Molecular Identification of a Functional Homologue of the Mammalian Fatty Acid Amide Hydrolase in Arabidopsis thaliana

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Article on the molecular identification of a functional homologue of the mammalian fatty acid amide hydrolase in Arabidopsis thaliana.

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8 p.

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Shrestha, Rhidaya; Dixon, R. A. & Chapman, Kent D. June 24, 2003.

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Article on the molecular identification of a functional homologue of the mammalian fatty acid amide hydrolase in Arabidopsis thaliana.

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8 p.

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Abstract: N-Acylethanolamines (NAEs) are endogenous constituents of plant and animal tissues, and in vertebrates their hydrolysis terminates their participation as lipid mediators in the endocannabinoid signaling system. The membrane-bound enzyme responsible for NAE hydrolysis in mammals has been identified at the molecular level (designated fatty acid amide hydrolase, FAAH), and although an analogous enzyme activity was identified in microsomes of cotton seedlings, no molecular information is available for this enzyme in plants. Here we report the identification, the heterologous expression (in Escherichia coli), and the biochemical characterization of an Arabidopsis thaliana FAAH homologue. Candidate Arabidopsis DNA sequences containing a characteristic amidase signature sequence (PS00571) were identified in plant genome data bases, and a cDNA was isolated by reverse transcriptase-PCR using Arabidopsis genome sequences to develop appropriate oligonucleotide primers. The cDNA was sequenced and predicted to encode a protein of 607 amino acids with 37% identity to rat FAAH within the amidase signature domain (18% over the entire length). Residues determined to be important for FAAH catalysis were conserved between the Arabidopsis and rat protein sequences. In addition, a single transmembrane domain near the N terminus was predicted in the Arabidopsis protein sequence, similar to that of the rat FAAH protein. The putative plant FAAH cDNA was expressed as an epitope/His-tagged fusion protein in E. coli and solubilized from cell lysates in the nonionic detergent, dodecyl maltoside. Affinity-purified recombinant protein was indeed active in hydrolyzing a variety of naturally occurring N-acylethanolamine types. Kinetic parameters and inhibition data for the recombinant Arabidopsis protein were consistent with these properties of the enzyme activity characterized previously in plant and animal systems. Collectively these data now provide support at the molecular level for a conserved mechanism between plants and animals for the metabolism of NAEs.

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This research was originally published in The Journal of Biological Chemistry. Rhidaya Shrestha, Richard A. Dixon, and Kent D. Chapman. Molecular Identification of a Functional Homologue of the Mammalian Fatty Acid Amide Hydrolase in Arabidopsis thaliana. The Journal of Biological Chemistry. 2004. Vol: 278, pp. 34990-34997. © the American Society for Biochemistry and Molecular Biology.

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  • The Journal of Biological Chemistry, 2003, Rockville: American Society for BIochemistry and Molecular Biology, pp. 34990-34997

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  • Publication Title: The Journal of Biological Chemistry
  • Volume: 278
  • Issue: 37
  • Page Start: 34990
  • Page End: 34997
  • Pages: 8
  • Peer Reviewed: Yes

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  • June 24, 2003

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  • Dec. 19, 2014, 4:08 p.m.

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  • Oct. 6, 2022, 10:51 a.m.

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Thumbnail image of item number 1 in: 'Molecular Identification of a Functional Homologue of the Mammalian Fatty Acid Amide Hydrolase in Arabidopsis thaliana'.
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Shrestha, Rhidaya; Dixon, R. A. & Chapman, Kent D. Molecular Identification of a Functional Homologue of the Mammalian Fatty Acid Amide Hydrolase in Arabidopsis thaliana, article, June 24, 2003; [Rockville, Maryland]. (https://digital.library.unt.edu/ark:/67531/metadc485928/: accessed July 18, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT College of Arts and Sciences.

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