Three-dimensional lithographically-defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression
Description:
Here we describe a simple micromolding method to construct three-dimensional arrays of organotypic epithelial tissue structures that approximate in vivo histology. An elastomeric stamp containing an array of posts of defined geometry and spacing is used to mold microscale cavities into the surface of type I collagen gels. Epithelial cells are seeded into the cavities and covered with a second layer of collagen. The cells reorganize into hollow tissues corresponding to the geometry of the caviti…
more
Date:
February 13, 2008
Creator:
Nelson, Celeste M.; Inman, Jamie L. & Bissell, Mina J.
Item Type:
Refine your search to only
Article
Partner:
UNT Libraries Government Documents Department