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Oncogene mRNA Imaging with Radionuclide-PNA-Peptides

Description: New cancer gene hybridization probes to carry radionuclides were made. Noninvasive technetium-99m gamma imaging of CCND1 cancer gene activity in human breast cancer tumors in mice was demonstrated, followed by noninvasive technetium-99m imaging of MYC cancer gene activity. Noninvasive imaging of CCND1 cancer gene activity in human breast cancer tumors in mice was demonstrated with a positron-emitting copper-64 probe, followed by noninvasive positron imaging of IRS1 cancer gene activity.
Date: March 19, 2008
Creator: Wickstrom, Eric
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

DNA-DNA Hybridization of Methane Oxidizing Bacteria

Description: Bacteria classified in the family Methylomonadaceae must derive their carbon from one-carbon compounds. They are characterized by the possession of internal membranes of two types. Type I membranes are layered and fill the middle of the cells while type II membranes form concentric layers around the periphery of the cells. Also, there are two metabolic pathways by which the methylobacteria assimilate one-carbon compounds. Further evidence of this dichotomy was sought by DNA-DNA saturation hybri… more
Date: December 1976
Creator: Ackerson, Jill W.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Microarray-based whole-genome hybridization as a tool for determining procaryotic species relatedness

Description: The definition and delineation of microbial species are of great importance and challenge due to the extent of evolution and diversity. Whole-genome DNA-DNA hybridization is the cornerstone for defining procaryotic species relatedness, but obtaining pairwise DNA-DNA reassociation values for a comprehensive phylogenetic analysis of procaryotes is tedious and time consuming. A previously described microarray format containing whole-genomic DNA (the community genome array or CGA) was rigorously ev… more
Date: January 15, 2008
Creator: Wu, L.; Liu, X.; Fields, M.W.; Thompson, D.K.; Bagwell, C.E.; Tiedje, J. M. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Final Report on ``Theories of Strong Electron Correlations in Molecules and Solids’’ - DE-FG02-97ER45640

Description: The PI led theoretical studies of correlated hybridization in transition metal complexes, compounds, and molecules, and of electron transport in DNA associated with nanoelectronic conformations attached to gold electrodes and in the presence of DNA repair proteins.
Date: April 15, 2013
Creator: Cox, Daniel L.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Melting of Xenon to 80 GPa, p-d hybridization, and an ISRO liquid

Description: Measurements made in a laser heated diamond-anvil cell are reported that extend the melting curve of Xe to 80 GPa and 3350 K. The steep lowering of the melting slope (dT/dP) that occurs near 17 GPa and 2750 K results from the hybridization of the p-like valence and d-like conduction states with the formation of clusters in the liquid having Icosahedral Short-Range Order (ISRO).
Date: July 26, 2005
Creator: Ross, M; Boehler, R & Soderlind, P
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Phylogenetic Relationships Among Subsurface Microorganisms

Description: This project involves the development of group specific 16S ribosomal RNA-targeted oligonucleotide hybridization probes for the rapid detection of specific types of subsurface organisms (e.g., groups of microbes that share certain physiological traits). Major accomplishments for the period of 6/91 to 12/1/91 are described. Nine new probes have been synthesized on the basis of published 16S rRNA sequence data from the Ribosomal Database Project. We have initiated rapid screening of many of the s… more
Date: January 1, 1991
Creator: Nierzwicki-Bauer, S. A.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Molecular cytogenetics using fluorescence in situ hybridization

Description: Fluorescence in situ hybridization (FISH) with chromosome-specific probes enables several new areas of cytogenetic investigation by allowing visual determination of the presence and normality of specific genetic sequences in single metaphase or interphase cells. in this approach, termed molecular cytogenetics, the genetic loci to be analyzed are made microscopically visible in single cells using in situ hybridization with nucleic acid probes specific to these loci. To accomplish this, the DNA i… more
Date: December 7, 1990
Creator: Gray, J.W.; Kuo, Wen-Lin; Lucas, J.; Pinkel, D.; Weier, H-U. & Yu, Loh-Chung.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

[Melting in Adsorbed Films]

Description: Over the past several years we have been developing a new approach to cloning large fragments of mammalian DNA in E. coli. which will permit detailed analysis of complex genomes. In January 1992 we began construction of an arrayed total human genomic library prepared in our BAC vector. Our goal is to create a 4-5X library which will be accessible for screening both by colony hybridization and by PCR. Our efforts in 1992 have been focused on expanding this library, characterizing specific clones… more
Date: January 1, 1992
Creator: Simon, M. I.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Oncogene amplification detected by in situ hybridization in radiation induced rat skin tumors. [C-myc:a3]

Description: Oncogene activation may play an important role in radiation induced carcinogenesis. C-myc oncogene amplification was detected by in situ hybridization in radiation-induced rat skin tumors, including squamous and basal cell carcinomas. In situ hybridization was performed with a biotinylated human c-myc third exon probe, visualized with an avidin-biotinylated alkaline phosphate detection system. No c-myc oncogene amplification was detected in normal rat skin at very early times after exposure to … more
Date: February 1, 1991
Creator: Jin., Yi
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Highly specific electronic signal transduction mediated by DNA/metal self-assembly.

Description: Highly specific interactions between DNA could potentially be amplified if the DNA interactions were utilized to assemble large scale parts. Fluidic assembly of microsystem parts has the potential for rapid and accurate placement of otherwise difficult to handle pieces. Ideally, each part would have a different chemical interaction that allowed it to interact with the substrate only in specific areas. One easy way to obtain a multiple chemical permutations is to use synthetic DNA oligomers. Si … more
Date: November 1, 2003
Creator: Dentinger, Paul M. & Pathak, Srikant
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Correlations between Optical, Chemical and Physical Properties of Biomass Burn Aerosols

Description: Aerosols generated from burning different plant fuels were characterized to determine relationships between chemical, optical and physical properties. Single scattering albedo ({omega}) and Angstrom absorption coefficients ({alpha}{sub ap}) were measured using a photoacoustic technique combined with a reciprocal nephelometer. Carbon-to-oxygen atomic ratios, sp{sup 2} hybridization, elemental composition and morphology of individual particles were measured using scanning transmission X-ray micro… more
Date: January 29, 2008
Creator: Hopkins, Rebecca J.; Lewis, K.; Desyaterik, Yury; Wang, Z.; Tivanski, Alexei V.; Arnott, W. P. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Effects of doping on hybridization gapped materials

Description: Doping studies are presented on three materials exhibiting hybridization gaps: Ce{sub 3}Bi{sub 4}Pt{sub 3}, U{sub 3}Sb{sub 4}Pt{sub 3}, and CeRhSb. In the case of trivalent La, Y, or Lu substituting for Ce or U, there is a suppression of the low temperature gap and an increase in the electronic specific heat, {gamma}. In the case of tetravalent Th substitutions for U there is no change in {gamma} and in the case of tetravalent Zr substitution for Ce in CeRhSb, there is an enhanced semiconductor… more
Date: June 5, 1991
Creator: Canfield, P. C.; Thompson, J. D.; Hundley, M. F.; Lacerda, A. & Fisk, Z.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

An improved method for producing radiation hybrids applied to human chromosome 19

Description: At the initiation of the grant we had just produced radiation hybrids from a monochromosomal microcell hybrid containing human chromosome 19 as its only human component. Radiation hybrids were produced using doses of radiation ranging from 1000--8000 rads. Lethally irradiated cells were then fused to hamster recipients (CHTG49) and selected for growth in histidinol. Approximately 240 clones were isolated and 75 clones were expanded for the isolation of DNA. This report describes in situ hybridi… more
Date: January 1, 1992
Creator: Jackson, C.L.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Surface Bonding Effects in Compound Semiconductor Nanoparticles: II

Description: Small nanoparticles have a large proportion of their atoms either at or near the surface, and those in clusters are essentially all on the surface. As a consequence, the details of the surface structure are of paramount importance in governing the overall stability of the particle. Just as with bulk materials, factors that determine this stability include “bulk” structure, surface reconstruction, charge balance and hybridization, ionicity, strain, stoichiometry, and the presence of adsorbates. … more
Date: July 1, 2008
Creator: Farrell, Helen H.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

SBH and the integration of complementary approaches in the mapping, sequencing, and understanding of complex genomes

Description: A variant of sequencing by hybridization (SBH) is being developed with a potential to inexpensively determine up to 100 million base pairs per year. The method comprises (1) arraying short clones in 864-well plates; (2) growth of the M13 clones or PCR of the inserts; (3) automated spotting of DNAs by corresponding pin-arrays; (4) hybridization of dotted samples with 200-3000 [sup 32]P- or [sup 33]P-labeled 6- to 8-mer probes; and (5) scoring hybridization signals using storage phosphor plates. … more
Date: January 1, 1992
Creator: Drmanac, R.; Drmanac, S.; Labat, I.; Vicentic, A.; Gemmell, A.; Stavropoulos, N. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Validation of DNA probes for molecular cytogenetics by mapping onto immobilized circular DNA

Description: Fluorescence in situ hybridization (FISH) is a sensitive and rapid procedure to detect gene rearrangements in tumor cells using non-isotopically labeled DNA probes. Large insert recombinant DNA clones such as bacterial artificial chromosome (BAC) or P1/PAC clones have established themselves in recent years as preferred starting material for probe preparations due to their low rates of chimerism and ease of use. However, when developing probes for the quantitative analysis of rearrangements invo… more
Date: December 16, 2008
Creator: Greulich-Bode, Karin; Wang, Mei; Rhein, Andreas; Weier, Jingly & Weier, Heinz-Ulli
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Multiscale integration schemes for jump-diffusion systems

Description: We study a two-time-scale system of jump-diffusion stochastic differential equations. We analyze a class of multiscale integration methods for these systems, which, in the spirit of [1], consist of a hybridization between a standard solver for the slow components and short runs for the fast dynamics, which are used to estimate the effect that the fast components have on the slow ones. We obtain explicit bounds for the discrepancy between the results of the multiscale integration method and the … more
Date: December 9, 2008
Creator: Givon, D. & Kevrekidis, I.G.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Human cDNA mapping using fluorescence in situ hybridization. Progress report, April 1, 1992--December 31, 1992

Description: Genetic mapping is approached using the techniques of high resolution fluorescence in situ hybridization (FISH). This technology and the results of its application are designed to rapidly generate whole genome as tool box of expressed sequence to speed the identification of human disease genes. The results of this study are intended to dovetail with and to link the results of existing technologies for creating backbone YAC and genetic maps. In the first eight months, this approach generated 60-… more
Date: March 4, 1993
Creator: Korenberg, J. R.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Final Report: Sequence Landscapes, March 1, 1998 - June 30, 1999

Description: Sequence Landscapes are a graphical display of the word frequencies from a database (DB) for every word of every length in a target sequence (TS). If the TS and the DB are the same sequence this is a convenient method to detect all of the repeated sequences, of any length. However, we have been exploring the use of this approach for classifying regions of DNA sequence into functional domains, such as exons, introns, promoters, etc. Using DB from each class, the landscapes can be used to derive … more
Date: June 30, 1999
Creator: Stormo, Gary D.; Levy, Samuel & Li, Fugen
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Chromosomal mosaicism in mouse two-cell embryos after paternal exposure to acrylamide

Description: Chromosomal mosaicism in human preimplantation embryos is a common cause ofspontaneous abortions, however, our knowledge of its etiology is limited. We used multicolor fluorescence in situ hybridization (FISH) painting to investigate whether paternally-transmitted chromosomal aberrations result in mosaicism in mouse 2-cell embryos. Paternal exposure to acrylamide, an important industrial chemical also found in tobacco smoke and generated during the cooking process of starchy foods, produced sig… more
Date: October 14, 2008
Creator: Marchetti, Francesco; Bishop, Jack; Lowe, Xiu & Wyrobek, Andrew J
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Ab-Initio Study on Plutonium Compounds Pu3M (M=Al, Ga, In), PuNp and Elemental Neptunium

Description: Using spin-polarized relativistic density functional theory the electronic and magnetic structures for the plutonium compounds Pu{sub 3}M(M = Al; Ga; In) and PuNp have been investigated. For the first group of compounds the enhanced hybridization between Pu 5f and p-states of alloying element, as it has been found in spin-polarized calculations, is believed to be the main reason for the higher formation energies obtained in such kind of studies in comparison with the non-spin-polarized case. Al… more
Date: September 7, 2005
Creator: Kutepov, A L
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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