Quantitative precision measurements can be made of the optical properties of individual mammalian cells using flow microphotometry. Suspended cells pass through a special flow chamber where they are lined up for exposure to blue light from an argon-ion laser. As each cell crosses the laser beam, it produces one or more optical pulses of a duration equal to cell transit time across the beam. These pulses are detected, amplified, and analyzed using the techniques of gamma ray spectroscopy. Quantitative DNA distributions made it possible to distinguish tumor cells from normal cells as well as to assay for radiation effects on …
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Los Alamos Scientific Lab., N.Mex. (USA)
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New Mexico
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Quantitative precision measurements can be made of the optical properties of individual mammalian cells using flow microphotometry. Suspended cells pass through a special flow chamber where they are lined up for exposure to blue light from an argon-ion laser. As each cell crosses the laser beam, it produces one or more optical pulses of a duration equal to cell transit time across the beam. These pulses are detected, amplified, and analyzed using the techniques of gamma ray spectroscopy. Quantitative DNA distributions made it possible to distinguish tumor cells from normal cells as well as to assay for radiation effects on tumor cells subjected to x and gamma radiation. (HLW)
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Mullaney, P. F.; Steinkamp, J. A.; Crissman, H. A.; Cram, L. S.; Crowell, J. M.; Salzman, G. C. et al.Laser flow microphotometry for rapid analysis and sorting of mammalian cells. [X and gamma radiation],
article,
January 1, 1976;
New Mexico.
(https://digital.library.unt.edu/ark:/67531/metadc1442827/:
accessed July 16, 2024),
University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu;
crediting UNT Libraries Government Documents Department.
