Analysis and expression of the cotton gene for the D-12 fatty acid desaturases 2-4 (FAD2-4)

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A genomic clone containing a 16.9-kb segment of cotton DNA was found to encompass a D-12 fatty acid desaturases (FAD2-4) gene. The FAD2-4 gene has a single, large intron of 2,780 bp in its 5'-untranslated region, just 12 bp upstream from the ATG initiation codon of the FAD2-4 opening reading frame. A number of prospective promoter elements, including several light-responsive sequences, occur in the 5'-flanking region. The coding region of the gene is 1155 bp with no introns, and would encode a FAD2-4 polypeptide of 384 amino acids. The putative protein had four membrane-spanning helices, hallmarks of an integral membrane … continued below

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Park, Stacy J. August 2003.

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  • Park, Stacy J.

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A genomic clone containing a 16.9-kb segment of cotton DNA was found to encompass a D-12 fatty acid desaturases (FAD2-4) gene. The FAD2-4 gene has a single, large intron of 2,780 bp in its 5'-untranslated region, just 12 bp upstream from the ATG initiation codon of the FAD2-4 opening reading frame. A number of prospective promoter elements, including several light-responsive sequences, occur in the 5'-flanking region. The coding region of the gene is 1155 bp with no introns, and would encode a FAD2-4 polypeptide of 384 amino acids. The putative protein had four membrane-spanning helices, hallmarks of an integral membrane protein, and would probably be located in the endoplasmic reticulum. The FAD2-4 gene is indeed a functional gene, since yeast cells transformed with a plasmid containing the coding region of the gene synthesize an appreciable amount of linoleic acid (18:2), not normally made in wild-type yeast cells. The FAD2-4 gene has many structural similarities to the cotton FAD2-3 gene that was also analyzed in this laboratory.

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  • August 2003

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  • Feb. 15, 2008, 2:52 p.m.

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Thumbnail image of item number 1 in: 'Analysis and expression of the cotton gene for the D-12 fatty acid desaturases 2-4 (FAD2-4)'.
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Park, Stacy J. Analysis and expression of the cotton gene for the D-12 fatty acid desaturases 2-4 (FAD2-4), thesis, August 2003; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc4299/: accessed July 5, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .

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