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in subjects: "Mice -- Nervous system."

open access

Investigations of neuronal network responses to electrical stimulation in murine spinal cultures.

Spontaneous activity in neuronal networks in vitro is common and has been well documented. However, alteration of spontaneous activity in such networks via conditioning electrical stimulation has received much less experimental attention. Two different patterns of electrical stimulation were used to enhance or depress the level of spontaneous activity in spinal cord cultures. High-frequency stimulation (HFS), a method routinely shown to increase the efficacy of synaptic transmission, was employ… more

Date: December 2001

Creator: Sparks, Christopher A.

Item Type: Thesis or Dissertation

Partner: UNT Libraries

open access

Functional Neural Toxicity and Endocrine Responses in Mice Following Naphthalene Exposure

Polycyclic aromatic hydrocarbons (PAHs) are a well studied and diverse class of environmental toxicants. PAHs act via the aryl hydrocarbon receptor (AhR), and studies have suggested that PAHs may elicit neurological and estrogenic effects. Doses of PAHs between 50 to 150 ppm may elicit neurotoxicity in rodent models. The present study investigated the effects of naphthalene on in vivo steroidogenesis in Swiss Webster male mice, and in vitro neural function of Balb-C/ICR mice frontal cortex ne… more

Date: August 2010

Creator: Colbert, Crystal

Item Type: Thesis or Dissertation

Partner: UNT Libraries

open access

Ubiquitin Expression in the Lumbar Spinal Cord Motoneurons of Postnatal Mice-- an Immunohistochemical Study

Maturation of spinal motoneurons in rodents is characterized by a period of cell loss in the embryo, but researchers have claimed that some cell death occurs postnatally. This form of cell death is called apoptosis and involves active participation of the cell. Apoptotic cells have certain recognizable morphological and molecular features. I have used a monoclonal antibody against ubiquitin, (a putative marker of apoptotic cells), to do immunochemistry on mouse spinal cords at various postnatal… more

Date: December 1994

Creator: Chaube, Sanjay

Item Type: Thesis or Dissertation

Partner: UNT Libraries

open access

In Vitro Studies of Nuclear Changes in Mammalian CNS Neurons Subjected to Rapid Acceleration Impact Injury

An in vitro model of Rapid Acceleration Impact (RAI) Injury was used to study the effects of multiple impact (220 g/impact, 3-5 sec intervals) trauma on cultures of murine CNS cells. Investigations with spinal cord cultures showed that 1) multiple impacts delivered tangential to the plane of cell growth caused neuronal death (12% after 3 impacts to 46% after 10 impacts); 2) multiple impacts delivered normal to the plane of cell growth were much less effective (8% dead after 10 impacts); 3) most… more

Date: May 1995

Creator: Wolf, Amy

Item Type: Thesis or Dissertation

Partner: UNT Libraries

open access

Effects of 5-hydroxytryptamine on Mouse Lumbar Motor Activity During Postnatal Development

The lumbar motor activity in isolated spinal cords of 72 postnatal Balb/C mice aged 2, 5, 10 and 21 days (PN2-21) was electroneurographically recorded (ENG) via bilateral ventral roots following treatment with three different concentrations (25, 100 and 200 pM) of the neurotransmitter, 5-hydroxytryptamine (5-HT), i.e., serotonin, to determine its effects on spinal pattern generation.

Date: December 1998

Creator: Lowe-Chatham, Janice E. (Janice Elaine)

Item Type: Thesis or Dissertation

Partner: UNT Libraries

open access

Optimization of Cell Culture Procedures for Growing Neural Networks on Microelectrode Arrays

This thesis describes the development of an optimized method for culturing dissociated, monolayer neuronal networks from murine frontal cortex and midbrain. It is presented as a guidebook for use by cell culture specialists and laboratory personnel who require updated and complete procedures for use with microelectrode array (MEA) recording technology. Specific cell culture protocols, contamination prevention and control, as well common problems encountered within the cell culture facility, are… more

Date: December 2007

Creator: Santa Maria, Cara L.

Item Type: Thesis or Dissertation

Partner: UNT Libraries

open access

Mutation of Polaris, an Intraflagellar Transport Protein, Shortens Neuronal Cilia

Primary cilia are non-motile organelles having 9+0 microtubules that project from the basal body of the cell. While the main purpose of motile cilia in mammalian cells is to move fluid or mucus over the cell surface, the purpose of primary cilia has remained elusive for the most part. Primary cilia are shortened in the kidney tubules of Tg737orpk mice, which have polycystic kidney disease due to ciliary defects. The product of the Tg737 gene is polaris, which is directly involved in a microtub… more

Date: August 2005

Creator: Mahato, Deependra

Item Type: Thesis or Dissertation

Partner: UNT Libraries

open access

Mouse cortical cholinergic neurons: Ontogeny of phenotypes in vivo and in vitro.

The development of cholinergic neurons in mouse frontal cortex was studied both in vivo and in vitro by immunocytochemistry with an antibody to choline acetyltransferase (ChAT), the enzyme responsible for acetylcholine synthesis. While cortical cholinergic neurons have previously been characterized in rat cortex, up until very recently, intrinsic cortical cholinergic neurons were considered to be absent in mouse, and little is known about their development or phenotypic characteristics. The pr… more

Date: August 2005

Creator: Coiculescu, Olivia Elena

Item Type: Thesis or Dissertation

Partner: UNT Libraries

open access

Evaluation of Zinc Toxicity Using Neuronal Networks on Microelectrode Arrays: Response Quantification and Entry Pathway Analysis

Murine neuronal networks, derived from embryonic frontal cortex (FC) tissue grown on microelectrode arrays, were used to investigate zinc toxicity at concentrations ranging from 20 to 2000 mM total zinc acetate added to the culture medium. Continual multi-channel recording of spontaneous action potential generation allowed a quantitative analysis of the temporal evolution of network spike activity generation at specific zinc acetate concentrations. Cultures responded with immediate concentratio… more

Date: August 2007

Creator: Parviz, Maryam

Item Type: Thesis or Dissertation

Partner: UNT Libraries

Determination of Dissociation Constants for GABAA Receptor Antagonists using Spontaneously Active Neuronal Networks in vitro

Changes in spontaneous spike activities recorded from murine frontal cortex networks grown on substrate-integrated microelectrodes were used to determine the dissociation constant (KB) of three GABAA antagonists. Neuronal networks were treated with fixed concentrations of GABAA antagonists and titrated with muscimol, a GABAA receptor agonist. Muscimol decreased spike activity in a concentration dependent manner with full efficacy (100% spike inhibition) and a 50% inhibitory concentration (IC50)… more

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Date: December 2005

Creator: Oli-Rijal, Sabnam

Item Type: Thesis or Dissertation

Partner: UNT Libraries

open access

Roles of Primary Cilia in the Oligodendrocyte Lineage

Primary cilia are nonmotile, hair-shaped organelles that extend from the basal body in the centrosome. The present study is the first investigation of this organelle in the oligodendrocyte lineage in vivo. I used immunohistochemical approaches in normal and cilia-deficient mutant mice to study cilia in relation to oligodendrogenesis and myelination. Primary cilia immunoreactive for Arl13b and ACIII were commonly present in NG2+ oligodendrocyte progenitor cells (OPCs), in which cilia-associated … more

Date: December 2018

Creator: Subedi, Ashok

Item Type: Thesis or Dissertation

Partner: UNT Libraries