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Single sample extraction and HPLC processing for quantification of NAD and NADH levels in Saccharomyces cerevisiae

Description: A robust redox extraction protocol for quantitative and reproducible metabolite isolation and recovery has been developed for simultaneous measurement of nicotinamide adenine dinucleotide (NAD) and its reduced form, NADH, from Saccharomyces cerevisiae. Following culture in liquid media, approximately 10{sup 8} yeast cells were harvested by centrifugation and then lysed under non-oxidizing conditions by bead blasting in ice-cold, nitrogen-saturated 50-mM ammonium acetate. To enable protein denat… more
Date: January 10, 2008
Creator: Sporty, J; Kabir, M M; Turteltaub, K; Ognibene, T; Lin, S & Bench, G
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Prognostic breast cancer signature identified from 3D culture model accurately predicts clinical outcome across independent datasets

Description: One of the major tenets in breast cancer research is that early detection is vital for patient survival by increasing treatment options. To that end, we have previously used a novel unsupervised approach to identify a set of genes whose expression predicts prognosis of breast cancer patients. The predictive genes were selected in a well-defined three dimensional (3D) cell culture model of non-malignant human mammary epithelial cell morphogenesis as down-regulated during breast epithelial cell a… more
Date: October 20, 2008
Creator: Martin, Katherine J.; Patrick, Denis R.; Bissell, Mina J. & Fournier, Marcia V.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

The Characterization of Psychrophilic Microorganisms and their potentially useful Cold-Active Glycosidases Final Progress Report

Description: Our studies of novel, cold-loving microorganisms have focused on two distinct extreme environments. The first is an ice core sample from a 120,000 year old Greenland glacier. The results of this study are particularly exciting and have been highlighted with press releases and additional coverage. The first press release in 2004 was based on our presentation at the General Meeting of the American Society for Microbiology and was augmented by coverage of our publication (Appl. Environ. Microbiol.… more
Date: June 30, 2008
Creator: Brenchly, Jean E.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Human mammary progenitor cell fate decisions are products of interactions with combinatorial microenvironments

Description: In adult tissues, multi-potent progenitor cells are some of the most primitive members of the developmental hierarchies that maintain homeostasis. That progenitors and their more mature progeny share identical genomes, suggests that fate decisions are directed by interactions with extrinsic soluble factors, ECM, and other cells, as well as physical properties of the ECM. To understand regulation of fate decisions, therefore, would require a means of understanding carefully choreographed combina… more
Date: September 19, 2008
Creator: LaBarge, Mark A; Nelson, Celeste M; Villadsen, Rene; Fridriksdottir, Agla; Ruth, Jason R; Stampfer, Martha R et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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