Engineered Microbial Consortium for the Efficient Conversion of Biomass to Biofuels Page: 57
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4.2 Glucose Standard Curve
Figure 4.2 below shows a plot of the glucose standard curve without ethanol, with 10%
ethanol, and with 20% ethanol. The regression values under the three conditions were determined
using the R programme to be 0.992, 0.993 and 0.992 respectively. This experiment was repeated
two more times and designated as batches 1, 2 and 3 with each batch consisting of glucose
standard 0%, 10% and 20% ethanol. To further determine whether the addition of ethanol had any
significant effect on the measurement of Absorbance in the reducing sugar reaction for the standard
curves, ANOVA was applied to each batch (a = 0.05) and the values obtained were 0.9994, 0.9962
and 0.9916, respectively. From the values obtained, it was concluded that ethanol up to 20% did
not interfere with the reducing sugar quantification reaction and would therefore not affect the
determination of the endoglucanase activity of the crude cellulosomes.
0.6
- - 0
0.5 -10
-X- 20
0.4-
0.2
0.1
0.0 I I I I
0 0.25 0.5 0.75 1
ing nil 1
Figure 4.2: Glucose standard curve in 0%, 10%, and 20% ethanol concentrations.57
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Anieto, Ugochukwu Obiakornobi. Engineered Microbial Consortium for the Efficient Conversion of Biomass to Biofuels, dissertation, August 2014; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc699973/m1/68/: accessed July 17, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .