Quantitative characterization of the protein contents of the exocrine pancreatic acinar cell by soft x-ray microscopy and advanced digital imaging methods Metadata

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Title

  • Main Title Quantitative characterization of the protein contents of the exocrine pancreatic acinar cell by soft x-ray microscopy and advanced digital imaging methods

Creator

  • Author: Loo, Billy W., Jr.
    Creator Type: Personal

Contributor

  • Sponsor: United States. Department of Energy. Office of Basic Energy Sciences.
    Contributor Type: Organization
    Contributor Info: USDOE Director, Office of Science. Office of Basic Energy Studies (United States)

Publisher

  • Name: Lawrence Berkeley National Laboratory
    Place of Publication: Berkeley, California
    Additional Info: Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (United States)

Date

  • Creation: 2000-06-09

Language

  • English

Description

  • Content Description: The study of the exocrine pancreatic acinar cell has been central to the development of models of many cellular processes, especially of protein transport and secretion. Traditional methods used to examine this system have provided a wealth of qualitative information from which mechanistic models have been inferred. However they have lacked the ability to make quantitative measurements, particularly of the distribution of protein in the cell, information critical for grounding of models in terms of magnitude and relative significance. This dissertation describes the development and application of new tools that were used to measure the protein content of the major intracellular compartments in the acinar cell, particularly the zymogen granule. Soft x-ray microscopy permits image formation with high resolution and contrast determined by the underlying protein content of tissue rather than staining avidity. A sample preparation method compatible with x-ray microscopy was developed and its properties evaluated. Automatic computerized methods were developed to acquire, calibrate, and analyze large volumes of x-ray microscopic images of exocrine pancreatic tissue sections. Statistics were compiled on the protein density of several organelles, and on the protein density, size, and spatial distribution of tens of thousands of zymogen granules. The results of these measurements, and how they compare to predictions of different models of protein transport, are discussed.
  • Physical Description: Medium: P; Size: 250 pages

Subject

  • Keyword: Sample Preparation
  • Keyword: Transport
  • STI Subject Categories: 59 Basic Biological Sciences
  • Keyword: Proteins
  • Keyword: Compartments
  • Keyword: Microscopy
  • Keyword: Spatial Distribution
  • Keyword: Cell Constituents
  • Keyword: Secretion
  • Keyword: Statistics
  • Keyword: Distribution
  • Keyword: Resolution

Source

  • Other Information: TH: Thesis (Ph.D.); Submitted to University of California, San Francisco, CA (US); Univ. of California, Berkeley, CA (US)

Collection

  • Name: Office of Scientific & Technical Information Technical Reports
    Code: OSTI

Institution

  • Name: UNT Libraries Government Documents Department
    Code: UNTGD

Resource Type

  • Thesis or Dissertation

Format

  • Text

Identifier

  • Report No.: LBNL--46128
  • Grant Number: AC03-76SF00098
  • Office of Scientific & Technical Information Report Number: 764399
  • Archival Resource Key: ark:/67531/metadc718708

Note

  • Display Note: OSTI as DE00764399
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