Final Report: DOE award: ER64516-1031199-0013966 2007-2011 Genomic Structure, Metagenomics, Horizontal Gene Transfer, and Natural Diversity of Prochlorococcus and Vibrio Page: 1 of 11
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Final Report:
DOE award: ER64516-1031199-0013966
2007-2011
Genomic Structure, Metagenomics, Horizontal Gene Transfer, and
Natural Diversity of Prochlorococcus and Vibrio
PIs
Sallie Chisholm, Eric Alm and Martin Polz
MIT
Our overarching goal with this proposal was to develop a deep understanding of the design of
Prochlorococcus and Vibrio cells, the variations in their designs, and the constraints that have
shaped this variation at the cell-environment interface. That is, we wanted to develop our
understanding of the biology of these microbes at all scales of biological organization, from
individual cell design to the dynamics of large populations.
The complete list of publications that were supported in whole or in part from this grant are
listed at the end of this document, and copies of the papers are supplied separately. Below we
summarize some of the more salient findings.
Aim I: Identify the patterns of genome diversity within and among natural Prochlorococcus
and Vibrio populations, and relate these patterns to the cellular metabolism, population
genetics, and the ecology of these groups;
Prochlorococcus
We have assembled the genomes of 12 Prochlorococcus single-cells from the south Pacific
ocean, which are part of two new high-light adapted phylogenic groups (Malmstrom, Rodrigue et
al 2012). We have also flow-sorted and amplified the genomes of hundred of individual cells
from different depths at the same location and at 3 different times during the same year (Kashtan
et al in prep). Another important study was to compare the Prochlorococcus metagenomes from
the Pacific and Atlantic oceans. Our results revealed that a major difference resides in phosphate
acquisition genes, demonstrating that DNA sequence data can help inform selective pressure in
the environment (Coleman and Chisholm 2010). We have also isolated new Prochlorococcus
strains based on specific nutritional requirements. For example, we now have obtained nitrate-
assimilating strains, which were long thought not to exist in Prochlorococcus and could play
important ecological roles. We have recently sequenced the genomes of these new strains and
these results will be published soon (Berube et al, in prep).
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Chisholm, Sally; Polz, Martin F. & Alm, Eric J. Final Report: DOE award: ER64516-1031199-0013966 2007-2011 Genomic Structure, Metagenomics, Horizontal Gene Transfer, and Natural Diversity of Prochlorococcus and Vibrio, text, August 9, 2013; United States. (https://digital.library.unt.edu/ark:/67531/metadc828284/m1/1/: accessed July 18, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.