UNT Libraries - 5 Matching Results

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Engineering Cardiac Organoid Vascularization via Fluid Shear Stress and Vascular-Promoting Growth Factors

Description: Cardiovascular disease (CVD) is the leading cause of death internationally. Efforts to decrease CVD death has been explored through stem cell technology, specifically organoid formation. Current cardiac organoid models lack the vascular networks for nutrient supply and maturation. In this study, pillar perfusion technology is used to fabricate cardiac organoids and induce vascularization via dynamic culturing and the addition of vascular promoting growth factors (GFs). In addition to this study… more
Date: August 2022
Creator: Huerta Gomez, Angello
open access

Engineering Whole Cell-Based Biosensors for Heavy Metal Detection Using Metalloregulatory Transcriptional Repressors of the SmtB/ArsR Family

Description: This study focuses on engineering whole cell-based biosensors for heavy metal detection. Through the exploitation of metalloregulatory proteins, fabrication of metal ion-responsive biosensors is achieved. Metalloregulatory proteins of the SmtB/ArsR family including arsenite-responsive ArsR, cadmium-responsive CadC, zinc-responsive CzrA, and nickel-responsive NmtR were evaluated as biosensor sensing modules. Characterization of these four metal sensing modules was accomplished through quantifica… more
Date: May 2022
Creator: Draeger, Alison
open access

Modeling Hypertrophic Cardiomyopathy Using Genome-Edited Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Response to Dynamic Mechanotransduction

Description: Familial hypertrophic cardiomyopathy (HCM) is a genetic disease largely caused by a mutation in myosin binding protein C (MYBPC3) and it affects about 1:500 population leading to arrhythmic sudden death, heart failure, and atrial fibrillation. MYBPC3 activates calcium-induced actin-myosin filament sliding within the cardiac sarcomere, creating the force necessary for heart contraction. The underlying molecular mechanisms causing HCM phenotype remain elusive, therefore, there is an urgent need f… more
Date: May 2022
Creator: Strimaityte, Dovile

Nanolithographic Approaches to Probing Cell Membrane Modulation

Description: Metastatic cancer is more dangerous and difficult to treat than pre-metastatic cancer. Ninety percent of cancer-related deaths are caused by metastatic cancer. When cells go through metastases, they go through changes that allow them to break away from the primary tumor and invade secondary tissues. These changes, in lipid membrane composition and cellular glycocalyx, make the cell more resistant to therapeutics. Actin cytoskeleton contractility plays a major role in these changes, as increased… more
This item is restricted from view until June 1, 2024.
Date: May 2022
Creator: Mathis, Katelyn

Stem Cell Regulation Using Nanofibrous Membranes with Defined Structure and Pore Size

Description: Electrospun nanofibers have been researched extensively in the culturing of stem cells to understand their behavior since electrospun fibers mimic the native extracellular matrix (ECM) in many types of mammalian tissues. Here, electrospun nanofibers with defined structure (orientation/alignment) and pore size could significantly modulate human mesenchymal stem cell (hMSC) behavior. Controlling the fiber membrane pore size was predominantly influenced by the duration of electrospinning, while th… more
This item is restricted from view until September 1, 2024.
Date: August 2022
Creator: Blake, Laurence A
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