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Pacific Northwest Laboratory annual report for 1985 to the DOE Office of Energy Research. Part 4. Physical sciences

Description: Part 4 of the Pacific Northwest Laboratory Annual Report for 1985 to the DOE Office of Energy Research includes those programs funded under the title ''Physical and Technological Research.'' The Field Task Program Studies reports in this document are grouped by budget category and each section is introduced by an abstract that indicates the Field Task Proposal/Agreement reported in that section. These reports only briefly indicate progress made during 1985. The reader should contact the princip… more
Date: February 1, 1986
Creator: Toburen, L. H.
Partner: UNT Libraries Government Documents Department
open access

Livermore flow cytometer

Description: A stable, easy to operate, flow cytometer was built for routine use by research personnel in a biology laboratory. A 5 watt argon-ion laser excites the fluorescent dyes in cells flowing through a quartz flow chamber. A f/0.95 TV camera lens collects the fluorescent and scattered light and directs it through color separation filters and a beam splitter onto two photomultiplier tubes. Two 3-axis positioners are used to align the focused laser beam and the flow chamber with the light collection op… more
Date: November 19, 1981
Creator: Peters, D.C.; Dean, P.N. & Pinkel, D.
Partner: UNT Libraries Government Documents Department
open access

Single particle analysis with a 360/sup 0/ light scattering photometer

Description: Light scattering by single spherical homogeneous particles in the diameter range 1 to 20 ..mu..m and relative refractive index 1.20 is measured. Particle size of narrowly dispersed populations is determined and a multi-modal dispersion of five components is completely analyzed. A 360/sup 0/ light scattering photometer for analysis of single particles has been designed and developed. A fluid stream containing single particles intersects a focused laser beam at the primary focal point of an ellip… more
Date: June 1, 1979
Creator: Bartholdi, M.F.
Partner: UNT Libraries Government Documents Department
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Los Alamos Life Sciences Division's biomedical and environmental research programs. Progress report, January-December 1981. [Leading abstract]

Description: This report summarizes research and development activities of the Los Alamos Life Sciences Division's Biomedical and Environmental Research program for the calendar year 1981. Individual reports describing the current status of projects have been entered individually into the data base.
Date: October 1, 1982
Creator: Holland, L.M. & Stafford, C.G. (comps.)
Partner: UNT Libraries Government Documents Department
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Heterogeneity in cytokinetic, clonogenic, and radiation response induced by nutrient deprivation

Description: A murine fibrosarcoma cell line (FSA 1233), with a capability to grow in vitro, was used to study biophysical heterogeneity induced by nutrient deprivation. Cell sub-populations were isolated by centrifuging cells through linear Renografin-density gradients. The effects of cell growth to late plateau phase on the cytokinetic, clonogenic, and radiation-survival characteristics were studied. It was observed that with increasing age of the cell culture, an increase in the proportion of relatively … more
Date: January 1, 1986
Creator: Grdina, D.J. (Argonne National Lab., IL (USA)); Williamson, K.D.; Johnson, T.S. (Anderson (M.D.) Hospital and Tumor Inst., Houston, TX (USA)) & Sigdestad, C.P. (Louisville Univ., KY (USA). Dept. of Radiation Oncology)
Partner: UNT Libraries Government Documents Department
open access

Flow cytometric quantification of radiation responses of murine peritoneal cells

Description: Methods have been developed to distinguish subpopulations of murine peritoneal cells, and these were applied to the measurement of early changes in peritoneal cells after irradiation. The ratio of the two major subpopulations in the peritoneal fluid, lymphocytes and macrophages, was measured rapidly by means of cell volume distribution analysis as well as by hypotonic propidium iodide (PI) staining. After irradiation, dose and time dependent changes were noted in the cell volume distributions: … more
Date: January 1, 1982
Creator: Tokita, N. & Raju, M.R.
Partner: UNT Libraries Government Documents Department
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Effect of elevated temperatures on cell cycle kinetics of rat gliosarcoma cells

Description: 9L rat gliosarcoma cells were examined in vitro for survival response to hyperthermic temperatures ranging from 39.0/sup 0/ to 45.0/sup 0/C for graded exposure times. At 43.0/sup 0/C, the split exposure response was also studied. Changes in cell cycle kinetics resulting from hyperthermia were compared for isosurvival levels achieved by appropriate exposure time to either 42.5/sup 0/C or 43.0/sup 0/C. After heat treatment, cells were held at 37.0/sup 0/C for varying recovery periods. Cells were … more
Date: July 1, 1978
Creator: Ross-Riveros, P.
Partner: UNT Libraries Government Documents Department
open access

Autonomous Pathogen Detection System FY02 Annual Progress Report

Description: The objective of this project is to design, fabricate and field demonstrate a biological agent detection and identification capability, the Autonomous Pathogen Detector System (APDS). Integrating a flow cytometer and real-time polymerase chain reaction (PCR) detector with sample collection, sample preparation and fluidics will provide a compact, autonomously operating instrument capable of simultaneously detecting multiple pathogens and/or toxins. The APDS will operate in fixed locations, conti… more
Date: November 11, 2002
Creator: Colston, B.; Brown, S.; Burris, K.; Elkin, C.; Hindson, B.; Langlois, R. et al.
Partner: UNT Libraries Government Documents Department
open access

Kinetic and biochemical studies on tumor growth. Final report, October 1, 1967--July 14, 1976. [X radiation, /sup 125/I tracer technique]

Description: The normal growth kinetics and the recovery kinetics following therapeutic perturbations with drugs and x-irradiation for the mouse duodenal crypt cells and the S102F and slow line tumor cells have been studied in depth, the rationale being that a detailed understanding of these phenomena would enhance our knowledge about tumor biology per se and also expand our appreciation of how normal and malignant tissues respond to and recover from therapeutic insults. Hopefully, such knowledge will event… more
Date: unknown
Creator: Dethlefsen, L. A.
Partner: UNT Libraries Government Documents Department
open access

(Controls of the plant endomembrane-secretory pathway)

Description: These studies are focused on elucidating the molecular structure of plant cell membranes with special reference to cell surface glycoproteins. The studies reported herein include use of monoclonal antibodies to characterize cell surface epitopes, construction of cDNA libraries of cell surface proteins, isolation of plant cell mutants by flow cytometry, detection of beta-glucouronidase marker enzyme systems in plants, expression go VSVG (the major envelope glycoprotein of Vesicular Stomatis Viru… more
Date: January 1, 1991
Partner: UNT Libraries Government Documents Department
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[Vertically intergrated analysis of human DNA]. Progress report, [April 1, 1993--February 28, 1994]

Description: Progress is reported on several fronts aimed at DNA sequencing using cosmids-contigs. Specifically results gained in exploiting RARE (recA-assisted-restriction enzyme) cleavage, in clone isolation by flow cytometry, in cosmid-DNA preparations, in automated interpretation of digitized gel images and in contig building via multiple complete digest (MCD) mapping are briefly described.
Date: March 1, 1994
Creator: Olson, M. V.
Partner: UNT Libraries Government Documents Department
open access

[Controls of the plant endomembrane-secretory pathway]. Final report

Description: These studies are focused on elucidating the molecular structure of plant cell membranes with special reference to cell surface glycoproteins. The studies reported herein include use of monoclonal antibodies to characterize cell surface epitopes, construction of cDNA libraries of cell surface proteins, isolation of plant cell mutants by flow cytometry, detection of beta-glucouronidase marker enzyme systems in plants, expression go VSVG (the major envelope glycoprotein of Vesicular Stomatis Viru… more
Date: December 31, 1991
Partner: UNT Libraries Government Documents Department
open access

Biopsy examination during the course of radiotherapy. Progress report. [DNA content of tumor cells in biopsy samples determined by microfluorometric or multiangle light-scatter spectrometry]

Description: Studies begun during the first year of this project on biopsies from spontaneous tumors in dogs and from human patients undergoing radiotherapy treatment were continued. The results indicate that significant fractions of the spontaneous tumors in dogs and cervical carcinomas in patients undergoing radiotherapy have a DNA content higher than normal diploid cells. The higher DNA content in tumor cells permits distinguishing them from normal cells in the biopsy material with the aid of flow microf… more
Date: December 1, 1977
Creator: Raju, M. R.
Partner: UNT Libraries Government Documents Department
open access

Ultrastructural and flow cytometric analyses of lipid accumulation in microalgae

Description: Lipid accumulation in three species of microalgae was investigated with flow cytometry (FCM) and transmission electron microscopy (TEM). Previous studies using batch cultures of a algae have led to the assumption that lipid accumulation in microalgae is a gradual process requiring at least several days for completion. However, FCM reveals, through changes in the chlorophyll:lipid ratio, that the time span required for individual cells to change metabolic state is short. Simultaneous FCM measure… more
Date: December 1, 1986
Creator: Solomon, J. A.; Hand, R. E., Jr. & Mann, R. C.
Partner: UNT Libraries Government Documents Department
open access

Autonomous pathogen detection system 2001

Description: The objective of this project is to design, fabricate and field-demonstrate a fully Autonomous Pathogen Detector (identifier) System (APDS). This will be accomplished by integrating a proven flow cytometer and real-time polymerase chain reaction (PCR) detector with sample collection, sample preparation and fluidics to provide a compact, autonomously operating instrument capable of simultaneously detecting multiple pathogens and/or toxins. The APDS will be designed to operate in fixed locations,… more
Date: January 9, 2001
Creator: Langlois, R. G.; Wang, A.; Colston, B.; Masquelier, D.; Jones, L.; Venkateswaran, K. S. et al.
Partner: UNT Libraries Government Documents Department
open access

Final Report Nucleic Acid System - Hybrid PCR and Multiplex Assay Project Phase 2

Description: This report covers phase 2 (year 2) of the Nucleic Acid System--Hybrid PCR and Multiplex Assay project. The objective of the project is to reduce to practice the detection and identification of biological warfare pathogens by the nucleic acid recognition technique of PCR (polymerase chain reaction) in a multiplex mode using flow cytometry. The Hybrid instrument consists of a flow-through PCR module capable of handling a multiplexed PCR assay, a hybridizing module capable of hybridizing multiple… more
Date: April 17, 2002
Creator: Koopman, R. P.; Langlois, R. G.; Nasarabadi, S.; Benett, W. J.; Colston, B. W.; Johnson, D. C. et al.
Partner: UNT Libraries Government Documents Department
open access

Life Sciences Division annual report, 1988

Description: This report summarizes the research and development activities of Los Alamos National Laboratory's Life Sciences Division for the calendar year 1988. Technical reports related to the current status of projects are presented in sufficient detail to permit the informed reader to assess their scope and significance. Summaries useful to the casual reader desiring general information have been prepared by the Group Leaders and appear in each group overview. Investigators on the staff of the Life Sci… more
Date: April 1, 1989
Creator: Marrone, B. L. & Cram, L. S. (comps.)
Partner: UNT Libraries Government Documents Department
open access

DNA sequencing with capillary electrophoresis and single cell analysis with mass spectrometry

Description: Since the first demonstration of the laser in the 1960`s, lasers have found numerous applications in analytical chemistry. In this work, two different applications are described, namely, DNA sequencing with capillary gel electrophoresis and single cell analysis with mass spectrometry. Two projects are described in which high-speed DNA separations with capillary gel electrophoresis were demonstrated. In the third project, flow cytometry and mass spectrometry were coupled via a laser vaporization… more
Date: March 27, 1998
Creator: Fung, N.
Partner: UNT Libraries Government Documents Department
open access

Measurement of Synechococcus in situ growth rates using flow cytometry and rRNA-targeted probes. Final report

Description: The overall goal of this project was the development of methods for the estimation of in situ Synechococcus growth rates using flow cytometrically-measured cellular properties. As an important picoplanktonic primary producer, Synechococcus can be expected to significantly influence the cycling of carbon in coastal marine environments. Traditional methods for estimating growth rates in natural populations of these and other phytoplankton have relied upon bottle incubations, which take a long tim… more
Date: February 18, 1998
Creator: Chisholm, S. W. & Binder, B. J.
Partner: UNT Libraries Government Documents Department
open access

National flow cytometry and sorting research resource. Annual progress report, July, 1, 1994--June 30, 1995, Year 12

Description: Research progress utilizing flow cytometry is described. Topics include: rapid kinetics flow cytometry; characterization of size determinations for small DNA fragments; statistical analysis; energy transfer measurements of molecular confirmation in micelles; and enrichment of Mus spretus chromosomes by dual parameter flow sorting and identification of sorted fractions by fluorescence in-situ hybridization onto G-banded mouse metaphase spreads.
Date: April 27, 1995
Creator: Jett, J. H.
Partner: UNT Libraries Government Documents Department
open access

Flow karyotyping and flow instrumentation development. Final report

Description: The project had three major aims: improvement of technology for high-speed cell and chromosome sorting; the use of such instrumentation in genome analysis; applying the principles developed and the lessons learned to automated processes for the genome program. The work was a continuation of studies that were started at the Lawrence Livermore National Laboratory before the PI moved to the University of Washington. At Livermore, a high-speed sorter for the selection of human chromosomes was built… more
Date: November 1, 1997
Creator: GEngh, G.J. van den
Partner: UNT Libraries Government Documents Department
open access

Sample handling for kinetics and molecular assembly in flow cytometry

Description: Flow cytometry discriminates particle associated fluorescence from the fluorescence of the surrounding medium. It permits assemblies of macromolecular complexes on beads or cells to be detected in real-time with precision and specificity. The authors have investigated two types of robust sample handling systems which provide sub-second resolution and high throughput: (1) mixers which use stepper-motor driven syringes to initiate chemical reactions in msec time frames; and (2) flow injection con… more
Date: July 1998
Creator: Sklar, L. A.; Seamer, L. C.; Kuckuck, F.; Prossnitz, E.; Edwards, B. & Posner, G.
Partner: UNT Libraries Government Documents Department
open access

Flow cytometry-based DNA hybridization and polymorphism analysis

Description: Functional analysis of the humane genome, including the quantification of differential gene expression and the identification of polymorphic sites and disease genes, is an important element of the Human Genome Project. Current methods of analysis are mainly gel-based assays that are not well-suited to rapid genome-scale analyses. To analyze DNA sequence on a large scale, robust and high throughput assays are needed. The authors are developing a suite of microsphere-based approaches employing fl… more
Date: July 1, 1998
Creator: Cai, Hong; Kommander, K.; White, P. S. & Nolan, J. P.
Partner: UNT Libraries Government Documents Department
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