Engineered Microbial Consortium for the Efficient Conversion of Biomass to Biofuels Page: 83
ix, 120 pages : illustrations (some color)View a full description of this dissertation.
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0.10
0-06 -
0.04 -
0.02 -O0 0
I
(A)
o 10
o.08
0.06
0.04
o 02
000I
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Batch 1 Batch 2 Batch 3
S Day's
- @rrridium - 14 pAA 1
EIZZIC o 0 xd u!-ZbM14WT
-TBatch 1
Batch 3
Batchys
10 Days(B)
Figure 5.17: Ethanol production after 5 (A) and 10 (B) days using microcrystalline cellulose as a
carbon source. Error bars represent standard deviations among three replicates.
In the co-culture, figure 5.17, ethanol production was detected for Clostridium with ZM4
pAA1 for the three batches after 5 days and increased after 10 days while Clostridium with ZM4
wild type produced ethanol in batch 1 and batch 2 and not batch 3 and only detected after 10 days.
This observation clearly suggests that the co-culture involving recombinant ZM4 is more efficient
than the wild type. This can be seen from both the time it took for ethanol to be detected and the
quantity of ethanol detected. There appear to be only slight increases in ethanol production from
the co-culture involving the recombinant ZM4 from the the 10-day culture compared to that83
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Anieto, Ugochukwu Obiakornobi. Engineered Microbial Consortium for the Efficient Conversion of Biomass to Biofuels, dissertation, August 2014; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc699973/m1/94/: accessed July 17, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .