Fluorescence polarization and pulse width analysis of chromosomes by a flow system. [Hamsters]

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Isolated Chinese hamster chromosomes have been analyzed using a multiparameter computer-controlled cell sorter (MACCS) to obtain information about unique properties of individual chromosomes. Parameters other than DNA content were sought that would further aid in distinguishing among chromosomes. The polarized emission of Hoechst 33342 was measured for each class of chromosomes identified by a distinct peak. The emission anisotropy values for every chromosome class was a constant (EA = 0.30) with chromosome size and DNA content. Pulse width was found to be a good parameter for resolving chromosomes, presumably according to arm length.

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30 pages

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Cram, L.S.; Ardnt-Jovin, D.J.; Grimwade, B.G. & Jovin, T.M. January 1, 1978.

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Description

Isolated Chinese hamster chromosomes have been analyzed using a multiparameter computer-controlled cell sorter (MACCS) to obtain information about unique properties of individual chromosomes. Parameters other than DNA content were sought that would further aid in distinguishing among chromosomes. The polarized emission of Hoechst 33342 was measured for each class of chromosomes identified by a distinct peak. The emission anisotropy values for every chromosome class was a constant (EA = 0.30) with chromosome size and DNA content. Pulse width was found to be a good parameter for resolving chromosomes, presumably according to arm length.

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30 pages

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Dep. NTIS, PC A03/MF A01.

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  • 6. conference on automated cytology, Elmau, F.R. Germany, 23 Apr 1978

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  • Report No.: LA-UR-78-2276
  • Report No.: CONF-780488-6
  • Grant Number: W-7405-ENG-36
  • Office of Scientific & Technical Information Report Number: 6543347
  • Archival Resource Key: ark:/67531/metadc1191941

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  • January 1, 1978

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  • July 3, 2018, 8:14 a.m.

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  • Feb. 2, 2021, 2:26 p.m.

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Cram, L.S.; Ardnt-Jovin, D.J.; Grimwade, B.G. & Jovin, T.M. Fluorescence polarization and pulse width analysis of chromosomes by a flow system. [Hamsters], article, January 1, 1978; New Mexico. (https://digital.library.unt.edu/ark:/67531/metadc1191941/: accessed July 16, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.

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