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Clonal theory of radiation carcinogenesis

Description: In some cases, usually involving high-LET radiations, the dose response at low doses follows a power function of dose with exponent less than one over a wide dose range. This type of response is of great interest since (a) it implies greater effect per unit dose at progressively smaller doses, and (b) it is not predicted by most models and theories of radiobiology. A theoretical framework is presented for responses having the above characteristics over a dose range extending over a factor of 10… more
Date: January 1, 1982
Creator: Baum, J.W.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Chromosome region-specific libraries for human genome analysis

Description: We have made important progress since the beginning of the current grant year. We have further developed the microdissection and PCR- assisted microcloning techniques using the linker-adaptor method. We have critically evaluated the microdissection libraries constructed by this microtechnology and proved that they are of high quality. We further demonstrated that these microdissection clones are useful in identifying corresponding YAC clones for a thousand-fold expansion of the genomic coverage… more
Date: January 1, 1991
Creator: Kao, Fa-Ten.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Optimizing procedures for a human genome repository

Description: Large numbers of clones will be generated during the Human Genome Project. As each is characterized, subsets will be identified which are useful to the scientific community at large. These subsets are most readily distributed through public repositories. The American Type Culture Collection (ATCC) is experienced in repository operation, but before this project had no history in managing clones and associated information in large batches instead of individually. This project permitted the ATCC t… more
Date: March 1, 1991
Creator: Nierman, W.C.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Aquatic species project report: FY 1991

Description: This report summarizes the progress and research accomplishments of the Aquatic Species Project, which is managed by the National Renewable Energy Laboratory for the US Department of Energy. The project is focused on applying genetic engineering techniques to enhance the lipid, or oil, production of microalgae. Those lipids can be extracted and processed into high-energy liquid fuels such as diesel. Because microalgae require carbon dioxide, a major greenhouse'' gas, as a nutrient, project rese… more
Date: April 1, 1992
Creator: Brown, L.M. (National Renewable Energy Lab., Golden, CO (United States)) & Sprague, S. (USDOE, Washington, DC (United States))
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Molecular biological enhancement of coal biodesulfurization. [Rhodococcus]

Description: The objective of this project is to produce one or more microorganisms capable of removing the organic and inorganic sulfur in coal. The original specific technical objectives of the project were to: Clone and characterize the genes encoding the enzymes of the 4S'' pathway (sulfoxide/sulfone/sulfonate/sulfate) for release of organic sulfur from coal; Return multiple copies of genes to the original host to enhance the biodesulfurization activity of that organism; Transfer this pathway into a fas… more
Date: March 15, 1990
Creator: Litchfield, J. H.; Fry, I.; Wyza, R. E.; Palmer, D. T.; Zupancic, T. J. & Conkle, H. N.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Molecular biological enhancement of coal biodesulfurization. [Rhodococcus, thiobacillus]

Description: The objective of this project is to produce one or more microorganisms capable of removing the organic and inorganic sulfur in coal. The original specific technical objectives of the project were to: clone and characterize the genes encoding the enzymes of the 4S'' pathway (sulfoxide/sulfone/sulfonate/sulfate) for release of organic sulfur from coal; return multiple copies of genes to the original host to enhance the biodesulfurization activity of that organism; transfer this pathway into a fas… more
Date: June 14, 1990
Creator: Litchfield, J. H.; Fry, I.; Wyza, R. E.; Palmer, D. T.; Zupancic, T. J. & Conkle, H. N.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Studies of human mutation rates

Description: The three objectives of the program are: To isolate by the technique of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), proteins of special interest because of the relative mutability of the corresponding gene, establish the identity of the protein, and, for selected proteins, move to a characterization of the corresponding gene; To develop a more efficient approach, based on 2-D PAGE, for the detection of variants in DNA, with special reference to the identification of a variant… more
Date: July 15, 1991
Creator: Neel, J.V.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Molecular biology of Lea genes of higher plants

Description: This report contains our progress to date in determining the function of the D-7 Lea proteins in cotton embryos. We have completely sequenced the D-7 gene and established {ital E. coli} transformants which synthesize reasonable amounts of the D-7 protein. Two-dimensional electrophoresis was required to assay fractions for D-7 protein during purification to homogeneity, since D-7 has no known enzymatic activity, contains no Trp, and little Phe or Tyr, and {ital E. coli} has several proteins of s… more
Date: July 1, 1991
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Characterization of a defective interfering RNA that contains a mosaic of a plant viral genome

Description: Our lab was the first to describe and characterize a defective interfering RNA (DI RNAs or DIs) in association with a small RNA plant virus. The features of the DIs that we discovered in infections of tomato bushy stunt virus were compatible with the properties of DIs identified in many animal virus infections. Animal virologists have generally recognized the importance of studying DIs because they are invaluable tools for identifying cis-acting sequences important in virus multiplication and b… more
Date: January 1, 1991
Creator: Morris, T. J. & Jackson, A. O.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Enzymology of biological nitrogen fixation

Description: Two genes involved in the regulation of nitrogenase activity, draT and draG, were cloned and found to be contiguous on the Azospirillum brasilense chromosome. The nifH gene, encoding dinitrogenase reductase, is near to draT with an intervening gap of 1.9 kb. The organization of these genes in Azospirillum lipoferum and Rhodosprillum rubrum is similar, but nifH and draT are separated by only 400 bp in the organisms. A. brasilense draTG is very similar to draTG in R. rubrum with 91.8% similarity … more
Date: January 1, 1992
Creator: Burris, R.H.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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DOE contractors' workshop: Cellular and molecular aspects of radiation induced DNA damage and repair

Description: For four decades the US Department of Energy and its predecessors have been the lead federal agency in supporting radiation biology research. Over the years emphasis in this program has gradually shifted from dose-effect studies on animals to research on the effects of radiations of various qualities on cells and molecules. Mechanistic studies on the action of radiation at the subcellular level are few in number and there is a need for more research in this area if we are to gain a better under… more
Date: January 1, 1987
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Molecular biological enhancement of coal biodesulfurization. [Rhodococcus]

Description: The objective of this project is to produce one or more microorganisms capable of removing the organic and inorganic sulfur in coal. The original specific technical objectives of the project were to: Clone and characterize the genes encoding the enzymes of the 4S'' pathway (sulfoxide/sulfone/sulfonate/sulfate) for release of organic sulfur from coal; return multiple copies of genes to the original host to enhance the biodesulfurization activity of that organism; transfer this pathway into a fas… more
Date: September 15, 1989
Creator: Litchfield, J. H.; Palmer, D. T.; Zupancic, T. J. & Conkle, H. N. (Battelle, Columbus, OH (United States))
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Characterization of a 1,4-. beta. -D-glucan synthase from Dictyostelium discoideum

Description: Various aspects of research concerning Dictyostelium discoideum are presented. The initial focus of this project was upon: the characterization of potential probes for the cellulose synthase (antibody and nucleic acid), the determination of the cultural induction conditions of cellulose synthesis, the solubilization of the enzyme activity, the development of a non-inhibitory disruption buffer, the generation and isolation of mutant strains deficient in cellulose synthesis, and the development o… more
Date: January 15, 1992
Creator: Blanton, R.L.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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(New hosts and vectors for genome cloning)

Description: The main goal of our project remains the development of new bacterial hosts and vectors for the stable propagation of human DNA clones in E. coli. During the past six months of our current budget period, we have (1) continued to develop new hosts that permit the stable maintenance of unstable features of human DNA, and (2) developed a series of vectors for (a) cloning large DNA inserts, (b) assessing the frequency of human sequences that are lethal to the growth of E. coli, and (c) assessing th… more
Date: January 1, 1991
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Carbon monoxide metabolism by the photosynthetic bacterium Rhodospirillum rubrum

Description: Research continued on carbon monoxide metabolism by Rhodospirillum rubrum. In the past year, progress was made in: (1) the identification and isolation of the physiological electron carrier from monoxide dehydrogenase (CODH) to hydrogenase in R. rubrum; (2) the isolation, sequencing and mutagenesis of the genes encoding the components of the CO oxidation system in R. rubrum, (3) the purification and characterization of the CO-induced hydrogenase activity of R. rubrum; (4) the spectroscopic inve… more
Date: January 1, 1991
Creator: Ludden, P. W. & Roberts, G. P.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Molecular biological enhancement of coal biodesulfurization

Description: The objective of this project is to produce one or more microorganisms capable of the organic and inorganic sulfur in coal. The original specific technical objectives of the project were to: (1) clone and characterize the genes encoding the enzymes of the 4S'' pathway (sulfoxide/sulfone/sulfonate/sulfate) for release of organic sulfur from coal; (2) return multiple copies of genes to the original host to enhance the biodesulfurization activity of that organism; (3) transfer this pathway into a … more
Date: March 13, 1992
Creator: Litchfield, J. H.; Zupancic, T. J.; Kittle Jr., J. D.; Baker, B.; Palmer, D. T.; Traunero, C. G. et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Differential gene expression in C4 plants. Research proposal, February 1, 1982-January 31, 1983. [Pea plants]

Description: The topic of this research proposal is slightly different from that originally outlined. Specifically, instead of characterizing the genes encoding the small subunit of RuBP carboxylase and the chlorophyll a/b binding polypeptide from corn, these genes from pea are being characterized. The above polypeptides represent the major products of cytoplasmic protein synthesis in green leaves. CDNA clones encoding the above polypeptides were isolated and characterized. Both of these cDNA clones have no… more
Date: November 1, 1981
Creator: Cashmore, A. R.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Enzymology of acetone-butanol-isopropanol formation

Description: The long-term goal of the project is to understand the fundamental properties of biological solvent production. Our approach is to elucidate first the molecular properties of solvent-producing enzymes and then to apply to information gained from the enzymological study to investigate control mechanisms for the solvent-producing pathways and the expression of solvent-production genes. Our research primarily involves two strains of Clostridium beijerinckii: C. Beijerinckii NRRL B593 which produce… more
Date: January 1, 1992
Creator: Chen, Jiann-Shin
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Primer on molecular genetics

Description: This report is taken from the April 1992 draft of the DOE Human Genome 1991--1992 Program Report, which is expected to be published in May 1992. The primer is intended to be an introduction to basic principles of molecular genetics pertaining to the genome project. The material contained herein is not final and may be incomplete. Techniques of genetic mapping and DNA sequencing are described.
Date: April 1, 1992
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Cellular energy metabolism

Description: Studies have been carried out on adenylate kinase which is an important enzyme in determining the concentrations of the adenine nucleotides. An efficient method has been developed to clone mutant adenylate kinase genes in E. coli. Site-specific mutagenesis of the wild type gene also has been used to obtain forms of adenylate kinase with altered amino acids. The wild type and mutant forms of adenylate kinase have been overexpressed and large quantities were readily isolated. The kinetic and fluo… more
Date: June 1, 1991
Creator: Glaser, M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Structural Genomics of Minimal Organisms: Pipeline and Results

Description: The initial objective of the Berkeley Structural Genomics Center was to obtain a near complete three-dimensional (3D) structural information of all soluble proteins of two minimal organisms, closely related pathogens Mycoplasma genitalium and M. pneumoniae. The former has fewer than 500 genes and the latter has fewer than 700 genes. A semiautomated structural genomics pipeline was set up from target selection, cloning, expression, purification, and ultimately structural determination. At the ti… more
Date: September 14, 2007
Creator: Kim, Sung-Hou; Shin, Dong-Hae; Kim, Rosalind; Adams, Paul & Chandonia, John-Marc
Item Type: Refine your search to only Book
Partner: UNT Libraries Government Documents Department
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Enzymatic Upgrading of Heavy Crudes via Partial Oxidation or Conversion of PAHs

Description: The objective of this program was to investigate new enzyme-based technologies for upgrading of heavy oils. Enzymes were selected for screening from those capable of conversion of polyaromatic hydrocarbons (PAHs) reported in the literature. Oxidative reactions of PAHs using hydrogen peroxide as an oxidant with conversion to partially oxidized products were used. The enzymes (lignin peroxidase, cytochrome c) were tested in various organic solvents and found to loose activity in pure organic solv… more
Date: July 1, 2002
Creator: Borole, A. P.; Davison, B. H. & Kuritz, T.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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A nanostructure-initiator mass spectrometry-based enzyme activity assay

Description: We describe a Nanostructure-Initiator Mass Spectrometry (NIMS) enzymatic (Nimzyme) assay in which enzyme substrates are immobilized on the mass spectrometry surface by using fluorous-phase interactions. This 'soft' immobilization allows efficient desorption/ionization while also enabling the use of surface-washing steps to reduce signal suppression from complex biological samples, which results from the preferential retention of the tagged products and reactants. The Nimzyme assay is sensitive … more
Date: March 10, 2008
Creator: Siuzdak, Gary; Northen, Trent R.; Lee, Jinq-Chyi; Hoang, Linh; Raymond, Jason; Hwang, Der-Ren et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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