT

Description: The heaviest of the known elements is curium, which was recently discovered by Seaborg and his associates. This new element can be produced by the alpha particle transmutation of plutonium by the following reaction: {sub 94}Pu{sup 239} + {sub 2}He{sup 4} {yields} {sub 96}Cm{sup 242} + {sub 0}N{sup 1} This isotope of curium is radioactive and decays by the emission of an alpha particle to form plutonium 238 which, in turn, is also radioactive. Curium 242 has a half-life of 150 days, and its radi… more
Date: January 15, 1948
Creator: Hamilton, J.; Scott, K. & Axelrod, D.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Basal-Subtype and MEK-Pl3K Feedback Signaling Determine Susceptibility of Breast Cancer Cells to MEK Inhibition

Description: Specific inhibitors of MEK have been developed that efficiently inhibit the oncogenic RAF-MEK-ERK pathway. We employed a systems-based approach to identify breast cancer subtypes particularly susceptible to MEK inhibitors and to understand molecular mechanisms conferring resistance to such compounds. Basal-type breast cancer cells were found to be particularly susceptible to growth-inhibition by small-molecule MEK inhibitors. Activation of the PI3 kinase pathway in response to MEK inhibition th… more
Date: January 23, 2009
Creator: Mirzoeva, Olga K.; Das, Debopriya; Heiser, Laura M.; Bhattacharya, Sanchita; Siwak, Doris; Gendelman, Rina et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

DNA Probe Pooling for Rapid Delineation of Chromosomal Breakpoints

Description: Structural chromosome aberrations are hallmarks of many human genetic diseases. The precise mapping of translocation breakpoints in tumors is important for identification of genes with altered levels of expression, prediction of tumor progression, therapy response, or length of disease-free survival as well as the preparation of probes for detection of tumor cells in peripheral blood. Similarly, in vitro fertilization (IVF) and preimplantation genetic diagnosis (PGD) for carriers of balanced, r… more
Date: January 30, 2009
Creator: Lu, Chun-Mei; Kwan, Johnson; Baumgartner, Adolf; Weier, Jingly F.; Wang, Mei; Escudero, Tomas et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

DNA repair efficiency in germ cells and early mouse embryos and consequences for radiation-induced transgenerational genomic damage

Description: Exposure to ionizing radiation and other environmental agents can affect the genomic integrity of germ cells and induce adverse health effects in the progeny. Efficient DNA repair during gametogenesis and the early embryonic cycles after fertilization is critical for preventing transmission of DNA damage to the progeny and relies on maternal factors stored in the egg before fertilization. The ability of the maternal repair machinery to repair DNA damage in both parental genomes in the fertilizi… more
Date: January 18, 2009
Creator: Marchetti, Francesco & Wyrobek, Andrew J.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Single cell analysis: the new frontier in 'Omics'

Description: Cellular heterogeneity arising from stochastic expression of genes, proteins, and metabolites is a fundamental principle of cell biology, but single cell analysis has been beyond the capabilities of 'Omics' technologies. This is rapidly changing with the recent examples of single cell genomics, transcriptomics, proteomics, and metabolomics. The rate of change is expected to accelerate owing to emerging technologies that range from micro/nanofluidics to microfabricated interfaces for mass spectr… more
Date: January 14, 2010
Creator: Wang, Daojing & Bodovitz, Steven
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Two distinct phases of apoptosis in mammary gland involution: proteinase-independent and -dependent pathways

Description: Postlactational involution of the mammary gland is characterized by two distinct physiological events: apoptosis of the secretory, epithelial cells undergoing programmed cell death, and proteolytic degradation of the mammary gland basement membrane. We examined the spatial and temporal patterns of apoptotic cells in relation to those of proteinases during involution of the BALB/c mouse mammary gland. Apoptosis was almost absent during lactation but became evident at day 2 of involution, when {b… more
Date: January 1, 1996
Creator: Lund, Leif R; Romer, John; Thomasset, Nicole; Solberg, Helene; Pyke, Charles; Bissell, Mina J et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Imaging nanoscale magnetic structures with polarized soft x-ray photons

Description: Imaging nanoscale magnetic structures and their fast dynamics is scientifically interesting and technologically of highest relevance. The combination of circularly polarized soft X-ray photons which provide a strong X-ray magnetic circular dichroism effect at characteristic X-ray absorption edges, with a high resolution soft X-ray microscope utilizing Fresnel zone plate optics allows to study in a unique way the stochastical behavior in the magnetization reversal process of thin films and the u… more
Date: January 18, 2010
Creator: Fischer, P. & Im, M.-Y.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

A Novel Function for the nm23-Hl Gene: Overexpression in Human Breast Carcinoma Cells Leads to the Formation of Basement Membrane and Growth Arrest

Description: We have developed a culture system using reconstituted basement membrane components in which normal human mammary epithelial cells exhibit several aspects of the development and differentiation process, including formation of acinar-like structures, production and basal deposition of basement membrane components, and production and apical secretion of sialomucins. Cell lines and cultures from human breast carcinomas failed to recapitulate this process. The data indicate the importance of cellul… more
Date: January 1, 1994
Creator: Howlett, Anthony R; Petersen, Ole W; Steeg, Patricia S & Bissell, Mina J
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Simulating Scintillator Light Collection Using Measured Optical Reflectance

Description: To accurately predict the light collection from a scintillating crystal through Monte Carlo simulations, it is crucial to know the angular distribution from the surface reflectance. Current Monte Carlo codes allow the user to set the optical reflectance to a linear combination of backscatter spike, specular spike, specular lobe, and Lambertian reflections. However, not all light distributions can be expressed in this way. In addition, the user seldom has the detailed knowledge about the surface… more
Date: January 28, 2010
Creator: Janecek, Martin & Moses, William
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Plasma membrane reorganization induced by tumor promoters in an epithelial cell line

Description: The effects of phorbol ester tumor promoters on the lateral diffusion in plasma membrane lipid environments were examined by the technique of fluorescence recovery after photobleaching. To this end, the probe collarein, a fluorescent lipid analog that has the property of exclusive localization in the plasma membrane, was synthesized. Measured decreases in three parameters [percentage of fluorescence bleached (30%), percentage of recovery (52%), and half-time for recovery (52%)] connoted the app… more
Date: January 1, 1984
Creator: PACKARD, BEVERLY S.; SAXTON, MICHAEL J.; BISSELL, MINA J. & KLEIN, MELVIN P.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Apical polarity in three-dimensional culture systems: where to now?

Description: Delineation of the mechanisms that establish and maintain the polarity of epithelial tissues is essential to understanding morphogenesis, tissue specificity and cancer. Three-dimensional culture assays provide a useful platform for dissecting these processes but, as discussed in a recent study in BMC Biology on the culture of mammary gland epithelial cells, multiple parameters that influence the model must be taken into account.
Date: January 21, 2010
Creator: Inman, J. L. & Bissell, Mina
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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