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Phase-sensitive flow cytometer

Description: This report describes phase-sensitive flow cytometer (FCM) which provides additional FCM capability to use the fluorescence lifetime of one or more fluorochromes bound to single cells to provide additional information regarding the cells. The resulting fluorescence emission can be resolved into individual fluorescence signals if two fluorochromes are present or can be converted directly to a decay lifetime from a single fluorochrome. The excitation light for the fluorochromes is modulated to pr… more
Date: December 31, 1992
Creator: Steinkamp, J. A.
Partner: UNT Libraries Government Documents Department
open access

DNA fragment sizing and sorting by laser-induced fluorescence

Description: A method is provided for obtaining DNA fingerprints using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a selected sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to … more
Date: December 31, 1992
Creator: Jett, J. H.; Hammond, M. L.; Keller, R. A.; Marrone, B. L. & Martin, J. C.
Partner: UNT Libraries Government Documents Department
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