A new method of collecting light scattering from a liquid flow cytometer has been proposed; this apparatus is named the Mini-Flo flow cytometer. The Mini-Flo uses a high numerical aperture collection immersed in the flow stream. The collector consists of a conically tipped fiber optic pipe and terminating optical detector. This study was performed to improve the signal/noise ration and optimize the Mini-Flo`s performance for HIV blood detection applications. Experiments were performed to gauge the effects of Raman scattering, lens/filter fluorescence, and fiber optic fluorescence on the Mini-Flo`s performance and signal/noise ratio. Results indicated that the fiber optic was a …
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Lawrence Livermore National Lab., CA (United States)
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California
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A new method of collecting light scattering from a liquid flow cytometer has been proposed; this apparatus is named the Mini-Flo flow cytometer. The Mini-Flo uses a high numerical aperture collection immersed in the flow stream. The collector consists of a conically tipped fiber optic pipe and terminating optical detector. This study was performed to improve the signal/noise ration and optimize the Mini-Flo`s performance for HIV blood detection applications. Experiments were performed to gauge the effects of Raman scattering, lens/filter fluorescence, and fiber optic fluorescence on the Mini-Flo`s performance and signal/noise ratio. Results indicated that the fiber optic was a major source of fluorescence noise and reducing its length from 33 cm to 10 cm increased the signal noise ratio from 8 to 75. Therefore, one of the key issues in optimizing the Mini-Flo`s performance is a redesign of the holding structure such that the fiber optic length is minimized. Further improvements of the Mini-Flo`s performance can be achieved by studying the polish of the fiber optic, the flow over the fiber optics`s conical tip, and the optimal particle rates.
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