Engineered Microbial Consortium for the Efficient Conversion of Biomass to Biofuels Page: 79
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until further use (maximum time < 20 minutes). Clostridium cellulolyticum was grown in
Clostridial medium containing 7.5 g L-1 of cellulose as the carbon source to an OD of 0.4-0.6. The
cultures were then used to inoculate Clostridial medium containing cellobiose and microcrystalline
cellulose as carbon sources respectively.
The set-up for this experiment was similar to the previously described set-ups but now with
the inclusion of the recombinant ZM4 pAA1 to test the activity of the plasmid-encoded genes in
the breakdown of cellobiose and to check whether this clone does in anyway enhance the
production of ethanol in the co-culture of ZM4 pAAl with Clostridium. For the mono cultures of
ZM4 pAAl, ZM4 WT and C. cellulolyticum, the medium was inoculated with 5% inoculum size
(v v-1) and for the co-culture medium was inoculated with 2.5% each of the inoculum size. For the
Clostridial medium containing cellobiose, 2 g L-1 of the oligosaccharide was used whereas for the
regular Clostridial medium, 7.5 g L- of cellulose was used.
0.10
SD4pAAl
0.08 - Mir.4r
0.06 -
0.04
0.00
Batch 1 Batch 2 Batch 3
5 Days
Figure 5.14: Ethanol production from cellobiose. Error bars represent standard deviations among
three replicates.79
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Anieto, Ugochukwu Obiakornobi. Engineered Microbial Consortium for the Efficient Conversion of Biomass to Biofuels, dissertation, August 2014; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc699973/m1/90/?rotate=180: accessed July 17, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .