Molecular mechanism by which cyclic amp regulates myocardial contractility Page: 97 of 153
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65
FIGURE 1
Elution profile from the Sepnadex G-75 column. Cardiac
microsomes were suspended at 7 mg/ml in 0.25 M sucrose. 2 yl of
0.1% DOC was added per 7 mg protein. The microsomes were
centrifuged at 100,000 x g for 30 minutes. The supernatants were
concentrated and approximately 2.7 mg of protein was applied to a
Sephadex G-75 (fine) column (1.5 cm x 1.5 m) equilibrated with 10
mM tris (pH 7.8), 0.04% DOC, 0.02% NaN^. The absorbance of the
eluant was monitored at 280 nm. Each fraction was 4.7 ml.
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Bidlack, J. M. Molecular mechanism by which cyclic amp regulates myocardial contractility, report, January 1, 1979; United States. (https://digital.library.unt.edu/ark:/67531/metadc1085147/m1/97/?rotate=270: accessed July 18, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.