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in creator/contributor: "Lawrence Berkeley National Laboratory. Life Sciences Division."  
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Coordinateendonucleolytic 5' and 3' trimming of terminally blocked blunt DNA double-strand break ends by Artemis nuclease and DNA-dependent protein kinase

Description: Previous work showed that, in the presence of DNA-PK, Artemis slowly trims 3'-phosphoglycolate-terminated blunt ends. To examine the trimming reaction in more detail, long internally labeled DNA substrates were treated with Artemis. In the absence of DNA-PK, Artemis catalyzed extensive 5' {yields} 3' exonucleolytic resection of double-stranded DNA. This resection required a 5'-phosphate but did not require ATP, and was accompanied by endonucleolytic cleavage of the resulting 3' overhang. In the… more
Date: February 18, 2008
Creator: Povirk, Lawrence; Yannone, Steven M.; Khan, Imran S.; Zhou, Rui-Zhe; Zhou, Tong; Valerie, Kristoffer et al.
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Partner: UNT Libraries Government Documents Department
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Exact Reconstruction From Uniformly Attenuated Helical Cone-Beam Projections in SPECT

Description: In recent years the development of cone-beam reconstruction algorithms has been an active research area in x-ray computed tomography (CT), and significant progress has been made in the advancement of algorithms. Theoretically exact and computationally efficient analytical algorithms can be found in the literature. However, in single photon emission computed tomography (SPECT), published cone-beam reconstruction algorithms are either approximate or involve iterative methods. The SPECT reconstruc… more
Date: December 18, 2008
Creator: Gullberg, Grant T; Huang, Qiu; You, Jiangsheng & Zeng, Gengsheng L.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Photodetectors for Scintillator Proportionality Measurement

Description: We evaluate photodetectors for use in a Compton Coincidence apparatus designed for measuring scintillator proportionality. There are many requirements placed on the photodetector in these systems, including active area, linearity, and the ability to accurately measure low light levels (which implies high quantum efficiency and high signal-to-noise ratio). Through a combination of measurement and Monte Carlo simulation, we evaluate a number of potential photodetectors, especially photomultiplier… more
Date: October 18, 2010
Creator: Moses, William W.; Choong, Woon-Seng; Hull, Giulia; Payne, Steve; Cherepy, Nerine & Valentine, J. D.
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Partner: UNT Libraries Government Documents Department
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Scintillation Properties of Eu2+-Activated Barium Fluoroiodide

Description: The scintillation properties of powders and single-crystals of BaFI doped with Eu2+ are presented. Single crystals were grown by the vertical Bridgman technique. Under optical and X-ray excitation, the samples exhibit a narrow E2+ 5d-4f transition emission centered at 405 nm. The scintillation light output is estimated to be 55,000+-5,000 photons/MeV at 662 keV with 85percent of the light decaying within 600 ns. An energyresolution of 8.5percent full width at half maximum (FWHM) has been achiev… more
Date: November 18, 2009
Creator: Gundiah, Gautam; Bourret-Courchesne, Edith; Bizarri, Gregory; Hanrahan, Stephen M.; Chaudhry, Anurag; Canning, Andrew et al.
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Partner: UNT Libraries Government Documents Department
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Phenotypic transition maps of 3D breast acini obtained by imaging-guided agent-based modeling

Description: We introduce an agent-based model of epithelial cell morphogenesis to explore the complex interplay between apoptosis, proliferation, and polarization. By varying the activity levels of these mechanisms we derived phenotypic transition maps of normal and aberrant morphogenesis. These maps identify homeostatic ranges and morphologic stability conditions. The agent-based model was parameterized and validated using novel high-content image analysis of mammary acini morphogenesis in vitro with focu… more
Date: February 18, 2011
Creator: Tang, Jonathan; Enderling, Heiko; Becker-Weimann, Sabine; Pham, Christopher; Polyzos, Aris; Chen, Chen-Yi et al.
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Partner: UNT Libraries Government Documents Department
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Overexpression of SnoN/SkiL, amplified at the 3q26.2 locus, in ovarian cancers: A role in ovarian pathogenesis

Description: High-resolution array comparative genomic hybridization of 235 serous epithelial ovarian cancers demonstrated a regional increase at 3q26.2 encompassing SnoN/SkiL, a coregulator of SMAD/TGF{beta} signaling. SnoN RNA transcripts were elevated in {approx}80% of advanced stage serous epithelial ovarian cancers. In both immortalized normal (TIOSE) and ovarian carcinoma cell lines (OVCA), SnoN RNA levels were increased by TGF{beta} stimulation and altered by LY294002 and JNK II inhibitor treatment s… more
Date: July 18, 2008
Creator: Nanjundan, Meera; Cheng, Kwai Wa; Zhang, Fan; Lahad, John; Kuo, Wen-Lin; Schmandt, Rosemarie et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

DNA repair efficiency in germ cells and early mouse embryos and consequences for radiation-induced transgenerational genomic damage

Description: Exposure to ionizing radiation and other environmental agents can affect the genomic integrity of germ cells and induce adverse health effects in the progeny. Efficient DNA repair during gametogenesis and the early embryonic cycles after fertilization is critical for preventing transmission of DNA damage to the progeny and relies on maternal factors stored in the egg before fertilization. The ability of the maternal repair machinery to repair DNA damage in both parental genomes in the fertilizi… more
Date: January 18, 2009
Creator: Marchetti, Francesco & Wyrobek, Andrew J.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Chapter 27 -- Breast Cancer Genomics, Section VI, Pathology and Biological Markers of Invasive Breast Cancer

Description: Breast cancer is predominantly a disease of the genome with cancers arising and progressing through accumulation of aberrations that alter the genome - by changing DNA sequence, copy number, and structure in ways that that contribute to diverse aspects of cancer pathophysiology. Classic examples of genomic events that contribute to breast cancer pathophysiology include inherited mutations in BRCA1, BRCA2, TP53, and CHK2 that contribute to the initiation of breast cancer, amplification of ERBB2 … more
Date: June 18, 2009
Creator: Spellman, Paul T.; Heiser, Laura & Gray, Joe W.
Item Type: Refine your search to only Book
Partner: UNT Libraries Government Documents Department
open access

Delineating Rearrangements in Single Yeast Artificial Chromosomes by Quantitative DNA Fiber Mapping

Description: Cloning of large chunks of human genomic DNA in recombinant systems such as yeast or bacterial artificial chromosomes has greatly facilitated the construction of physical maps, the positional cloning of disease genes or the preparation of patient-specific DNA probes for diagnostic purposes. For this process to work efficiently, the DNA cloning process and subsequent clone propagation need to maintain stable inserts that are neither deleted nor otherwise rearranged. Some regions of the human gen… more
Date: September 18, 2009
Creator: Weier, Heinz-Ulrich G.; Greulich-Bode, Karin M.; Wu, Jenny & Duell, Thomas
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Imaging nanoscale magnetic structures with polarized soft x-ray photons

Description: Imaging nanoscale magnetic structures and their fast dynamics is scientifically interesting and technologically of highest relevance. The combination of circularly polarized soft X-ray photons which provide a strong X-ray magnetic circular dichroism effect at characteristic X-ray absorption edges, with a high resolution soft X-ray microscope utilizing Fresnel zone plate optics allows to study in a unique way the stochastical behavior in the magnetization reversal process of thin films and the u… more
Date: January 18, 2010
Creator: Fischer, P. & Im, M.-Y.
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Partner: UNT Libraries Government Documents Department
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Low Dose Radiation Response Curves, Networks and Pathways in Human Lymphoblastoid Cells Exposed from 1 to 10 cGy of Acute Gamma Radiation

Description: We investigated the low dose dependency of the transcriptional response of human cells to characterize the shape and biological functions associated with the dose response curve and to identify common and conserved functions of low dose expressed genes across cells and tissues. Human lymphoblastoid (HL) cells from two unrelated individuals were exposed to graded doses of radiation spanning the range of 1-10 cGy were analyzed by transcriptome profiling, qPCR and bioinformatics, in comparison to … more
Date: April 18, 2011
Creator: Wyrobek, A. J.; Manohar, C. F.; Nelson, D. O.; Furtado, M. R.; Bhattacharya, M. S.; Marchetti, F. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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