Chemical Mechanism of the Catalytic Subunit of Camp-Dependent Protein Kinase: Methods for Determining the Primary ¹⁸O Isotope Effects Using the Remote Label Technique
Description:
A description of the nature of the transition state structure for phosphoryl transfer in the cAPK reaction requires a measurement of the primary 180 isotope effect at the serine hydroxyl acceptor. Since it is difficult to obtain primary 180 isotope effect directly, the 15N/1 4N ratio of the a-amine of the C-terminal glycine in the peptide Leu Arg-Lys-Ala-Ser-Leu-Gly (when serine is phosphorylated) was used to represent on the phosphorylation at serine. 15N Glycine, ' 4N-Glycine and 180 serine w…
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Date:
December 1991
Creator:
Chen, Gang, 1963-
Partner:
UNT Libraries