Phylogenetic relationships among subsurface microorganisms

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Description

This project involves the development of group specific 16S ribosomal RNA-targeted oligonucleotide hybridization probes for the rapid detection of specific types of subsurface organisms (e.g., groups of microbes that share certain physiological traits). Major accomplishments for the period of 6/91 to 12/1/91 are described. Nine new probes have been synthesized on the basis of published 16S rRNA sequence data from the Ribosomal Database Project. We have initiated rapid screening of many of the subsurface microbial isolates obtained from the P24 borehole at the Savannah River Site. To date, we have screened approximately 50% of the isolates from P24. We have ... continued below

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Pages: (10 p)

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Nierzwicki-Bauer, S.A. January 1, 1991.

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Description

This project involves the development of group specific 16S ribosomal RNA-targeted oligonucleotide hybridization probes for the rapid detection of specific types of subsurface organisms (e.g., groups of microbes that share certain physiological traits). Major accomplishments for the period of 6/91 to 12/1/91 are described. Nine new probes have been synthesized on the basis of published 16S rRNA sequence data from the Ribosomal Database Project. We have initiated rapid screening of many of the subsurface microbial isolates obtained from the P24 borehole at the Savannah River Site. To date, we have screened approximately 50% of the isolates from P24. We have optimized our {und in situ} hybridization technique, and have developed a cell blot hybridization technique to screen 96 samples on a single blot. This is much faster than reading 96 individual slides. Preliminary experiments have been carried out which indicate specific nutrients can be used to amplify rRNA only in those organisms capable of metabolizing those nutrients. 1 tab., 2 figs.

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Pages: (10 p)

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OSTI; NTIS; GPO Dep.

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  • Other: DE92004421
  • Report No.: DOE/ER/60989-2
  • Grant Number: FG02-90ER60989
  • DOI: 10.2172/6106595 | External Link
  • Office of Scientific & Technical Information Report Number: 6106595
  • Archival Resource Key: ark:/67531/metadc1108431

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Office of Scientific & Technical Information Technical Reports

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  • January 1, 1991

Added to The UNT Digital Library

  • Feb. 22, 2018, 7:45 p.m.

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  • March 27, 2018, 2:51 p.m.

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Nierzwicki-Bauer, S.A. Phylogenetic relationships among subsurface microorganisms, report, January 1, 1991; Troy, New York. (digital.library.unt.edu/ark:/67531/metadc1108431/: accessed June 19, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.