Processing of DNA damage after exposure to a single dose of fission spectrum neutrons takes 40 hours to complete

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We have examined the time course over a period of days of repair of chromosomal single-strand breaks (SSB) induced by a single dose of JANUS fission-spectrum neutrons in the DNA of human P3 epithelial teratocarcinoma cells. When the cells are allowed a period of repair incubation the breaks are totally sealed by 7 hours. But then following these initial repair the DNA is dismantled as evidenced by the reappearance of SSBs. This secondary breakage is almost as extensive as that caused by the original neutron exposure, with a maximum at 16-18 hours. Finally, the DNA is rejoined, regaining its original ... continued below

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22 p.

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Peak, J.G. & Peak, M.J. November 1, 1996.

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Description

We have examined the time course over a period of days of repair of chromosomal single-strand breaks (SSB) induced by a single dose of JANUS fission-spectrum neutrons in the DNA of human P3 epithelial teratocarcinoma cells. When the cells are allowed a period of repair incubation the breaks are totally sealed by 7 hours. But then following these initial repair the DNA is dismantled as evidenced by the reappearance of SSBs. This secondary breakage is almost as extensive as that caused by the original neutron exposure, with a maximum at 16-18 hours. Finally, the DNA is rejoined, regaining its original size by 40 hours after irradiation. The secondary repair phenomenon may have an editing function, or it many represent the processing of residual damage left unrepaired during the initial rejoining of the backbone breaks.

Physical Description

22 p.

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OSTI as DE97000558

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  • Other Information: PBD: [1996]

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  • Other: DE97000558
  • Report No.: ANL/BIM/PP--72359
  • Grant Number: W-31109-ENG-38
  • DOI: 10.2172/414030 | External Link
  • Office of Scientific & Technical Information Report Number: 414030
  • Archival Resource Key: ark:/67531/metadc674516

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Office of Scientific & Technical Information Technical Reports

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  • November 1, 1996

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  • July 25, 2015, 2:20 a.m.

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  • June 10, 2016, 9:17 p.m.

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Peak, J.G. & Peak, M.J. Processing of DNA damage after exposure to a single dose of fission spectrum neutrons takes 40 hours to complete, report, November 1, 1996; Illinois. (digital.library.unt.edu/ark:/67531/metadc674516/: accessed December 14, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.