Optimization of Cell Culture Procedures for Growing Neural Networks on Microelectrode Arrays

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Description

This thesis describes the development of an optimized method for culturing dissociated, monolayer neuronal networks from murine frontal cortex and midbrain. It is presented as a guidebook for use by cell culture specialists and laboratory personnel who require updated and complete procedures for use with microelectrode array (MEA) recording technology. Specific cell culture protocols, contamination prevention and control, as well common problems encountered within the cell culture facility, are discussed. This volume offers value and utility to the rapidly expanding fields of MEA recording and neuronal cell culture. Due to increasing interest in determining the mechanisms underlying Parkinson's disease, the ... continued below

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Santa Maria, Cara L. December 2007.

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This thesis is part of the collection entitled: UNT Theses and Dissertations and was provided by UNT Libraries to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 2878 times , with 9 in the last month . More information about this thesis can be viewed below.

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  • Santa Maria, Cara L.

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Description

This thesis describes the development of an optimized method for culturing dissociated, monolayer neuronal networks from murine frontal cortex and midbrain. It is presented as a guidebook for use by cell culture specialists and laboratory personnel who require updated and complete procedures for use with microelectrode array (MEA) recording technology. Specific cell culture protocols, contamination prevention and control, as well common problems encountered within the cell culture facility, are discussed. This volume offers value and utility to the rapidly expanding fields of MEA recording and neuronal cell culture. Due to increasing interest in determining the mechanisms underlying Parkinson's disease, the newly developed procedures for mesencephalon isolation and culture on MEAs are an important research contribution.

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UNT Theses and Dissertations

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  • December 2007

Added to The UNT Digital Library

  • May 2, 2008, 3:18 p.m.

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  • Dec. 15, 2008, 12:48 p.m.

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Citations, Rights, Re-Use

Santa Maria, Cara L. Optimization of Cell Culture Procedures for Growing Neural Networks on Microelectrode Arrays, thesis, December 2007; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc5126/: accessed November 22, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .