Identification of Genes Involved in Flocculation by Whole Genome Sequencing of Thauera aminoaromatica Strain MZ1T Floc-defective Mutants Page: VIII
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LIST OF FIGURES
Page
Figure 2.1 Activated sludge wastewater treatment system..............................................................6
Figure 2.2 V iscous bulking sludge..........................................................................................................8
Figure 2.3 Basic components of T. aminoaromatica MZ1T exopolysaccha ride............................10
Figure 2.4 Mechanism of Wzx/Wzy-dependent group 1 capsular polysaccharide secretion.....15
Figure 2.5 Mechanisms of lipid carrier independent polysaccharide biosynthesis......................16
Figure 2.6 Cost per megabase of sequencing, from 2001 to 2015..................................................26
Figure 2.7 Principles and elements of semiconductor sequencing................................................29
Figure 2.8 Hom opolym er accuracy......................................................................................................31
Figure 2.9 Experimental workflow for the semiconductor sequencing.........................................33
Figure 2.10 Autom atic em ulsion PCR technology.............................................................................34
Figure 3.1 Chemically deacetylation reaction occurs in high basic concentration at 100 C......49
Figure 4.1. Ion torrent chip loading density of MZ1T 39A and 20A mutants................................54
Figure 4.2. Read length histogram of MZ1T 39A and 20A mutant genomic libraries..................55
Figure 4.3 Distribution of average sequence quality scores of MZ1T mutants.............................57
Figure 4.4 Reading maps of MZ1T 39A and 20A mutants................................................................58
Figure 4.5 Sanger sequencing verification of the Ion torrent sequence........................................70
Figure 4.6 Blast results of mzlt_3249 EPS deacetylase gene..........................................................71viii
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Prombutara, Pinidphon. Identification of Genes Involved in Flocculation by Whole Genome Sequencing of Thauera aminoaromatica Strain MZ1T Floc-defective Mutants, dissertation, December 2015; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc822777/m1/9/: accessed July 17, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .