The stonefly genus Isogenoides is revised following a holomorphological approach utilizing traditional morphology and behavioral lines of evidence. Species keys are provided for all life stages. One species, I. krumholzi (Ricker) is considered a synonym of I. doratus (Frison). Detailed species descriptions are provided for males, females, nymphs and ova. Distributions are updated utilizing all known published accounts and materials examined. The vibrational communication (drumming) behavior is reported for males and all but one species for females. The signals were species-specific and ranged in complexity from ancestral sequenced duets to derived grouped exchanges. I. olivaceus is least specialized, having mostly sequenced duets, and I. zionensis most specialized, displaying ancestral sequenced, derived grouped and complex derived exchanges containing both sequenced and grouped elements. Laboratory egg incubation experiments over a 2-4 year period show that Isogenoides has a great capacity for extended, sometimes-asynchronous diapause and hatching. The eggs of six species were incubated at a single, ca. simulated San Miguel River, Colorado, seasonal temperature regime. Direct hatch within two weeks occurred only for I. zionensis (Leopard Creek, Colorado) with small numbers hatching again after one, two, and four years. Eggs of I. doratus and I. varians hatched after an over summer, 3-5 month diapause, and I. varians again in August the next year. Populations of I. colubrinus, I. elongatus, I. frontalis and San Miguel River, I. zionensis began hatching after a 9-11 month diapause and again during spring-summer temperatures in 2nd through 4th years. I. zionensis in the San Miguel River, Colorado, exhibited a semivoltine life cycle over the two-year study period. Adults emerged in June-July when stream temperature reached ca. 11-17°C. Reared females at Quartz Creek, Pitkin, Colorado, deposited up to three egg batches. Mean fecundity of females was 691 egg/female. Oviposition in the field was observed and described. Nymphal growth was exponential from June to December, followed by slow growth until April, then declined until emergence. The greatest growth occurred between July and December as stream temperature decreased to minimum and maximum size was obtained in March while stream temperature began to increase.
