Three-dimensional lithographically-defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression

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Here we describe a simple micromolding method to construct three-dimensional arrays of organotypic epithelial tissue structures that approximate in vivo histology. An elastomeric stamp containing an array of posts of defined geometry and spacing is used to mold microscale cavities into the surface of type I collagen gels. Epithelial cells are seeded into the cavities and covered with a second layer of collagen. The cells reorganize into hollow tissues corresponding to the geometry of the cavities. Patterned tissue arrays can be produced in 3-4 h and will undergo morphogenesis over the following one to three days. The protocol can easily ... continued below

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Nelson, Celeste M.; Inman, Jamie L. & Bissell, Mina J. February 13, 2008.

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Description

Here we describe a simple micromolding method to construct three-dimensional arrays of organotypic epithelial tissue structures that approximate in vivo histology. An elastomeric stamp containing an array of posts of defined geometry and spacing is used to mold microscale cavities into the surface of type I collagen gels. Epithelial cells are seeded into the cavities and covered with a second layer of collagen. The cells reorganize into hollow tissues corresponding to the geometry of the cavities. Patterned tissue arrays can be produced in 3-4 h and will undergo morphogenesis over the following one to three days. The protocol can easily be adapted to study a variety of tissues and aspects of normal and neoplastic development.

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  • Journal Name: Nature Protocols; Journal Volume: 3; Related Information: Journal Publication Date: 2008

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  • Report No.: LBNL-36E
  • Grant Number: DE-AC02-05CH11231
  • DOI: 10.1038/nprot.2008.35 | External Link
  • Office of Scientific & Technical Information Report Number: 937441
  • Archival Resource Key: ark:/67531/metadc900915

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  • February 13, 2008

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  • Sept. 27, 2016, 1:39 a.m.

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  • Sept. 30, 2016, 7:17 p.m.

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Nelson, Celeste M.; Inman, Jamie L. & Bissell, Mina J. Three-dimensional lithographically-defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression, article, February 13, 2008; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc900915/: accessed August 17, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.