Radiation Effects Research Foundation five year strategic research plan and program management, 1997-2001 Page: 24 of 152
Medium: P; Size: vp.View a full description of this report.
Extracted Text
The following text was automatically extracted from the image on this page using optical character recognition software:
730 We plan to continue our studies of the features and mechanisms of radiation-induced
731 disorders in the hematolymphoid system at the cellular and molecular levels. These studies
732 include radiation effects on the distribution of T-cell subsets in the survivors and of radiation
733 effects on endocrine and hematopoietic growth factor levels as well as the study of stem cells, the
734 progenitors of cells which constitute the immune system.
735
736 Heritable Mutations
737
738 Permanent lymphocyte cell line cultures
739
740 The 1984 Genetic Study Conference endorsed an RERF plan to establish immortalized B-
741 lymphocyte cell lines from 1000 families (500 with at least one parent exposed within 2,000 m).
742 A sampling plan was drafted based on the T65D dosimetry. At this time the Biochemical Genetics
743 Laboratory has established cell lines for 800 families (1600 parents and 1200 children) based on
744 this plan. With the introduction of DS86 doses it was discovered that dose estimates were
745 unavailable for one or more of the parents in the remaining families. Over the next two years 200
746 additional Hiroshima and Nagasaki families for whom the parental doses are known will be
747 identified and efforts commenced to establish cell lines for these families in order to achieve the
748 goal of 1000 families. This is the largest cohort-based sample for the detection of radiation effects
749 on the human germline anywhere in the world.
750
751 Pilot studies of methods for the detection of deletion-insertion-rearrangement mutations (D/I/R)
752
753 Methods for genome-level (DNA) analyses are developing rapidly. It is likely that the next
754 five years will see the development of new markers and increasingly powerful methods that can
755 be used in the search for evidence of mutation in the children of the survivors. At this time, we
756 are examining several promising approaches. In each approach, mutations are detected by
757 comparison of a child's gel with that of his or her parents. Indications of a mutation include the
758 absence or dislocation of bands or spots as well as changes in intensity. As we learn of (or
759 develop) additional methods it may be necessary to initiate new pilot studies, in conjunction with
760 researchers outside of RERF when necessary.
761
762 Mutations at minisatellite loci
763
764 An initial study of 100 families will be extended by the addition of 100 families in order
765 to provide information for further comparisons of the RERF data with the results of a Belarus-UK
766 study (Dubrova et al 1996, Satoh et al 1996) that has been interpreted as providing evidence of
767 an excess of such mutations among Chernobyl victims.
768
769 Chemiluminescent bands on Southern filters
770
771 Employing a quantitative analysis of chemiluminescent bands on Southern filters, material
772 from the 200 families used in the minisatellite studies will also be screened for evidence of
773 mutation using DNA probes that correspond to the human counterparts of the seven mouse-
774 specific loci and other loci including genes that are suspected to be related to several common
775 chronic diseases, such as hypertension, diabetes mellitus, and hereditary nonpolyposis colorectal
776 cancer.17
W__
Upcoming Pages
Here’s what’s next.
Search Inside
This report can be searched. Note: Results may vary based on the legibility of text within the document.
Tools / Downloads
Get a copy of this page or view the extracted text.
Citing and Sharing
Basic information for referencing this web page. We also provide extended guidance on usage rights, references, copying or embedding.
Reference the current page of this Report.
Radiation Effects Research Foundation five year strategic research plan and program management, 1997-2001, report, October 31, 1997; Washington D.C.. (https://digital.library.unt.edu/ark:/67531/metadc716813/m1/24/: accessed July 16, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.