Fatty Acid Amide Hydrolases and Chemical Communication in Plants Page: 1

                                
Data Management Plan
Our data management plan aims to keep data secure, accessible to all project personnel and
eventually accessible to the public so that all results can be easily replicated and consistent with
open science initiatives. All of the data collected for this research project will be stored in a
systematic manner. Data collection, title, location, and procedures are routinely recorded by hand
within a standard lab-owned notebook and archived within the PI and Co-Ps research groups. At
Noble, documentation of results and project activities is through the LabArchives Electronic Lab
Notebook (ELN). The ELN utilizes Secure Socket Layer encryption, and offers versioning and
time stamped records. All digital data and documents are tagged with the date and initials of
collector for ease of cataloguing. Computer-based, digital data are backed-up locally and stored
on a shared drive managed by the College of Sciences.Our groups analyze confocal image and
mass spectral data using commercially available software. Raw data files are stored for potential
future retrieval, data mining and processing. These data are accessible initially only by the Pis
and the researchers to protect the integrity of the data, the commercial software license
agreements, and potential intellectual property developed from the research. However, after
publication of the relevant data, associated raw files, processed data, spreadsheets, or other
metadata not included in supplemental files of manuscripts, will be transferred to the UNT Digital
Library (below) where it will be accessible by the public. Hard copy data are also saved within
the lab to support written theses and dissertations and regularly submitted progress reports to
NSF.
Types of Data
For this project, microbiome sequencing data are initially stored as fastq files (.fq), which are text
files containing the nucleotide sequences and the quality score of each base. After processing
and alignment, the data is converted and stored as tab-separated files known as SAM files
(Sequence Alignment/Map) or as BAM files (Binary Alignment/Map), which correspond to
compressed versions of the SAM files. Additional DNA sequences encoding FAAH sequences
will be collected, and catalogued with reference to functional activity assays. Some protein
homology modeling data will be preserved as .pdb files. The majority of the quantitative metabolite
data will be stored in ".csv" files, which are widely used for statistical software, such as but not
limited to MetaboAnalyst. Some MS data will be stored as ".raw" files because these formats are
standard and readable by the majority of free and licensed software. Digital records and
associated metadata of phenotype characterization will be collected. Metadata associated with
the experimental data collected from the instruments will also be preserved from this project.
Storage for Access and Retention
Data will be stored on the backup media and in the Pi's research group for a minimum of five
years after the conclusion of the award. In addition, we will utilize the facilities provided by the
UNT Data Repository (http://digital.library.unt.edu/datarepository), a part of the UNT Digital
Library (http://digital.library.unt.edu), which is committed to long-term access and stewardship of
publicly available research outputs. Raw and derived datasets and information objects produced
will be retained for at least 5 years after the end of the project, and may be available longer
through the UNT Digital Library. The UNT libraries request compensation for storage at a cost of
$100 per TB per year for 5 years, and we anticipate that 3TB of storage ($300.00) will be more
than adequate for our project. The project team will work with the UNT Libraries in appraising the
information objects for long-term preservation, and to determine appropriate embargo periods, if
any, for the individual digital resources. For data sources that are embargoed for some period of
time, the metadata records will be available to allow discovery of the resources. All project-related
materials, such as technical reports, presentations, and publications, will be made long-term

1