Creation and Characterization of an Escherichia Coli and Pseudomonas Putida Hybrid Aspartate Transcarbamoylase

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Aspartate transcarbamoylase (ATCase) is encoded by the pyrBI genes in E. coli. Expression of these genes is reduced four-fold by attenuation when grown on uracil. Using plasmid, pRO1727. the pyrB and the pyrBI genes from E. coli were cloned into a P. putida pyrB auxotroph. A recombinant pyrB gene was recovered that encoded a functional hybrid ATCase with a molecular weight of 470 kDa.

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Ruley, Jill R. (Jill Rosanne) December 1992.

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Aspartate transcarbamoylase (ATCase) is encoded by the pyrBI genes in E. coli. Expression of these genes is reduced four-fold by attenuation when grown on uracil. Using plasmid, pRO1727. the pyrB and the pyrBI genes from E. coli were cloned into a P. putida pyrB auxotroph. A recombinant pyrB gene was recovered that encoded a functional hybrid ATCase with a molecular weight of 470 kDa.

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  • December 1992

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  • Jan. 14, 2016, 8:34 p.m.

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  • April 21, 2023, 4:13 p.m.

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Ruley, Jill R. (Jill Rosanne). Creation and Characterization of an Escherichia Coli and Pseudomonas Putida Hybrid Aspartate Transcarbamoylase, thesis or dissertation, December 1992; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc798137/: accessed July 17, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .

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