Laser flow cytophotometric light scatter and fluorescence time-of-flight sizing of mammalian cells. [Use of pulse width or pulse rise time measurements]

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In laser flow cytophotometry, an increasingly popular technique of analytical cytology, quantitative measurements of interest include cell and nuclear diameters. Electronic circuitry for a new method of time-of-flight cell sizing was developed which measures the time that signal pulses from either fluorescence or light scatter sensors exceed a preset constant fraction of the peak signal amplitude (pulse width) or the time that it takes a signal to rise between constant fractions of the peak signal amplitude on the rising side of the pulse (pulse rise-time). These pulse width or pulse rise-time measurements were related to cell or nuclear diameters and ... continued below

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Pages: 26

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Leary, J.F.; Todd, P.; Wood, J.C.S. & Jett, J.H. January 1, 1978.

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Description

In laser flow cytophotometry, an increasingly popular technique of analytical cytology, quantitative measurements of interest include cell and nuclear diameters. Electronic circuitry for a new method of time-of-flight cell sizing was developed which measures the time that signal pulses from either fluorescence or light scatter sensors exceed a preset constant fraction of the peak signal amplitude (pulse width) or the time that it takes a signal to rise between constant fractions of the peak signal amplitude on the rising side of the pulse (pulse rise-time). These pulse width or pulse rise-time measurements were related to cell or nuclear diameters and were used in combination to determine nuclear size to cell size ratios. This new type of time-of-flight sizing was found to be independent of fluorescent or light-absorbing stain intensity, linearly related to cell or nuclear diameter, and capable of resolving diameter differences of less than 0.5 ..mu..m.

Physical Description

Pages: 26

Notes

Dep. NTIS, PC A03/MF A01.

Source

  • 6. conference on automated cytology, Elmau, F.R. Germany, 23 Apr 1978

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  • Report No.: LA-UR-78-1994
  • Report No.: CONF-780488-8
  • Grant Number: W-7405-ENG-36
  • Office of Scientific & Technical Information Report Number: 6205393
  • Archival Resource Key: ark:/67531/metadc1113473

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Office of Scientific & Technical Information Technical Reports

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Creation Date

  • January 1, 1978

Added to The UNT Digital Library

  • Feb. 22, 2018, 7:45 p.m.

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  • May 31, 2018, 12:21 p.m.

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Leary, J.F.; Todd, P.; Wood, J.C.S. & Jett, J.H. Laser flow cytophotometric light scatter and fluorescence time-of-flight sizing of mammalian cells. [Use of pulse width or pulse rise time measurements], article, January 1, 1978; New Mexico. (digital.library.unt.edu/ark:/67531/metadc1113473/: accessed September 20, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.