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MSU-DOE Plant Research Laboratory

Description: This document is the compiled progress reports of research funded through the Michigan State University/Department of Energy Plant Research Laboratory. Fourteen reports are included, covering the molecular basis of plant/microbe symbiosis, cell wall biosynthesis and proteins, gene expression, stress responses, plant hormone biosynthesis, interactions between the nuclear and organelle genomes, sensory transduction and tropisms, intracellular sorting and trafficking, regulation of lipid metabolis… more
Date: January 1, 1991
Partner: UNT Libraries Government Documents Department
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Interdisciplinary research and training program in the plant sciences

Description: This document is the compiled progress reports from the Interdisciplinary Research and Training Program in the Plant Sciences funded through the MSU-DOE Plant Research Laboratory. Fourteen reports are included, covering topics such as the molecular basis of plant/microbe symbiosis, cell wall proteins and assembly, gene expression, stress responses, growth regulator biosynthesis, interaction between nuclear and organelle genomes, sensory transduction and tropisms, intracellular sorting and membr… more
Date: January 1, 1991
Creator: Wolk, C.P.
Partner: UNT Libraries Government Documents Department
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Rapid regulatory control of plant cell expansion and wall relaxation

Description: The aim of this project is to elucidate the biophysical and cellular mechanisms that control plant cell expansion. At present we are attempting to characterize the kinetics of the system(s) responsible for regulatory and compensatory behavior of growing cells and tissues. This work is significantly because it indicates that biochemical loosening and biophysical stress relaxation of the wall are part of a feedback loop controlling growth. This report briefly summarizes the efforts and results of… more
Date: August 14, 1991
Creator: Cosgrove, D.J.
Partner: UNT Libraries Government Documents Department
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Physiopathology of blood platelets: a model system for studies of cell-to-cell interaction. Progress report, November 1, 1978-October 31, 1979

Description: In this report, we will limit ourselves to the detailed description of four major sections of our research done during the past year: platelet interaction with tumor cells; studies of the interaction of platelets with macrophages; interaction of platelets with vessel walls; and further studies of cyclic nucleotides on stored platelets.
Date: January 1, 1979
Creator: Baldini, M G
Partner: UNT Libraries Government Documents Department
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Studies on the control of cell wall extension. Yearly progress report, September 1, 1978-August 31, 1979

Description: Research has been centered around the question as to how plant cell enlargement is controlled and regulated at the cellular level. Progress is reported on the following projects: proton permeability of plant cuticles; the control of osmoregulation in Avena coleoptiles; an analysis of the acid-extension curves. (ACR)
Date: January 1, 1979
Creator: Cleland, R. E.
Partner: UNT Libraries Government Documents Department
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Regulating Intracellular Calcium in Plants: From Molecular Genetics to Physiology

Description: To grow, develop, adapt, and reproduce, plants have evolved mechanisms to regulate the uptake, translocation and sorting of calcium ions into different cells and subcellular compartments. Yet how plants accomplish this remarkable feat is still poorly understood. The spatial and temporal changes in intracellular [Ca2+] during growth and during responses to hormonal and environmental stimuli indicate that Ca2+ influx and efflux transporters are diverse and tightly regulated in plants. The specifi… more
Date: June 22, 2008
Creator: Sze, Heven
Partner: UNT Libraries Government Documents Department
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Magnetophoretic bead trapping in a high-flowrate biological detection system.

Description: This report contains the summary of the 'Magnetophoretic Bead Trapping in a High-Flowrate Biological Detection System' LDRD project 74795. The objective of this project is to develop a novel biodetection system for high-throughput sample analysis. The chief application of this system is in detection of very low concentrations of target molecules from a complex liquid solution containing many different constituents--some of which may interfere with identification of the target molecule. The syst… more
Date: March 1, 2005
Creator: Galambos, Paul C.; Hopkins, Matthew Morgan; Rahimian, Kamayar; Martin, James Ellis; Anderson, G. Ronald; Clem, Paul Gilbert et al.
Partner: UNT Libraries Government Documents Department
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Genetic analysis of the regulation of TCH gene expression, Final Report

Description: The Arabidopsis TCH genes, originally isolated as a consequence of their upregulation in response to the mechanical stimulus of touch, are also upregulated by a variety of seemingly disparate environmental and hormonal stimuli. To gain insight into the complexities of TCH gene regulation, a number of approaches were taken. Regulatory elements responsible for regulation were identified and characteristics of the regulation were evaluated. Reporter genes were used to monitor expression localizati… more
Date: October 28, 2008
Creator: Braam, Janet
Partner: UNT Libraries Government Documents Department
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Understanding amine catalyzed silica polymerization : diatoms as bioarchitects.

Description: Current state-of-the-art biomimetic methodologies employed worldwide for the realization of self-assembled nanomaterials are adequate for certain unique applications, but a major breakthrough is needed if these nanomaterials are to obtain their true promise and potential. These routes typically utilize a 'top-down' approach in terms of controlling the nucleation, growth, and deposition of structured nanomaterials. Most of these techniques are inherently limited to primarily 2D and simple 3D str… more
Date: October 1, 2007
Creator: Spoerke, Erik David; Aubry, Sylvie; Lane, Pamela; Robinson, David B; Bauer, Christina A.; Zendejas, Frank et al.
Partner: UNT Libraries Government Documents Department
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High Thermal Conductivity Carbon Foam used for the Thermal Management of Engine Oil

Description: The need for maintaining a lower specific engine oil temperature is essential in enhancing the longevity of the oil and of the engine and its components. By decreasing the engine oil temperature the oil is able to perform its job more efficiently. It is proposed to use the carbon foam, with its exceptional thermal management capabilities, to aid in reducing and stabilizing the engine oil temperature during steady state operation. Also, it is possible to use the carbon foam to heat the engine oi… more
Date: February 2, 2006
Creator: Ott, R. D.; McMillan, A. D. & Choudhury, A.
Partner: UNT Libraries Government Documents Department
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Final Report: DOE/ID/14215

Description: The proposed straw separation system developed in the research project harvests the large internode sections of the straw which has the greater potential as a feedstock for lignocellulosic ethanol production while leaving the chaff and nodes in the field. This strategy ensures sustainable agriculture by preventing the depletion of soil minerals, and it restores organic matter to the soil in amounts and particle sizes that accommodate farmers’ needs to keep tillage and fertilizer costs low. A to… more
Date: August 18, 2008
Creator: Bryden, Kenneth; Hess, J. Richard; Ulrich, Thomas & Zemetra, Robert
Partner: UNT Libraries Government Documents Department
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Comparative genome analysis of Bacillus cereus group genomes withBacillus subtilis

Description: Genome features of the Bacillus cereus group genomes (representative strains of Bacillus cereus, Bacillus anthracis and Bacillus thuringiensis sub spp israelensis) were analyzed and compared with the Bacillus subtilis genome. A core set of 1,381 protein families among the four Bacillus genomes, with an additional set of 933 families common to the B. cereus group, was identified. Differences in signal transduction pathways, membrane transporters, cell surface structures, cell wall, and S-layer p… more
Date: September 14, 2005
Creator: Anderson, Iain; Sorokin, Alexei; Kapatral, Vinayak; Reznik, Gary; Bhattacharya, Anamitra; Mikhailova, Natalia et al.
Partner: UNT Libraries Government Documents Department
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Genetic Augmentation of Syringyl Lignin in Low-lignin Aspen Trees, Final Report

Description: As a polysaccharide-encrusting component, lignin is critical to cell wall integrity and plant growth but also hinders recovery of cellulose fibers during the wood pulping process. To improve pulping efficiency, it is highly desirable to genetically modify lignin content and/or structure in pulpwood species to maximize pulp yields with minimal energy consumption and environmental impact. This project aimed to genetically augment the syringyl-to-guaiacyl lignin ratio in low-lignin transgenic aspe… more
Date: November 10, 2004
Creator: Tsai, Chung-Jui; Davis, Mark F. & Chiang, Vincent L.
Partner: UNT Libraries Government Documents Department
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Summer Internship Summary Paper

Description: The visualization of biological molecules and assemblies can provide enormous insight into protein structure-function relationships, as well as practical applications to fields such as microbial forensics. Atomic force microscopy (AFM) is a powerful tool for imaging soft biomaterials such as cells, spores, and proteins with nanometer resolution. The goal of this work is to understand pathogen architecture and its application to microbial forensics and medicine. Initial work has focused on imagi… more
Date: August 8, 2006
Creator: Hill, H D
Partner: UNT Libraries Government Documents Department
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An In-situ method for the study of strain broadening usingsynchrotronx-ray diffraction

Description: A tensonometer for stretching metal foils has beenconstructed for the study of strain broadening in x-ray diffraction lineprofiles. This device, which is designed for use on the powderdiffractometer in Station 2.3 at Daresbury Laboratory, allows in-situmeasurements to be performed on samples under stress. It can be used fordata collection in either transmission or reflection modes using eithersymmetric or asymmetric diffraction geometries. As a test case,measurements were carried out on a 18mum… more
Date: December 15, 2006
Creator: Tang, Chiu C.; Lynch, Peter A.; Cheary, Robert W. & Clark, Simon M.
Partner: UNT Libraries Government Documents Department
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METHANE INCORPORATION BY PROCARYOTIC PHOTOSYNTHETICMICROORGANISMS

Description: The procaryotic photosynthetic microorganisms Anacystis nidulans, Nostoc and Rhodospirillum rubrum have cell walls and membranes that are resistant to the solution of methane in their lipid components and intracellular fluids. But Anacystis nidulans, possesses a limited bioxidant system, a portion of which may be extracellularly secreted, which rapidly oxidizes methane to carbon dioxide. Small C{sup 14} activities derived from CH{sub 4} in excess of experimental error are detected in all the ma… more
Date: August 1, 1970
Creator: Norton, Charles J.; Kirk, Martha & Calvin, Melvin
Partner: UNT Libraries Government Documents Department
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An Integrated Functional Genomics Consortium to Increase Carbon Sequestration in Poplars: Optimizing Aboveground Carbon Gain

Description: This project used gene expression patterns from two forest Free-Air CO2 Enrichment (FACE) experiments (Aspen FACE in northern Wisconsin and POPFACE in Italy) to examine ways to increase the aboveground carbon sequestration potential of poplars (Populus). The aim was to use patterns of global gene expression to identify candidate genes for increased carbon sequestration. Gene expression studies were linked to physiological measurements in order to elucidate bottlenecks in carbon acquisition in t… more
Date: February 17, 2009
Creator: Karnosky, David F (deceased); Podila, G Krishna & Burton, Andrew J (for DF Karnosky)
Partner: UNT Libraries Government Documents Department
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Role of turgor pressure and solute transport in plant cell growth: Progress report

Description: Plant cell expansion requires coordinationion of three distinct processes: wall relaxation and synthesis, water uptake, and solute uptake. Wall relaxation reduces cell turgor pressure and thereby generates the reduced water for water potential needed uptake. Our studies with pea (Pisum sativum L.) and soybean (Glycine max Merr.) seedlings have shown that water uptake is rapid and is not a major control point for growth. Our current focus is on the processes of wall relaxation and solute transpo… more
Date: October 15, 1987
Creator: Cosgrove, D.J.
Partner: UNT Libraries Government Documents Department
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Raman microprobe investigation of molecular structure and organization in the native state of woody tissue. Progress report, April 1, 1982-July 31, 1983

Description: The program has focused on installation of the Raman microprobe system and making it operational, on demonstrating the feasibility of the experimental approach, and on exploratory studies of molecular organization and structural variability in selected samples of plant tissue. The key findings are: (1) it is possible to acquire significant Raman spectral information from domains that are 1 to 3 ..mu..m in diameter in sections of plant cell walls; (2) polarized spectra are sensitive to orientati… more
Date: August 1, 1983
Creator: Atalla, R. H.
Partner: UNT Libraries Government Documents Department
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Studies on the control of plant cell enlargement by cellular parameters

Description: The overall goal of this grant was to conduct research that would illuminate the mechanisms by which the plant hormone auxin induces plant cells to enlarge. A large body of knowledge already existed concerning this process. To begin with, it had been demonstrated, primarily by this laboratory during previous grant periods, that the effect of auxin is to cause a loosening of the cell wall, which is followed by turgor-driven wall expansion and water uptake. The wall contains a series of load-bear… more
Date: December 6, 1991
Creator: Cleland, R.E.
Partner: UNT Libraries Government Documents Department
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[Carbon and Hydrogen Metabolism of Green Algae in Light and Dark]

Description: The focus of this project was the elucidation of anaerobic metabolism in ecuaryotic green algae, chlamydomonas reinhardii. Chlamydomonas is a versatile organism that can grow under disparate conditions such as fresh water lakes and sewage ponds. The cell an photoassimilate CO{sub 2} aerobically and anaerobically, the latter after adaptation'' to a hydrogen metabolism. It can recall the knallgas or oxyhydrogen reaction and utilize hydrogen the simplest of all reducing agents for the dark assimil… more
Date: January 1, 1990
Partner: UNT Libraries Government Documents Department
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[Hydroxyproline: Rich glycoproteins of the plant and cell wall]

Description: Since xylem tissue includes the main cell types which are lignified, we are interested in gene expression of glycine-rich proteins and proline-rich proteins, and other proteins which are involved in secondary cell wall thickening during xylogenesis. Since the main feature of xylogenesis is the deposition of additional wall components, study of the mechanism of xylogenesis will greatly advance our knowledge of the synthesis and assembly of wall macromolecules. We are using the in vitro xylogenes… more
Date: January 1, 1993
Creator: Varner, J.E.
Partner: UNT Libraries Government Documents Department
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Role of proteolytic enzymes in degradation of plant tissues

Description: Strain SR 394 of Erwinia carotovora (Ecc) produced proteases constitutively in all media tested. Growth of Ecc and production of protease were enhanced significantly by the presence of poetic materials and/or plant call walls in the test media. After electrofocusing, one major and one minor protease bands, at PI 4.8 and PI 5.1, respectively, were detected. Only one band of 43 kDa was detected on SDS gels. Only one protease band was detected in SDS gels of infected plant extracts. This protease … more
Date: January 1, 1991
Creator: Lewosz, J.; Kelman, A. & Sequeira, L.
Partner: UNT Libraries Government Documents Department
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