Flow cytometric analysis of respiratory tract cells exposed to oil shale and silica particulates. [Hamsters]

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Flow cytometric techniques were used to measure the cytological and biochemical damage to respiratory tract cells in animals exposed to particulates. Hamsters were exposed to raw and spent oil shale particulates and silica by intratracheal instillation. Exfoliated lung cells were obtained by sacrificing the animals and lavaging the respiratory tract posterior to the trachea with saline. Cell samples were fixed in ethanol and stained with mithramycin for fluorescence analysis of DNA content. DNA content distributions from hamsters exposed to spent oil shale and silica particulates showed atypical changes 28 to 35 days later. Cell counts and total numbers of macrophages, … continued below

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19 pages

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Steinkamp, J. A. & Wilson, J. S. January 1, 1979.

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Flow cytometric techniques were used to measure the cytological and biochemical damage to respiratory tract cells in animals exposed to particulates. Hamsters were exposed to raw and spent oil shale particulates and silica by intratracheal instillation. Exfoliated lung cells were obtained by sacrificing the animals and lavaging the respiratory tract posterior to the trachea with saline. Cell samples were fixed in ethanol and stained with mithramycin for fluorescence analysis of DNA content. DNA content distributions from hamsters exposed to spent oil shale and silica particulates showed atypical changes 28 to 35 days later. Cell counts and total numbers of macrophages, leukocytes, and epithelial cells in the lavage fluid also showed marked changes related to time after exposure.

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19 pages

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Dep. NTIS, PC A02/MF A01.

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  • 19. annual Hanford life sciences symposium, Richland, WA, USA, 22 Oct 1979

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  • Report No.: LA-UR-79-2737
  • Report No.: CONF-791002-1
  • Grant Number: W-7405-ENG-36
  • Office of Scientific & Technical Information Report Number: 5774426
  • Archival Resource Key: ark:/67531/metadc1100176

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  • January 1, 1979

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  • Feb. 18, 2018, 3:59 p.m.

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  • May 24, 2021, 6:55 p.m.

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Steinkamp, J. A. & Wilson, J. S. Flow cytometric analysis of respiratory tract cells exposed to oil shale and silica particulates. [Hamsters], article, January 1, 1979; New Mexico. (https://digital.library.unt.edu/ark:/67531/metadc1100176/: accessed July 16, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.

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