Radiation Effects Research Foundation five year strategic research plan and program management, 1997-2001 Page: 65 of 152
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!651 Using conventional staining methods, an extensive cytogenetic survey was conducted in
$52 the past involving nearly 16,000 F, persons (8,000 born to exposed parent(s) and 8,000 to the
53 unexposed). The results showed only one de novo autosomal mutation in each group, although
104 not all of the parents of aberration-carrying individuals could be cytogenetically examined. Thus,
655 no evidence of a radiation effect on the germ cells has been observed so far.
56
07 Major research activities in the next five years
658
659 Priority 1
1 C-1. Examination of EBV-transformed B-cell lines by the G-banding method
2
3 Because the previously used conventional staining method can detect only gross structural
changes and is not suitable for detecting small deletions, it will be necessary to apply the
5 G-banding method which can detect small changes.
6
7 EBV-transformed B-cell lines established from trios of families for molecular genetic
8 studies in the Laboratory of Biochemical Genetics would be an appropriate source of
9 materials, both in terms of sample size, estimated gonadal dose, and availability of cells
70 from the parents. The work will require 3 to 4 years.
2 Priority 2
73
4 C-2. Development of FISH using a single probe
75
6 One application of the FISH technique is mapping using a single gene probe or a probe
for a specific DNA segment. In the future, in the Laboratory of Biochemical Genetics, we
8 anticipate that mutant genes will be detected using molecular analysis of the children born
19 to the survivors. FISH mapping of the mutated gene would be useful in characterizing the
0 mutation.
1
82 Project time lines (Cytogenetics)84
85
86
7
8
9Fiscal Year 1997 1998 1999 2000 2001
1. FISH examination of survivors =>
2. FISH by a single probe
3. Chromosome domain structure = =
4. Genetic instability = =
5. Assessment of DS86 errors
Biochemical Genetics Program
The primary objective of the biochemical genetics program is to determine whether an
increase in mutations measurable at the molecular level has occurred in the children born to A-
bomb exposed parents in Hiroshima and Nagasaki.58
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Radiation Effects Research Foundation five year strategic research plan and program management, 1997-2001, report, October 31, 1997; Washington D.C.. (https://digital.library.unt.edu/ark:/67531/metadc716813/m1/65/?rotate=90: accessed July 16, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.