Radiation Effects Research Foundation five year strategic research plan and program management, 1997-2001 Page: 84 of 152
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is expected to be finished within four years.
Molecular and cellular biology is a rapidly moving field which requires constant upgrading
of technique. For the department of Radiobiology to maintain pace with the rest of the scientific
world, new methods of analysis have to be developed as well as introduced.
Establishment of in-cell PCR will allow careful scrutiny of individual cells for studying
EBV integration frequency or Bcl2 and BCR-ABL translocation in blood samples, for example.
Differential display is a potentially powerful technique that may reveal differences in transcription
of various genes between tumor and normal cells. The tumor and normal parts of archival tissue
sections can be separated and compared for changes that may have occurred in cancer related
genes as a result of A-bomb exposure. A similar comparison technique is comparative genomic
hybridization (CGH) which can scan the entire genome for possible changes, such as relatively
large (10 bp) deletions, in cancer DNA versus normal DNA.
The introduction of new technologies is also important as new problems arise. After
arrival of the new confocal microscope system, we will be able to study various new facets of
radiation effects on cellular function and cellular structure. New studies that can be initiated are
alterations of signal transduction in radiation induced tumors or molecular analysis of a single or
a few cells from surgical and paraffin embedded archival tissues of the survivors. Such abilities
will help to increase sensitivity and resolution for detection of changes in tissue samples.
Finally, it should be noted that most of the studies in the Department of Radiobiology
cannot be performed without collaboration with the Departments of Clinical Studies, Genetics,
Epidemiology, and Statistics. In view of RERF's limited resources it is important for us to
develop a general research plan that defines specific projects that can be done at RERF and
projects on which collaboration is important and establishes mechanisms for seeking this
collaboration and, where necessary, support.
Project time lines
1997 1998 1999 2000 2001
a) Genetic background
b) Non-cancer diseases = =
c) Human carcinogenesis =
d) Susceptibility to radiation == =
e) Clonal expansion =0 =
f)Viralimmunity
g) Somatic mutations
h) Immunity to oncogene products
I) Somatic mutation in-utero = = = =
Personnel requirements
The present staff is barely adequate to conduct the studies in radiobiology outlined above.
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Radiation Effects Research Foundation five year strategic research plan and program management, 1997-2001, report, October 31, 1997; Washington D.C.. (https://digital.library.unt.edu/ark:/67531/metadc716813/m1/84/: accessed July 16, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.