DNA sequencing with capillary electrophoresis and single cell analysis with mass spectrometry

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Description

Since the first demonstration of the laser in the 1960`s, lasers have found numerous applications in analytical chemistry. In this work, two different applications are described, namely, DNA sequencing with capillary gel electrophoresis and single cell analysis with mass spectrometry. Two projects are described in which high-speed DNA separations with capillary gel electrophoresis were demonstrated. In the third project, flow cytometry and mass spectrometry were coupled via a laser vaporization/ionization interface and individual mammalian cells were analyzed. First, DNA Sanger fragments were separated by capillary gel electrophoresis. A separation speed of 20 basepairs per minute was demonstrated with a mixed ... continued below

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89 p.

Creation Information

Fung, N. March 27, 1998.

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This report is part of the collection entitled: Office of Scientific & Technical Information Technical Reports and was provided by UNT Libraries Government Documents Department to Digital Library, a digital repository hosted by the UNT Libraries. More information about this report can be viewed below.

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  • Ames Laboratory
    Publisher Info: Ames Lab., IA (United States)
    Place of Publication: Iowa

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Description

Since the first demonstration of the laser in the 1960`s, lasers have found numerous applications in analytical chemistry. In this work, two different applications are described, namely, DNA sequencing with capillary gel electrophoresis and single cell analysis with mass spectrometry. Two projects are described in which high-speed DNA separations with capillary gel electrophoresis were demonstrated. In the third project, flow cytometry and mass spectrometry were coupled via a laser vaporization/ionization interface and individual mammalian cells were analyzed. First, DNA Sanger fragments were separated by capillary gel electrophoresis. A separation speed of 20 basepairs per minute was demonstrated with a mixed poly(ethylene oxide) (PEO) sieving solution. In addition, a new capillary wall treatment protocol was developed in which bare (or uncoated) capillaries can be used in DNA sequencing. Second, a temperature programming scheme was used to separate DNA Sanger fragments. Third, flow cytometry and mass spectrometry were coupled with a laser vaporization/ionization interface.

Physical Description

89 p.

Notes

OSTI as DE99002506

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  • Other Information: DN: Thesis submitted to Iowa State Univ., Ames, IA (US); TH: Thesis (Ph.D.)

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  • Other: DE99002506
  • Report No.: IS-T--1841
  • Grant Number: W-7405-ENG-82
  • DOI: 10.2172/348902 | External Link
  • Office of Scientific & Technical Information Report Number: 348902
  • Archival Resource Key: ark:/67531/metadc682891

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  • March 27, 1998

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  • July 25, 2015, 2:20 a.m.

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  • Nov. 6, 2015, 8:55 p.m.

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Fung, N. DNA sequencing with capillary electrophoresis and single cell analysis with mass spectrometry, report, March 27, 1998; Iowa. (digital.library.unt.edu/ark:/67531/metadc682891/: accessed September 25, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.