Optimization of the Mini-Flo flow cytometer

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A new method of collecting light scattering from a liquid flow cytometer has been proposed; this apparatus is named the Mini-Flo flow cytometer. The Mini-Flo uses a high numerical aperture collection immersed in the flow stream. The collector consists of a conically tipped fiber optic pipe and terminating optical detector. This study was performed to improve the signal/noise ration and optimize the Mini-Flo`s performance for HIV blood detection applications. Experiments were performed to gauge the effects of Raman scattering, lens/filter fluorescence, and fiber optic fluorescence on the Mini-Flo`s performance and signal/noise ratio. Results indicated that the fiber optic was a ... continued below

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69 p.

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Venkatesh, M. June 1, 1996.

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Description

A new method of collecting light scattering from a liquid flow cytometer has been proposed; this apparatus is named the Mini-Flo flow cytometer. The Mini-Flo uses a high numerical aperture collection immersed in the flow stream. The collector consists of a conically tipped fiber optic pipe and terminating optical detector. This study was performed to improve the signal/noise ration and optimize the Mini-Flo`s performance for HIV blood detection applications. Experiments were performed to gauge the effects of Raman scattering, lens/filter fluorescence, and fiber optic fluorescence on the Mini-Flo`s performance and signal/noise ratio. Results indicated that the fiber optic was a major source of fluorescence noise and reducing its length from 33 cm to 10 cm increased the signal noise ratio from 8 to 75. Therefore, one of the key issues in optimizing the Mini-Flo`s performance is a redesign of the holding structure such that the fiber optic length is minimized. Further improvements of the Mini-Flo`s performance can be achieved by studying the polish of the fiber optic, the flow over the fiber optics`s conical tip, and the optimal particle rates.

Physical Description

69 p.

Notes

OSTI as DE96050499

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  • Other Information: TH: Thesis (M.S.)

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  • Other: DE96050499
  • Report No.: UCRL-ID--124076
  • Grant Number: W-7405-ENG-48
  • Office of Scientific & Technical Information Report Number: 388136
  • Archival Resource Key: ark:/67531/metadc676536

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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  • June 1, 1996

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  • July 25, 2015, 2:20 a.m.

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  • Feb. 19, 2016, 8:30 p.m.

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Venkatesh, M. Optimization of the Mini-Flo flow cytometer, thesis or dissertation, June 1, 1996; California. (digital.library.unt.edu/ark:/67531/metadc676536/: accessed December 18, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.