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The bioenergetics of salt tolerance

Description: The aim of this project was to try to understand the adaptive mechanisms that organisms develop in order to respond to a sudden transformation in their environment to a salt shock.'' To study this problem we used a fresh water oxygenic photosynthetic cyanobacterium known as Synecoccus 6311. This organism suffers injury after this sudden exposure to high concentrations of sodium chloride equivalent to or even higher than that in sea water. Yet they are able to re-establish their photosynthetic a… more
Date: January 1, 1991
Creator: Packer, L.
Partner: UNT Libraries Government Documents Department
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MSU-DOE Plant Research Laboratory

Description: This document is the compiled progress reports of research funded through the Michigan State University/Department of Energy Plant Research Laboratory. Fourteen reports are included, covering the molecular basis of plant/microbe symbiosis, cell wall biosynthesis and proteins, gene expression, stress responses, plant hormone biosynthesis, interactions between the nuclear and organelle genomes, sensory transduction and tropisms, intracellular sorting and trafficking, regulation of lipid metabolis… more
Date: January 1, 1991
Partner: UNT Libraries Government Documents Department
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Interdisciplinary research and training program in the plant sciences

Description: This document is the compiled progress reports from the Interdisciplinary Research and Training Program in the Plant Sciences funded through the MSU-DOE Plant Research Laboratory. Fourteen reports are included, covering topics such as the molecular basis of plant/microbe symbiosis, cell wall proteins and assembly, gene expression, stress responses, growth regulator biosynthesis, interaction between nuclear and organelle genomes, sensory transduction and tropisms, intracellular sorting and membr… more
Date: January 1, 1991
Creator: Wolk, C.P.
Partner: UNT Libraries Government Documents Department
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Characterization of a 1,4-. beta. -D-glucan synthase from Dictyostelium discoideum

Description: Various aspects of research concerning Dictyostelium discoideum are presented. The initial focus of this project was upon: the characterization of potential probes for the cellulose synthase (antibody and nucleic acid), the determination of the cultural induction conditions of cellulose synthesis, the solubilization of the enzyme activity, the development of a non-inhibitory disruption buffer, the generation and isolation of mutant strains deficient in cellulose synthesis, and the development o… more
Date: January 15, 1992
Creator: Blanton, R.L.
Partner: UNT Libraries Government Documents Department
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Energy transfer and utilization in biological systems. Final report, September 1, 1967--September 15, 1976. [Fluorescence studies of photosynthesis]

Description: In fluorescence studies of the photosynthetic process it was shown that the photosynthetic rate and light loss via chlorophyll fluorescence are strictly complementary. The exciplex state of matter responsible for most photochemistry was discovered and a wide variety of information about the state gained from studies of indole exciplexes. In addition to the nature of the bonding, degree of stability and general excited state behavior of indole exciplexes, their ability to lose electrons in a hig… more
Date: March 1, 1978
Creator: Lumry, R.W.
Partner: UNT Libraries Government Documents Department
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Picosecond electron transfer in diporphyrin models of Photosystem II of green plants

Description: Green plants and photosynthetic bacteria efficiently transform the energy of an absorbed photon into redox products. Current in vivo and in vitro studies on Photosystem II (PS II) suggest the electron donor is a chlorophyll monomer, ligated to produce the high oxidation potential of P680, and the electron acceptor is pheophytin, a metal-free chlorophyll. This study probes the behavior of this PS II model in solvents of high dielectric constant and tests the sensitivity of its charge transfer re… more
Date: January 1, 1980
Creator: Netzel, T L; Fujita, I; Wang, C B & Fajer, J
Partner: UNT Libraries Government Documents Department
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Report on picosecond studies of electron transfer in photosynthetic models

Description: Considerable spectroscopic work on reaction centers 8RC) from photosynthetic bacteria and on photosystem I (PSI) particles from green plants has established that the initial photochemical step in these systems is the subnanosecond tranfer of an electron resulting in the creation of an oxidized donor and a reduced acceptor. For both of these systems the electron donor is a dimer. The acceptor for bacterial RC's is bacteriopheophytin, a metal-free bacteriochlorophyll. The acceptor for PSI is… more
Date: January 1, 1980
Creator: Netzel, T L; Bucks, R R; Boxer, S G & Fujita, I
Partner: UNT Libraries Government Documents Department
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Investigation of the Triplet State of Chlorophylls. Technical Progress Report, May 1, 1979-April 30, 1980

Description: The triplet state of chlorophyll has been utilized as a nondestructive probe into the structural and dynamical nature of the photosynthetic apparatus, both in vitro and in isolated in vivo subunits. During the past year, using zero-field triplet state optically detected magnetic resonance spectroscopy, the research program has examined triplet energy transfer between photosynthetic pigments in solution; has isolated and studied subchloroplast particles enriched with photo-system I and the light… more
Date: January 1, 1980
Creator: Clarke, R. H.
Partner: UNT Libraries Government Documents Department
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(Calcium gating of proton fluxes in chloroplasts)

Description: Work supported by this grant has been aimed at better understanding the still-mysterious phenomenon of sequestered proton gradients which strong evidence suggests can energize ATP formation in chloroplast organelle membranes. Results from several laboratories support the notion that chloroplasts can couple the energy of H{sup +} gradients. Research in may lab has, since 1983, been deeply involved in a two-pronged approach, (A) identifying and quantitating sequestered proton buffering groups and… more
Date: January 1, 1991
Creator: Dilley, R. A.
Partner: UNT Libraries Government Documents Department
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The active site of ribulose-bisphosphate carboxylase/oxygenase

Description: The active site of ribulose-bisphosphate carboxylase/oxygenase requires interacting domains of adjacent, identical subunits. Most active-site residues are located within the loop regions of an eight-stranded {beta}/{alpha}-barrel which constitutes the larger C-terminal domain; additional key residues are located within a segment of the smaller N-terminal domain which partially covers the mouth of the barrel. Site-directed mutagenesis of the gene encoding the enzyme from Rhodospirillum rubrum ha… more
Date: January 1, 1991
Creator: Hartman, F. C.
Partner: UNT Libraries Government Documents Department
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Biochemical endpoints of glucocorticoid hormone action

Description: Both the rapidly evolving metabolic effects of glucocorticoids and the more slowly developing lethal actions appear to be initiated via the synthesis of new mRNAs and proteins. The chronic suppression of cell growth may be the consequence of suppression of overall rates of protein synthesis (and probably RNA and DNA synthesis as well) that in turn may represent the cellular response to the small changes in ratios of adenine nucleotides that result from the suppression of oxidative ATP productio… more
Date: January 1, 1978
Creator: Young, D. A.; Nicholson, M. L.; Guyette, W. A.; Giddings, S. J.; Mendelsohn, S. L.; Nordeen, S. K. et al.
Partner: UNT Libraries Government Documents Department
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New prodrugs based on phospholipid-nucleoside conjugates

Description: A method is described for the preparation of defined, isomerically pure phospholipid-nucleoside conjugates as a prodrug in which the drug (araC) is attached to the phospholipid by a monophosphate linkage. Key intermediates in the process involve selective blocking and deblocking of the nucleoside derivative. These particular monophosphate-linked derivatives represent a new class of prodrug, which are useful by themselves or in combination with diphosphate linked derivatives. Several new composi… more
Date: February 3, 1982
Creator: MacCoss, M.
Partner: UNT Libraries Government Documents Department
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Identification of zein structural genes in the maize genome. [Maize proteins]

Description: Zein is the major storage protein in maize endosperm and is deficient in the amino acids lysine and tryptophan, the amino acids generally limiting in cereal grains. If the two subunits of zein were each encoded by a single gene, it is possible to envisage a program in which altered gene products containing these two amino acids could be selected. Since zein constitutes such a large proportion of the total protein, substitutions of rare amino acids into zein will be reflected in the overall amin… more
Date: January 1, 1978
Creator: Burr, B
Partner: UNT Libraries Government Documents Department
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Investigation of the Triplet State of Chlorophylls. Progress Report, May 1, 1980-April 30, 1981

Description: The triplet state of chlorophyll has been utilized as a nondestructive probe into the structural and dynamical nature of the photosynthetic apparatus. During the past year, using zero-field triplet state optically detected magnetic resonance spectroscopy, chlorophyll aggregates were examined in both their natural environment in vivo and in model complexes in vitro. Research efforts have focused on subchloroplast particles extracted from green plants, on chlorophyll monolayers, and on isolated c… more
Date: December 22, 1980
Creator: Clarke, R. H.
Partner: UNT Libraries Government Documents Department
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Measurements of metabolically active inorganic phosphate in plants growing in natural and agronomic settings and under water stress. [Stromal Phosphate]

Description: At high rates of photosynthesis, the conflicting requirements of adenosine triphosphate (ATP) synthesis for phosphate and starch and sucrose synthesis for low phosphate, may limit the overall rate of photosynthesis. This is called feedback limitation of photosynthesis. A nonaqueous fractionation technique was used to measure stromal phosphate levels without contamination from vacuolar phosphate. Under normal conditions the stromal phosphate level was found to be 7mM. Under feedback limited phot… more
Date: January 1, 1988
Creator: Sharkey, T.D.
Partner: UNT Libraries Government Documents Department
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Photochemistry and enzymology of photosynthesis. Annual progress report, May 1, 1975--April 30, 1976

Description: Work during the reporting period can be separated into four main topics. Highlights include oxygen evolution, proton transport, acid-base induced fluorescence changes, and mass spectrometer studies. Under some conditions we have observed the apparent transition of S/sub 1/ to S/sub 2/ in the dark. Redox titrations showed that this transition has a midpoint potential of 400 mV at pH 7, and a pH dependence of 60 mV per pH unit. The proton uptake and release that apparently accompany the reduction… more
Date: April 1, 1976
Creator: Kok, B.; Fowler, C. F.; Radmer, R. J. & Hardt, H. H.
Partner: UNT Libraries Government Documents Department
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Comprehensive report, June 1973--June 1976

Description: Progress is reported on the following research projects: the molecular basis of disease resistance; synthesis of phytoalexins by plants; host-pathogen interactions; structure and function of primary cell walls; and production of galactanase by Bacillus subtilis. (HLW)
Date: January 1, 1976
Partner: UNT Libraries Government Documents Department
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Interdisciplinary Research and Training Program in the Plant Sciences

Description: Research on plants continued. Topics include: Molecular basis of symbiotic plant-microbe interations; enzymatic mechanisms and regulation of plant cell wall biosynthesis; molecular mechanisms that regulate the expression of genes in plants; resistance of plants to environmental stress; studies on hormone biosynthesis and action; plant cell wall proteins; interaction of nuclear and organelle genomes; sensor transduction in plants; molecular mechanisms of trafficking in the plant cell; regulation… more
Date: January 1, 1992
Creator: Wolk, C. P.
Partner: UNT Libraries Government Documents Department
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