Purification of HMG-CoA Reductase and Regulation by Protein-Lipid Interactions
Description:
The enzyme 3-Hydroxy-3- Methylglutaryl Coenzyme A Reductase catalyzes the rate limiting step of hepatic cholesterol biosynthesis and is unique among the enzymes in the early part of the pathway in that it is membrane bound. This gives rise to potential regulation of the enzyme through interactions with the endoplasmic reticulum membrane. A purification procedure has been developed which consistently produces enzyme of high specific activity. In order to fully characterize the interactions betwe…
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Date:
December 1982
Creator:
Brent, Lynn G. (Lynn Gran)