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Genetic changes in Mammalian cells transformed by helium cells

Description: Midterm Syrian Hamster embryo (SHE) cells were employed to study high LET-radiation induced tumorigenesis. Normal SHE cells (secondary passage) were irradiated with accelerated helium ions at an incident energy of 22 MeV/u (9--10 keV/{mu}m). Transformed clones were isolated after growth in soft agar of cells obtained from the foci of the initial monolayer plated postirradiation. To study the progression process of malignant transformation, the transformed clones were followed by monolayer subcu… more
Date: November 1, 1990
Creator: Durante, M.; Grossi, G. (Naples Univ. (Italy). Dipt. di Scienze Fisiche); Yang, T.C. & Roots, R. (Lawrence Berkeley Lab., CA (USA))
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Partner: UNT Libraries Government Documents Department
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Chromosomal changes in cultured human epithelial cells transformed by low- and high-LET radiation

Description: For a better assessment of radiation risk in space, an understanding of the responses of human cells, especially the epithelial cells, to low- and high-LET radiation is essential. In our laboratory, we have successfully developed techniques to study the neoplastic transformation of two human epithelial cell systems by ionizing radiation. These cell systems are human mammary epithelial cells (H184B5) and human epidermal keratinocytes (HEK). Both cell lines are immortal, anchorage dependent for g… more
Date: November 1, 1990
Creator: Yang, Tracy Chui-hsu; Craise, L. M.; Prioleau, J. C.; Stampfer, M. R. & Rhim, J. S.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Ultrafast nanolaser device for detecting cancer in a single live cell.

Description: Emerging BioMicroNanotechnologies have the potential to provide accurate, realtime, high throughput screening of live tumor cells without invasive chemical reagents when coupled with ultrafast laser methods. These optically based methods are critical to advancing early detection, diagnosis, and treatment of disease. The first year goals of this project are to develop a laser-based imaging system integrated with an in- vitro, live-cell, micro-culture to study mammalian cells under controlled con… more
Date: November 1, 2007
Creator: Gourley, Paul Lee & McDonald, Anthony Eugene
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Segmentation of Three Dimensional Cell Culture Models from aSingle Focal Plane

Description: Three dimensional cell culture models offer new opportunities for development of computational techniques for segmentation and localization. These assays have a unique signature of a clump of cells that correspond to a functioning colony. Often the nuclear compartment is labeled and then imaged with fluorescent microscopy to provide context for protein localization. These colonies are first delineated from background using the level set method. Within each colony, nuclear regions are then bound… more
Date: November 1, 2006
Creator: Chang, Hang & Parvin, Bahram
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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UV-induced changes in cell cycle and gene expression within rabbit lens epithelial cells

Description: Damage to lens epithelial cells is a probable initiation process in cataract formation induced by ultraviolet radiation. These experiments investigated the ability of 254 nm radiation on cell cycle progression and gene expression in rabbit lens epithelial cell line N/N1003A. No changes in expression of c-fos, c-jun, alpha- tubulin, or vimentin was observed following UV exposure. Using flow cytometry, an accumulation of cells in G1/S phase of the cell cycle 1 hr following exposure. The observed … more
Date: November 1, 1994
Creator: Sidjanin, Duska; Grdina, David & Woloschak, Gayle E.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Bench-scale demonstration of biological production of ethanol from coal synthesis gas. Topical report 5, Process analysis

Description: The economics of converting coal to ethanol by a biological process is quite attractive. When processing 1500 tons of coal per day, the plant generates 85 million gallons of ethanol per year. The return on investment for the process is 110 percent and the payout is 0.9 years.
Date: November 1, 1995
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Processing of DNA damage after exposure to a single dose of fission spectrum neutrons takes 40 hours to complete

Description: We have examined the time course over a period of days of repair of chromosomal single-strand breaks (SSB) induced by a single dose of JANUS fission-spectrum neutrons in the DNA of human P3 epithelial teratocarcinoma cells. When the cells are allowed a period of repair incubation the breaks are totally sealed by 7 hours. But then following these initial repair the DNA is dismantled as evidenced by the reappearance of SSBs. This secondary breakage is almost as extensive as that caused by the ori… more
Date: November 1, 1996
Creator: Peak, J.G. & Peak, M.J.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Spectroscopic Detection of Pathogens

Description: The goal of this LDRD Research project was to provide a preliminary examination of the use of infrared spectroscopy as a tool to detect the changes in cell cultures upon activation by an infectious agent. Due to a late arrival of funding, only 5 months were available to transfer and setup equipment at UTTM,develop cell culture lines, test methods of in-situ activation and collect kinetic data from activated cells. Using attenuated total reflectance (ATR) as a sampling method, live cell cultures… more
Date: November 1, 2000
Creator: ALAM,M. KATHLEEN; TIMLIN,JERILYN A.; MARTIN,LAURA E.; HJELLE,DRIAN; LYONS,RICK & GARRISON,KRISTIN
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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