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in subjects: "Cell Flow Systems"

open access

Biomedical and Environmental Research program of the LASL Health Division. Progress report, January--December 1976

Separate abstracts were prepared for 20 sections of the report. (HLW)

Date: July 1, 1977

Creator: Petersen, D.F. & Sullivan, E.M. (comps.)

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

Detailed studies on the application of three fluorescent antibiotics for DNA staining in flow cytometry

The effects of various fixative agents, pH, ionic strength, stain concentration, and magnesium concentration on DNA staining with the fluorescent antibiotics olivomycin, chromomycin A3, and mithramycin were examined with DNA in solution and in mammalian cells.

Date: January 1, 1977

Creator: Crissman, H.A.; Stevenson, A.P.; Orlicky, D.J. & Kissane, R.J.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Cytometry of mammalian sperm

Male germ cells respond dramatically to a variety of insults and are important reproductive dosimeters. Semen analyses are very useful in studies on the effects of drugs, chemicals, and environmental hazards on testicular function, male fertility and heritable germinal mutations. The accessibility of male cells makes them well suited for analytical cytology. We might automate the process of determining sperm morphology but should not do so solely for increased speed. Rather, richer tangible ben… more

Date: October 11, 1983

Creator: Gledhill, B.L.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

New methodlogy for measuring damage to respiratory tract cells using flow-system cell-analysis techniques

Automated flow-system cell-analysis techniques provide a new method for determining damage to respiratory tract cells in humans exposed by inhalation of environmental pollutants such as from the production of synthetic fuels from coal and oil shale. This paper describes results of preliminary experiments designed to develop flow-system instrumentation methods for measuring biological damage to respiratory tract cells using the Syrian hamster as a model. Hamster respiratory tract cell samples we… more

Date: January 1, 1977

Creator: Steinkamp, J. A.; Hansen, K. M.; Wilson, J. S. & Salzman, G. C.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Translocation of particles to the pleural space and tracheobronchial lymph nodes following lung deposition

The translocation of particles from the alveolar compartment to the pleural space and tracheobronchial lymph nodes was investigated in rats over a 30 day period following the intrapulmonary instillation of 4 x 10/sup 8/ polystyrene microspheres (1.9 ..mu..m dia.). In initial studies, approx. =10/sup 4/ particles were found in the pleural space compartment on Days 1, 14 and 30 after particle deposition, and most of these were cell-associated. Subsequent pleural space studies indicated, however, … more

Date: January 1, 1985

Creator: Lehnert, B. E.; Valdez, Y. E. & Hyler, S.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Determination of somatic mutations in human erythrocytes by cytometry

Flow cytometric assays of human erythrocytes labeled with monoclonal antibodies specific for glycophorin A were used to enumerate variant cells that appear in peripheral blood as a result of somatic gene-loss mutations in erythrocyte precursor cells. The assay was performed on erythrocytes from 10 oncology patients who had received at least one treatment from radiation or mutagenic chemotherapy at least 3 weeks before being assayed. The patients were suffering from many different malignancies (… more

Date: June 21, 1985

Creator: Jensen, R.H.; Langlois, R.G. & Bigbee, W.L.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Cytometric analysis of shape and DNA content in mammalian sperm

Male germ cells respond dramatically to a variety of insults and are important reproductive dosimeters. Semen analyses are very useful in studies on the effects of drugs, chemicals, and environmental hazards on testicular function, male fertility and heritable germinal mutations. Sperm were analyzed by flow cytometry and slit-scan flow analysis for injury following the exposure of testes to mutagens. The utility of flow cytometry in genotoxin screening and monitoring of occupational exposure wa… more

Date: October 10, 1983

Creator: Gledhill, B.L.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Correlation of the growth of Chinese hamster V/sub 2/79-171b multicellular spheroids with cytokinetic parameters

The volumes of Chinese hamster V/sub 2/79-171b multicellular spheroids grown in spinner culture were measured and correlated to DNA distribution and cell volume distribution information. Spheroid growth curves were obtained over a 21 day period using both electronic cell volume measurements and microscope micrometer sizing. The spheroids were collected from the aperture tube after electronic sizing, and single cell suspensions were prepared via trypsinization. Saline-washed cells were then stai… more

Date: January 1, 1979

Creator: Johnson, T.S.; Bain, E.; Raju, M.R. & Martin, J.C.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Laser flow cytophotometric light scatter and fluorescence time-of-flight sizing of mammalian cells. [Use of pulse width or pulse rise time measurements]

In laser flow cytophotometry, an increasingly popular technique of analytical cytology, quantitative measurements of interest include cell and nuclear diameters. Electronic circuitry for a new method of time-of-flight cell sizing was developed which measures the time that signal pulses from either fluorescence or light scatter sensors exceed a preset constant fraction of the peak signal amplitude (pulse width) or the time that it takes a signal to rise between constant fractions of the peak sig… more

Date: January 1, 1978

Creator: Leary, J.F.; Todd, P.; Wood, J.C.S. & Jett, J.H.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Flow cytometric measurements of cell surface phenomena

The study of cell surface phenomena by flow cytometry has been pursued by several groups. These studies have produced new insights into a variety of areas of biology. However, the capabilities of flow systems have not been fully exploited. A technique for discriminating between surface and volume fluorescence is proposed along with a method to quantitate cap formation in lymphocytes.

Date: January 1, 1979

Creator: Jett, J.H.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Pacific Northwest Laboratory annual report for 1985 to the DOE Office of Energy Research. Part 4. Physical sciences

Part 4 of the Pacific Northwest Laboratory Annual Report for 1985 to the DOE Office of Energy Research includes those programs funded under the title ''Physical and Technological Research.'' The Field Task Program Studies reports in this document are grouped by budget category and each section is introduced by an abstract that indicates the Field Task Proposal/Agreement reported in that section. These reports only briefly indicate progress made during 1985. The reader should contact the princip… more

Date: February 1, 1986

Creator: Toburen, L. H.

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

Resources for flow and image cytometry

This paper describes resources available to the flow and image cytometry community. I have been asked to limit the discussion to resources available in the United States, so reference to resources exclusively available in Japan, Europe, or Australia are not included. It is not the intention of this paper to include each and every resource available, rather, to describe the types available and give some examples. Included in this manuscript are listings of some of the examples of resources which… more

Date: January 1, 1990

Creator: Cassidy, M.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Cytometric analysis of mammalian sperm for induced morphologic and DNA content errors

Some flow-cytometric and image analysis procedures under development for quantitative analysis of sperm morphology are reviewed. The results of flow-cytometric DNA-content measurements on sperm from radiation exposed mice are also summarized, the results related to the available cytological information, and their potential dosimetric sensitivity discussed. (ACR)

Date: June 27, 1983

Creator: Pinkel, D.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Livermore flow cytometer

A stable, easy to operate, flow cytometer was built for routine use by research personnel in a biology laboratory. A 5 watt argon-ion laser excites the fluorescent dyes in cells flowing through a quartz flow chamber. A f/0.95 TV camera lens collects the fluorescent and scattered light and directs it through color separation filters and a beam splitter onto two photomultiplier tubes. Two 3-axis positioners are used to align the focused laser beam and the flow chamber with the light collection op… more

Date: November 19, 1981

Creator: Peters, D.C.; Dean, P.N. & Pinkel, D.

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

Single particle analysis with a 360/sup 0/ light scattering photometer

Light scattering by single spherical homogeneous particles in the diameter range 1 to 20 ..mu..m and relative refractive index 1.20 is measured. Particle size of narrowly dispersed populations is determined and a multi-modal dispersion of five components is completely analyzed. A 360/sup 0/ light scattering photometer for analysis of single particles has been designed and developed. A fluid stream containing single particles intersects a focused laser beam at the primary focal point of an ellip… more

Date: June 1, 1979

Creator: Bartholdi, M.F.

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

Flow cytometric applications of tumor biology: prospects and pitfalls. [Applications in study of spontaneous dog tumors and in drug and radiation effects on cultured V79 cells]

A brief review of cytometry instrumentation and its potential applications in tumor biology is presented using our recent data. Age-distribution measurements of cells from spontaneous dog tumors and cultured cells after exposure to x rays, alpha particles, or adriamycin are shown. The data show that DNA fluorescence measurements have application in the study of cell kinetics after either radiation or drug treatment. Extensive and careful experimentation is needed to utilize the sophisticated de… more

Date: January 1, 1979

Creator: Raju, M.R.; Johnson, T.S.; Tokita, N. & Gillette, E.L.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Flow cytometric analysis of respiratory tract cells exposed to oil shale and silica particulates. [Hamsters]

Flow cytometric techniques were used to measure the cytological and biochemical damage to respiratory tract cells in animals exposed to particulates. Hamsters were exposed to raw and spent oil shale particulates and silica by intratracheal instillation. Exfoliated lung cells were obtained by sacrificing the animals and lavaging the respiratory tract posterior to the trachea with saline. Cell samples were fixed in ethanol and stained with mithramycin for fluorescence analysis of DNA content. DNA… more

Date: January 1, 1979

Creator: Steinkamp, J. A. & Wilson, J. S.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Los Alamos Life Sciences Division's biomedical and environmental research programs. Progress report, January-December 1981. [Leading abstract]

This report summarizes research and development activities of the Los Alamos Life Sciences Division's Biomedical and Environmental Research program for the calendar year 1981. Individual reports describing the current status of projects have been entered individually into the data base.

Date: October 1, 1982

Creator: Holland, L.M. & Stafford, C.G. (comps.)

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

Heterogeneity in cytokinetic, clonogenic, and radiation response induced by nutrient deprivation

A murine fibrosarcoma cell line (FSA 1233), with a capability to grow in vitro, was used to study biophysical heterogeneity induced by nutrient deprivation. Cell sub-populations were isolated by centrifuging cells through linear Renografin-density gradients. The effects of cell growth to late plateau phase on the cytokinetic, clonogenic, and radiation-survival characteristics were studied. It was observed that with increasing age of the cell culture, an increase in the proportion of relatively … more

Date: January 1, 1986

Creator: Grdina, D.J. (Argonne National Lab., IL (USA)); Williamson, K.D.; Johnson, T.S. (Anderson (M.D.) Hospital and Tumor Inst., Houston, TX (USA)) & Sigdestad, C.P. (Louisville Univ., KY (USA). Dept. of Radiation Oncology)

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

Flow cytometry for health monitoring in space

Monitoring the health of space station or lunar base residents will be necessary to provide knowledge of the physiological status of astronauts. Flow cytometric techniques are uniquely capable of providing cellular, chromosome, hormone level and enzyme level information. The use of dyes provides the basis for fluorescently labeling specific cellular components. Laser induced fluorescence from stained cells is quantitated in a flow cytometer to measure cellular components such as DNA, RNA and pr… more

Date: January 1, 1984

Creator: Jett, J.H.; Martin, J.C.; Saunders, G.C. & Stewart, C.C.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Flow cytometric methods for assaying damage to respiratory tract cells. [Hamsters exposed to agents associated with production of synthetic fuels from oil shale]

This paper summarizes results of experiments designed to develop automated flow-analysis assay methods for discerning damage to exfoliated respiratory tract cells in model test animals exposed by inhalation to physical and chemical agents associated with the production of synthetic fuels from oil shale, the specific goal being the determination of atypical changes in exposed lung macrophages and epithelial cells. Animals were exposed to oil shale particulates (raw and spent), silica, and ozone,… more

Date: January 1, 1979

Creator: Steinkamp, J. A. & Wilson, J. S.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Modular software for flow cytometry and sorting: the LACEL system

We present an overview of the software for a flexible data acquisition system for multiple flow cytometers. Together with the hardware, which is discussed in a companion paper, the LACEL (Los Alamos Cell Analysis) system provides the capability to acquire data on up to 8 parameters and control bidirectional sorting based on up to 4 parameters for each direction. The two major software modules include a user interface which accepts commands and displays frequency distributions, and a data acquis… more

Date: January 1, 1979

Creator: Salzman, G.C.; Wilkins, S.F. & Whitfill, J.A.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Fluorescence polarization and pulse width analysis of chromosomes by a flow system. [Hamsters]

Isolated Chinese hamster chromosomes have been analyzed using a multiparameter computer-controlled cell sorter (MACCS) to obtain information about unique properties of individual chromosomes. Parameters other than DNA content were sought that would further aid in distinguishing among chromosomes. The polarized emission of Hoechst 33342 was measured for each class of chromosomes identified by a distinct peak. The emission anisotropy values for every chromosome class was a constant (EA = 0.30) wi… more

Date: January 1, 1978

Creator: Cram, L.S.; Ardnt-Jovin, D.J.; Grimwade, B.G. & Jovin, T.M.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Dual-laser flow cytometry of single mammalian cells. [Performance of low-power argon and high-power krypton laser system for quantitative analysis and sorting of cells]

An improved dual-laser flow cytometric system for quantitative analysis and sorting of mammalian cells has been developed using a low-power argon and high-power krypton laser as illumination sources, thus permitting the excitation of fluorescent dyes having absorption regions ranging from the ultraviolet to infrared. Cells stained in liquid suspension with fluorescent dyes enter a flow chamber where they intersect two spatially separated laser beams. Separate pairs of quartz beam-shaping optics… more

Date: April 23, 1978

Creator: Steinkamp, J.A.; Orlicky, D.J. & Crissman, H.A.

Item Type: Article

Partner: UNT Libraries Government Documents Department