The Possibility of Branch Conformation in Azotobacter Vinelandii Chromosomal DNA Carrying Multiple Gene Copies and Its Folded State in the Cell Page: 3
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3
rules applied.
Genetic redundancy in the A. vinelandii cell can explain the
difficulty in obtaining mutants. That is, if there are many copies of
each gene, the organism will not behave as a haploid organism as
does E. coli but rather as a eukaryotic cell with allelic genes. In
this view, in order to obtain a mutation in A. vinelandii, all copies
of the same gene would have to mutate at the same place at the
same time. The probability of this happening is quite large. If A.
vinelandii had 500 genes and if it had 27 copies of each gene, the
probability of all 27 alleles mutating during one generation would be
1+ 50027. If the mutations accumulated from one generation to the
next, the probability would be the same. If 1 x 106 gene mutations
occur during a given time, it would take 1+ 50027 x 1 x 106 intervals
of time to achieve a mutation that would affect phenotype.
Mutants in A. vinelandii sometimes tend to revert to wild type
even after several subcultures (32, 40, 42, 65, 75). This kind of
instability of mutant phenotypes in addition to the difficulty in
isolating mutants must be considered as a separate mechanism since
mutation indicates the presence of only one gene, i.e., haploid
condition. No further comment will be made on mutant reversion.
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Choi, Munhyeong. The Possibility of Branch Conformation in Azotobacter Vinelandii Chromosomal DNA Carrying Multiple Gene Copies and Its Folded State in the Cell, thesis or dissertation, August 1991; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc798271/m1/9/: accessed July 18, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .