Effect of Increasing Nitrogen Deposition on Soil Microbial Communities Metadata

Title

• Main Title Effect of Increasing Nitrogen Deposition on Soil Microbial Communities

Creator

• Author: Xiao, Shengmu
  Creator Type: Personal
• Author: Xue, Kai
  Creator Type: Personal
• Author: He, Zhili
  Creator Type: Personal
• Author: VanNostrand, Joy D.
  Creator Type: Personal
• Author: Liu, Jianshe
  Creator Type: Personal
• Author: Hobbie, Sarah E.
  Creator Type: Personal
• Author: Reich, Peter B.
  Creator Type: Personal
• Author: Zhou, Jizhong
  Creator Type: Personal

Contributor

• Sponsor: Lawrence Berkeley National Laboratory. Physical Biosciences Division.
  Contributor Type: Organization

Publisher

• Name: Lawrence Berkeley National Laboratory
  Place of Publication: Berkeley, California
  Additional Info: Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (United States)

Date

• Creation: 2010-05-17

Language

• English

Description

• Content Description: Increasing nitrogen deposition, increasing atmospheric CO2, and decreasing biodiversity are three main environmental changes occurring on a global scale. The BioCON (Biodiversity, CO2, and Nitrogen) ecological experiment site at the University of Minnesota's Cedar Creek Ecosystem Science Reserve started in 1997, to better understand how these changes would affect soil systems. To understand how increasing nitrogen deposition affects the microbial community diversity, heterogeneity, and functional structure impact soil microbial communities, 12 samples were collected from the BioCON plots in which nitrogenous fertilizer was added to simulate the effect of increasing nitrogen deposition and 12 samples from without added fertilizer. DNA from the 24 samples was extracted using a freeze-grind protocol, amplified, labeled with a fluorescent dye, and then hybridized to GeoChip, a functional gene array containing probes for genes involved in N, S and C cycling, metal resistance and organic contaminant degradation. Detrended correspondence analysis (DCA) of all genes detected was performed to analyze microbial community patterns. The first two axes accounted for 23.5percent of the total variation. The samples fell into two major groups: fertilized and non-fertilized, suggesting that nitrogenous fertilizer had a significant impact on soil microbial community structure and diversity. The functional gene numbers detected in fertilized samples was less that detected in non-fertilizer samples. Functional genes involving in the N cycling were mainly discussed.
• Physical Description: 1

Subject

• Keyword: Species Diversity
• Keyword: Dna
• STI Subject Categories: 59
• STI Subject Categories: 54
• Keyword: Fertilizers
• Keyword: Nitrogen Deposition, Increasing Atmospheric Co2, And Decreasing Biodiversity, Biocon
• Keyword: Streams Nitrogen Deposition, Increasing Atmospheric Co2, And Decreasing Biodiversity, Biocon
• Keyword: Carbon Cycle
• Keyword: Carbon Dioxide
• Keyword: Soils
• Keyword: Deposition
• Keyword: Earth Atmosphere
• Keyword: Nitrogen
• Keyword: Ecosystems
• Keyword: Microorganisms
• Keyword: Global Aspects
• Keyword: Genes

Source

• Conference: 110th General Meeting of the American Society for Microbiology, San Diego, CA, May 2010

Collection

• Name: Office of Scientific & Technical Information Technical Reports
  Code: OSTI

Institution

• Name: UNT Libraries Government Documents Department
  Code: UNTGD

Resource Type

• Poster

Format

• Image

Identifier

• Report No.: LBNL-4672E-Poster
• Grant Number: DE-AC02-05CH11231
• Office of Scientific & Technical Information Report Number: 1023389
• Archival Resource Key: ark:/67531/metadc829364