The Possibility of Branch Conformation in Azotobacter Vinelandii Chromosomal DNA Carrying Multiple Gene Copies and Its Folded State in the Cell Page: 5
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strains and two isolated from water, possessed double-stranded,
covalently bonded, circular (plasmid) DNA. The six cultures were not
affected in their ability to produce nitrogenase and nitrogenase
reductase nor to fix nitrogen when they were cured of their plasmids
(39). Also, the non-nitrogen-fixing mutant, UW-1, contained a
plasmid but it too showed no differences in its ability to fix
nitrogen between plasmid-bearing and cured cultures (39). Maia et
al. concluded that there was no basis for Medhora's statement (41)
regarding nitrogen fixation and plasmids.
Punita et al. (47) reported that leu B, nif H, nif D, and nif K
genes appeared in at least 80 copies of each gene in stationary-
phase cultures of A. vinelandii. They used Southern blotting of A.
vinelandii chromosomal DNA probed with 12P-labeled nif H, D, and K
fragments, respectively, isolated from the cloned plasmid pMP2
carrying the nif HDK gene fragments of A. vinelandii. In these
studies, the intensity of the autoradiograms of A. vinelandii
chromosomes Was similar to the control, standard pMP2 DNA,
prepared as the equivalent of the 80-copy material (47).
The 1-Lactamase gene found on ampicillin resistant pMP2 or
pMM113 plasmids (47) was inserted into the A. vinelandii
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Choi, Munhyeong. The Possibility of Branch Conformation in Azotobacter Vinelandii Chromosomal DNA Carrying Multiple Gene Copies and Its Folded State in the Cell, thesis or dissertation, August 1991; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc798271/m1/11/: accessed July 18, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .