Impact of Anti-S2 Peptides on a Variety of Muscle Myosin S2 Isoforms and Hypertrophic Cardiomyopathy Mutants Revealed by Fluorescence Resonance Energy Transfer and Gravitational Force Spectroscopy Page: IV

                                
TABLE OF CONTENTS
Page
ACKNOWLEDGEMENT ............................................................................................................. iii
LIST OF TABLES......................................................................................................................... vi
LIST OF FIGURES ...................................................................................................................... vii
ABBREVIATIONS ........................................................................................................................ x

CHAPTER
1.1
1.2
1.3
1.4
1.5
1.6

1. INTRODUCTION                 ................................................................................................1
Muscle and Sarcomere .........................................................................................1
Myosin .................................................................................................................... 3
Actin........................................................................................................................ 4
Muscle Contraction            and Its Regulation................................................................5
1.4.1      Cardiac and Skeletal Muscles ..................................................................5
1.4.1      Smooth Muscle ......................................................................................        7
Familial Hypertrophic Cardiomyopathy ..............................................................7
Hypothesis and Testing.......................................................................................10
1.6.1 Different Concentrations of the Stabilizer and the Destabilizer Peptide
Changes the Distance between Two Helix of Coiled Coil Myosin S2 in
Muscle Myosin S2 Isoforms and FHC Mutants (E924K & AE930) ........ 12
1.6.2 The Stabilizer Peptide is Specific to Myosin S2 Coiled Coil and Stabilizes
the Myosin S2 Coiled Coil thus Decreasing the Amount of Force
Produced and The Destabilizer Peptide is Specific to Myosin S2 Coiled
Coil and Destabilizes the Myosin S2 Coiled Coil thus increasing the
Amount of Force Produced.....................................................................13

CHAPTER            2. M    ATERIALS            AND       M  ETHOD.........................................................................
2.1         M   aterials    ..........................................................................................................  .
2.1.1       M   yosin    .................................................................................................  .
2.1.2       A  ctin  ....................................................................................................  .
2.1.3       Human        a-cardiac M        yosin     S2   Peptide ..................................................
2.1.4       Human a-cardiac Mutant Myosin S2 Peptide (AE930)................
2.1.5       Human a-cardiac Mutant Myosin S2 Peptide (E924K).................
2.1.6       Human        Skeletal M       yosin     S2   Peptide ....................................................
2.1.7       Human        Smooth       M   yosin    S2    Peptide.....................................................

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